RESLOVÁ, Nikol, Martin KAŠNÝ, Michal SLANÝ, Silvia MONTEIRO and Ricardo SANTOS. Real-time PCR a digital PCR v diagnostice parazitů v potravinách (Real-time PCR a digital PCR in disgnostics of parasitological agents in food). In XV. Tomáškovy dny mladých mikrobiologů. 2016. ISBN 978-80-210-8255-7.
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Basic information
Original name Real-time PCR a digital PCR v diagnostice parazitů v potravinách
Name in Czech Real-time PCR a digital PCR v diagnostice parazitů v potravinách
Name (in English) Real-time PCR a digital PCR in disgnostics of parasitological agents in food
Authors RESLOVÁ, Nikol (203 Czech Republic, guarantor, belonging to the institution), Martin KAŠNÝ (203 Czech Republic, belonging to the institution), Michal SLANÝ (203 Czech Republic), Silvia MONTEIRO (620 Portugal) and Ricardo SANTOS (620 Portugal).
Edition XV. Tomáškovy dny mladých mikrobiologů, 2016.
Other information
Original language Czech
Type of outcome Presentations at conferences
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/16:00090148
Organization unit Faculty of Science
ISBN 978-80-210-8255-7
Keywords (in Czech) real-time PCR; digitální PCR; diagnostika potravinových parazitů; giardia intestinalis; toxoplasma gondii
Keywords in English real-time PCR; digital PCR; diagnostics of foodborne parasites; giardia intestinalis; toxoplasma gondii
Changed by Changed by: RNDr. Martin Kašný, Ph.D., učo 11259. Changed: 25/3/2017 08:10.
Abstract
In recent years, the increase of reported outbreaks causing human-diseases associated with food-borne parasitic infections (uni/multi-cellular) arising from meat consumption has been recorded. This trend could be affected e.g. by the changes in farming management towards bio-production, globalization of food market, global climate change and also by the meat processing. This situation is consequently inducing the need for improvement of the diagnostic applications. Our work is focused on development of a reliable comprehensive molecular diagnostic method useful for rapid control of meat products. We adopted high sensitive multiplex oligonucleotide ligation-PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures of parasites from complex samples and xMAP technology representing a novel detection platform based on magnetic microspheres. Visualization of the products is then realized via qualitative and quantitative MAGPIX instrumentation. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA originating from two parasitic worms - Trichinella spiralis (nematode; partial sequence of 18S rRNA gene) and Taenia saginata (cestode; partial sequence of mitochondrial COX1 gene) were designed. Reaction conditions of MOL-PCR were optimized for simultaneous duplex assay. Currently, the calibration of MAGPIX system is in process.
Abstract (in English)
In recent years, the increase of reported outbreaks causing human-diseases associated with food-borne parasitic infections (uni/multi-cellular) arising from meat consumption has been recorded. This trend could be affected e.g. by the changes in farming management towards bio-production, globalization of food market, global climate change and also by the meat processing. This situation is consequently inducing the need for improvement of the diagnostic applications. Our work is focused on development of a reliable comprehensive molecular diagnostic method useful for rapid control of meat products. We adopted high sensitive multiplex oligonucleotide ligation-PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures of parasites from complex samples and xMAP technology representing a novel detection platform based on magnetic microspheres. Visualization of the products is then realized via qualitative and quantitative MAGPIX instrumentation. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA originating from two parasitic worms - Trichinella spiralis (nematode; partial sequence of 18S rRNA gene) and Taenia saginata (cestode; partial sequence of mitochondrial COX1 gene) were designed. Reaction conditions of MOL-PCR were optimized for simultaneous duplex assay. Currently, the calibration of MAGPIX system is in process.
Links
MUNI/A/1325/2015, interní kód MUName: Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí (Acronym: BIDA5)
Investor: Masaryk University, Category A
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