The occurrence of Toxoplasma gondii in domestic pigs and wild
boars slaughtered in the Czech Republic

a 2016

The occurrence of Toxoplasma gondii in domestic pigs and wild boars slaughtered in the Czech Republic

SLANÝ, Michal, Lea JAKUBCOVÁ, Nikol RESLOVÁ and Alena LORENCOVÁ

Basic information

Original name

The occurrence of Toxoplasma gondii in domestic pigs and wild boars slaughtered in the Czech Republic

Authors

SLANÝ, Michal, Lea JAKUBCOVÁ, Nikol RESLOVÁ and Alena LORENCOVÁ

Edition

12th European Multicolloquium of Parasitology (EMOP), 2016

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Finland

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords (in Czech)

Toxoplasma gondii; divoké prase; porážka

Keywords in English

Toxoplasma gondii; wild boars; slaughter

Abstract

V originále

Toxoplasma gondii is the fourth most important agent according to the global ranking of foodborne parasites. The aim of this study was to survey the presence of T. gondii in pigs and wild boars bred in different production systems in the Czech Republic. ELISA, qPCR (529 rep and B1) and nested PCRs followed by RFLP (Apico, BTUB, GRA6, L358, PK1, SAG2 and SAG3) were applied. Diaphragm samples from total of 499 animals were collected during years 2012-2015. Pigs came from one organic (n=48) and four conventional farms (n=61). Wild boars originated from three game preserves (n=356) and two free hunting areas (n=34) in the Czech Republic. The higher incidence of T. gondii was observed in pigs from organic farm (29.2%) when compared to conventional farms (14.8%). It is in accordance with published data suggesting that intensive production systems have eliminated Toxoplasma infection in pigs. However, organic farming where pigs are not separated from the outside environment may lead to reemergence of this pathogen. The presence of T. gondii was also confirmed in wild boars, but with no significant difference between game preserves (9.8%) and free hunting areas (8.8%). Further genotyping revealed present genotype II only. To conclude, T. gondii infection is widespread in pigs and wild boars bred and slaughtered in the Czech Republic. The results show that pork and venison poses a certain health risk and can be a potential source of T. gondii for humans.

Links

LO1218, research and development project

Name: Zdravé zvíře jako zdroj zdravé potraviny

MUNI/A/1325/2015, interní kód MU

Name: Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí (Acronym: BIDA5)

Investor: Masaryk University, Category A

QJ1210113, research and development project

Name: Vliv tradičních a netradičních způsobů zpracování masa hospodářských a volně žijících zvířat na výskyt nově hrozících alimentárních virových, bakteriálních a parazitárních agens ve finálních produktech

Citovat

SLANÝ, Michal, Lea JAKUBCOVÁ, Nikol RESLOVÁ and Alena LORENCOVÁ. The occurrence of Toxoplasma gondii in domestic pigs and wild boars slaughtered in the Czech Republic. In 12th European Multicolloquium of Parasitology (EMOP). 2016.

@proceedings{1347257,
   author = {Slaný, Michal and Jakubcová, Lea and Reslová, Nikol and Lorencová, Alena},
   booktitle = {12th European Multicolloquium of Parasitology (EMOP)},
   keywords = {Toxoplasma gondii; wild boars; slaughter},
   language = {eng},
   title = {The occurrence of Toxoplasma gondii in domestic pigs and wild boars slaughtered in the Czech Republic},
   year = {2016}
}

TY  - CONF
ID  - 1347257
AU  - Slaný, Michal - Jakubcová, Lea - Reslová, Nikol - Lorencová, Alena
PY  - 2016
TI  - The occurrence of Toxoplasma gondii in domestic pigs and wild boars slaughtered in the Czech Republic
KW  - Toxoplasma gondii
KW  - wild boars
KW  - slaughter
N2  - Toxoplasma gondii is the fourth most important agent according to the global ranking of foodborne parasites. The aim of this study was to survey the presence of T. gondii in pigs and wild boars bred in different production systems in the Czech Republic. ELISA, qPCR (529 rep and B1) and nested PCRs followed by RFLP (Apico, BTUB, GRA6, L358, PK1, SAG2 and SAG3) were applied. Diaphragm samples from total of 499 animals were collected during years 2012-2015. Pigs came from one organic (n=48) and four conventional farms (n=61). Wild boars originated from three game preserves (n=356) and two free hunting areas (n=34) in the Czech Republic. The higher incidence of T. gondii was observed in pigs from organic farm (29.2%) when compared to conventional farms (14.8%). It is in accordance with published data suggesting that intensive production systems have eliminated Toxoplasma infection in pigs. However, organic farming where pigs are not separated from the outside environment may lead to reemergence of this pathogen. The presence of T. gondii was also confirmed in wild boars, but with no significant difference between game preserves (9.8%) and free hunting areas (8.8%). Further genotyping revealed present genotype II only. To conclude, T. gondii infection is widespread in pigs and wild boars bred and slaughtered in the Czech Republic. The results show that pork and venison poses a certain health risk and can be a potential source of T. gondii for humans.
ER  -

SLANÝ, Michal, Lea JAKUBCOVÁ, Nikol RESLOVÁ and Alena LORENCOVÁ. The occurrence of Toxoplasma gondii in domestic pigs and wild boars slaughtered in the Czech Republic. In \textit{12th European Multicolloquium of Parasitology (EMOP)}. 2016.

Detailed Information on Publication Record