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@inproceedings{1347080,
author = {Farka, Zdeněk and Juřík, Tomáš and Pastucha, Matěj and Skládal, Petr},
address = {Brno},
booktitle = {XVI. Workshop of Physical Chemists and Electrochemists},
editor = {Libuše Trnková},
keywords = {immunosensor; electrochemical impedance spectroscopy; food safety; Salmonella Typhimurium},
howpublished = {paměťový nosič},
language = {eng},
location = {Brno},
isbn = {978-80-210-8267-0},
pages = {66-69},
publisher = {Masaryk University},
title = {Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella},
year = {2016}
}
TY - JOUR
ID - 1347080
AU - Farka, Zdeněk - Juřík, Tomáš - Pastucha, Matěj - Skládal, Petr
PY - 2016
TI - Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella
PB - Masaryk University
CY - Brno
SN - 9788021082670
KW - immunosensor
KW - electrochemical impedance spectroscopy
KW - food safety
KW - Salmonella Typhimurium
N2 - A label-free biosensor based on electrochemical impedance spectroscopy (EIS) was developed for the detection of Salmonella Typhimurium in milk. Specific antibody was immobilized on a screen-printed electrode, the electrode was incubated directly with the sample and binding of bacteria was measured as a change of impedance. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy (AFM) was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. A wide linear response was obtained in the range between 10^3 and 10^8 CFU·mL-1.
ER -
FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA and Petr SKLÁDAL. Electrochemical impedance spectroscopy based biosensor for rapid detection of Salmonella. In Libuše Trnková. \textit{XVI. Workshop of Physical Chemists and Electrochemists}. Brno: Masaryk University, 2016, p.~66-69. ISBN~978-80-210-8267-0.
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