Competitive Upconversion-Linked Immunosorbent Assay for the
Sensitive Detection of Diclofenac

J 2016

Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac

HLAVÁČEK, Antonín, Zdeněk FARKA, Maria HUEBNER, Veronika HORŇÁKOVÁ, Daniel NĚMEČEK et. al.

Basic information

Original name

Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac

Authors

HLAVÁČEK, Antonín (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution), Maria HUEBNER (276 Germany), Veronika HORŇÁKOVÁ (203 Czech Republic, belonging to the institution), Daniel NĚMEČEK (203 Czech Republic, belonging to the institution), Reinhard NIESSNER (276 Germany), Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution), Dietmar KNOPP (276 Germany) and Hans Heiner GORRIS (276 Germany)

Edition

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2016, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.320

RIV identification code

RIV/00216224:14740/16:00090133

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/acs.analchem.6b01083

UT WoS

000377631000065

Keywords in English

antibody; bioconjugation; diclofenac; immunoassay; pharmaceutical micropollutant; photon-upconversion

Abstract

V originále

Photon-upconverting nanoparticles (UCNPs) emit light of shorter wavelength under near-infrared excitation and thus avoid optical background interference. We have exploited this unique photophysical feature to establish a sensitive competitive immunoassay for the detection of the pharmaceutical micropollutant diclofenac (DCF) in water. The so-called upconversion-linked immunosorbent assay (ULISA) was critically dependent on the design of the upconversion luminescent detection label. Silica-coated UCNPs (50 nm in diameter) exposing carboxyl groups on the surface were conjugated to a secondary anti-IgG antibody. We investigated the structure and monodispersity of the nanoconjugates in detail. Using a highly affine anti-DCF primary antibody, the optimized ULISA reached a detection limit of 0.05 ng DCF per mL. This performance came close to a conventional enzyme-linked immunosorbent assay (ELISA) without the need for an enzyme-mediated signal amplification step. The ULISA was further employed for analyzing drinking and surface water samples. The results were consistent with a conventional ELISA as well as liquid chromatography–mass spectrometry (LC–MS).

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

LD15023, research and development project

Name: Příprava diskrétních biokonjugátů photon-upconversion nanočástic

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

HLAVÁČEK, Antonín, Zdeněk FARKA, Maria HUEBNER, Veronika HORŇÁKOVÁ, Daniel NĚMEČEK, Reinhard NIESSNER, Petr SKLÁDAL, Dietmar KNOPP and Hans Heiner GORRIS. Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2016, vol. 88, No 11, p. 6011-6017. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.6b01083.

@article{1347122,
   author = {Hlaváček, Antonín and Farka, Zdeněk and Huebner, Maria and Horňáková, Veronika and Němeček, Daniel and Niessner, Reinhard and Skládal, Petr and Knopp, Dietmar and Gorris, Hans Heiner},
   article_location = {Washington, D.C., USA},
   article_number = {11},
   doi = {http://dx.doi.org/10.1021/acs.analchem.6b01083},
   keywords = {antibody; bioconjugation; diclofenac; immunoassay; pharmaceutical micropollutant; photon-upconversion},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac},
   url = {http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1347122
AU  - Hlaváček, Antonín - Farka, Zdeněk - Huebner, Maria - Horňáková, Veronika - Němeček, Daniel - Niessner, Reinhard - Skládal, Petr - Knopp, Dietmar - Gorris, Hans Heiner
PY  - 2016
TI  - Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac
JF  - Analytical Chemistry
VL  - 88
IS  - 11
SP  - 6011-6017
EP  - 6011-6017
PB  - American Chemical Society
SN  - 00032700
KW  - antibody
KW  - bioconjugation
KW  - diclofenac
KW  - immunoassay
KW  - pharmaceutical micropollutant
KW  - photon-upconversion
UR  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083
L2  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083
N2  - Photon-upconverting nanoparticles (UCNPs) emit light of shorter wavelength under near-infrared excitation and thus avoid optical background interference. We have exploited this unique photophysical feature to establish a sensitive competitive immunoassay for the detection of the pharmaceutical micropollutant diclofenac (DCF) in water. The so-called upconversion-linked immunosorbent assay (ULISA) was critically dependent on the design of the upconversion luminescent detection label. Silica-coated UCNPs (50 nm in diameter) exposing carboxyl groups on the surface were conjugated to a secondary anti-IgG antibody. We investigated the structure and monodispersity of the nanoconjugates in detail. Using a highly affine anti-DCF primary antibody, the optimized ULISA reached a detection limit of 0.05 ng DCF per mL. This performance came close to a conventional enzyme-linked immunosorbent assay (ELISA) without the need for an enzyme-mediated signal amplification step. The ULISA was further employed for analyzing drinking and surface water samples. The results were consistent with a conventional ELISA as well as liquid chromatography–mass spectrometry (LC–MS).
ER  -

HLAVÁČEK, Antonín, Zdeněk FARKA, Maria HUEBNER, Veronika HORŇÁKOVÁ, Daniel NĚMEČEK, Reinhard NIESSNER, Petr SKLÁDAL, Dietmar KNOPP and Hans Heiner GORRIS. Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2016, vol.~88, No~11, p.~6011-6017. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.6b01083.

Detailed Information on Publication Record