Electrochemical impedance spectroscopy based biosensor for
rapid detection of Salmonella

D 2016

Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA and Petr SKLÁDAL

Basic information

Original name

Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella

Authors

FARKA, Zdeněk (203 Czech Republic, guarantor, belonging to the institution), Tomáš JUŘÍK (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, belonging to the institution)

Edition

Brno, XVI. Workshop of Physical Chemists and Electrochemists, p. 66-69, 4 pp. 2016

Publisher

Masaryk University

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

storage medium (CD, DVD, flash disk)

RIV identification code

RIV/00216224:14740/16:00090128

Organization unit

Central European Institute of Technology

ISBN

978-80-210-8267-0

Keywords in English

immunosensor; electrochemical impedance spectroscopy; food safety; Salmonella Typhimurium

Abstract

V originále

A label-free biosensor based on electrochemical impedance spectroscopy (EIS) was developed for the detection of Salmonella Typhimurium in milk. Specific antibody was immobilized on a screen-printed electrode, the electrode was incubated directly with the sample and binding of bacteria was measured as a change of impedance. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy (AFM) was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. A wide linear response was obtained in the range between 10^3 and 10^8 CFU·mL-1.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA and Petr SKLÁDAL. Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella. In Libuše Trnková. XVI. Workshop of Physical Chemists and Electrochemists. Brno: Masaryk University, 2016, p. 66-69. ISBN 978-80-210-8267-0.

@inproceedings{1347080,
   author = {Farka, Zdeněk and Juřík, Tomáš and Pastucha, Matěj and Skládal, Petr},
   address = {Brno},
   booktitle = {XVI. Workshop of Physical Chemists and Electrochemists},
   editor = {Libuše Trnková},
   keywords = {immunosensor; electrochemical impedance spectroscopy; food safety; Salmonella Typhimurium},
   howpublished = {paměťový nosič},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-8267-0},
   pages = {66-69},
   publisher = {Masaryk University},
   title = {Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella},
   year = {2016}
}

TY  - JOUR
ID  - 1347080
AU  - Farka, Zdeněk - Juřík, Tomáš - Pastucha, Matěj - Skládal, Petr
PY  - 2016
TI  - Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella
PB  - Masaryk University
CY  - Brno
SN  - 9788021082670
KW  - immunosensor
KW  - electrochemical impedance spectroscopy
KW  - food safety
KW  - Salmonella Typhimurium
N2  - A label-free biosensor based on electrochemical impedance spectroscopy (EIS) was developed for the detection of Salmonella Typhimurium in milk. Specific antibody was immobilized on a screen-printed electrode, the electrode was incubated directly with the sample and binding of bacteria was measured as a change of impedance. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy (AFM) was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. A wide linear response was obtained in the range between 10^3 and 10^8 CFU·mL-1.
ER  -

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA and Petr SKLÁDAL. Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella. In Libuše Trnková. \textit{XVI. Workshop of Physical Chemists and Electrochemists}. Brno: Masaryk University, 2016, p.~66-69. ISBN~978-80-210-8267-0.

Detailed Information on Publication Record