| HLAVÁČEK, Antonín, Zdeněk FARKA, Maria HUEBNER, Veronika HORŇÁKOVÁ, Daniel NĚMEČEK, Reinhard NIESSNER, Petr SKLÁDAL, Dietmar KNOPP and Hans Heiner GORRIS. Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2016, vol. 88, No 11, p. 6011-6017. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.6b01083. |
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@article{1347122,
author = {Hlaváček, Antonín and Farka, Zdeněk and Huebner, Maria and Horňáková, Veronika and Němeček, Daniel and Niessner, Reinhard and Skládal, Petr and Knopp, Dietmar and Gorris, Hans Heiner},
article_location = {Washington, D.C., USA},
article_number = {11},
doi = {http://dx.doi.org/10.1021/acs.analchem.6b01083},
keywords = {antibody; bioconjugation; diclofenac; immunoassay; pharmaceutical micropollutant; photon-upconversion},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac},
url = {http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083},
volume = {88},
year = {2016}
}
TY - JOUR
ID - 1347122
AU - Hlaváček, Antonín - Farka, Zdeněk - Huebner, Maria - Horňáková, Veronika - Němeček, Daniel - Niessner, Reinhard - Skládal, Petr - Knopp, Dietmar - Gorris, Hans Heiner
PY - 2016
TI - Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac
JF - Analytical Chemistry
VL - 88
IS - 11
SP - 6011-6017
EP - 6011-6017
PB - American Chemical Society
SN - 00032700
KW - antibody
KW - bioconjugation
KW - diclofenac
KW - immunoassay
KW - pharmaceutical micropollutant
KW - photon-upconversion
UR - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083
L2 - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01083
N2 - Photon-upconverting nanoparticles (UCNPs) emit light of shorter wavelength under near-infrared excitation and thus avoid optical background interference. We have exploited this unique photophysical feature to establish a sensitive competitive immunoassay for the detection of the pharmaceutical micropollutant diclofenac (DCF) in water. The so-called upconversion-linked immunosorbent assay (ULISA) was critically dependent on the design of the upconversion luminescent detection label. Silica-coated UCNPs (50 nm in diameter) exposing carboxyl groups on the surface were conjugated to a secondary anti-IgG antibody. We investigated the structure and monodispersity of the nanoconjugates in detail. Using a highly affine anti-DCF primary antibody, the optimized ULISA reached a detection limit of 0.05 ng DCF per mL. This performance came close to a conventional enzyme-linked immunosorbent assay (ELISA) without the need for an enzyme-mediated signal amplification step. The ULISA was further employed for analyzing drinking and surface water samples. The results were consistent with a conventional ELISA as well as liquid chromatography–mass spectrometry (LC–MS).
ER -
HLAVÁČEK, Antonín, Zdeněk FARKA, Maria HUEBNER, Veronika HORŇÁKOVÁ, Daniel NĚMEČEK, Reinhard NIESSNER, Petr SKLÁDAL, Dietmar KNOPP and Hans Heiner GORRIS. Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2016, vol.~88, No~11, p.~6011-6017. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.6b01083.
|
