Detailed Information on Publication Record
2016
Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows
KAŠPAROVSKÁ, Jitka, Martina ZAPLETALOVÁ, Kateřina DADÁKOVÁ, Ludmila KŘÍŽOVÁ, Sylvie HADROVÁ et. al.Basic information
Original name
Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows
Authors
KAŠPAROVSKÁ, Jitka (203 Czech Republic, belonging to the institution), Martina ZAPLETALOVÁ (203 Czech Republic, belonging to the institution), Kateřina DADÁKOVÁ (203 Czech Republic, belonging to the institution), Ludmila KŘÍŽOVÁ (203 Czech Republic, belonging to the institution), Sylvie HADROVÁ (203 Czech Republic, belonging to the institution), Matej LEXA (703 Slovakia, belonging to the institution), Jan LOCHMAN (203 Czech Republic, belonging to the institution) and Tomáš KAŠPAROVSKÝ (203 Czech Republic, belonging to the institution)
Edition
Plos one, San Francisco, Public Library of Science, 2016, 1932-6203
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.806
RIV identification code
RIV/00216224:14310/16:00087977
Organization unit
Faculty of Science
UT WoS
000375211700120
Keywords in English
dairy cow; soybean extract; equol; carry-over rate; rumen; bacterial phylogenetics
Změněno: 5/4/2017 10:51, Ing. Andrea Mikešková
Abstract
V originále
In this study, we compared the effects of two diets containing different isoflavone concentrations on the isoflavone transfer from feed into milk and on the rumen microbiota in lactating dairy cows. The on-farm experiment was conducted on twelve lactating Czech Fleckvieh x Holstein cows divided into two groups, each with similar mean milk yield. Twice daily, cows were individually fed a diet based on maize silage, meadow hay and supplemental mixture. Control group (CTRL) received the basal diet while the experimental group (EXP) received the basal diet supplemented with 40% soybean isoflavone extract. The average daily isoflavone intake in the EXP group (16 g/day) was twice as high as that in the CTRL group (8.4 g/day, P<0.001). Total isoflavone concentrations in milk from the CTRL and EXP groups were 96.89 and 276.07 mu g/L, respectively (P<0.001). Equol concentrations in milk increased from 77.78 mu g/L in the CTRL group to 186.30 mu g/L in the EXP group (P<0.001). The V3-4 region of bacterial 16S rRNA genes was used for metagenomic analysis of the rumen microbiome. The experimental cows exhibited fewer OTUs at a distance level of 0.03 compared to control cows (P<0.05) and reduced microbial richness compared to control cows based on the calculated Inverse Simpson and Shannon indices. Non-metric multidimensional scaling analysis showed that the major contributor to separation between the experimental and control groups were changes in the representation of bacteria belonging to the phyla Bacteroidetes, Proteobacteria, Firmicutes, and Planctomycetes. Surprisingly, a statistically significant positive correlation was found only between isoflavones and the phyla Burkholderiales (r = 0.65, P<0.05) and unclassified Betaproteobacteria (r = 0.58, P<0.05). Previous mouse and human studies of isoflavone effects on the composition of gastrointestinal microbial populations generally report similar findings.
Links
| GA13-34134S, research and development project |
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