Soybean-Derived Isoflavone Determination in Rumen Fluid and
Milk by LC–MS-(TOF).

J 2016

Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).

KAŠPAROVSKÁ, Jitka, Ludmila KŘÍŽOVÁ, Jan LOCHMAN, Kateřina DADÁKOVÁ, Tomáš KAŠPAROVSKÝ et. al.

Basic information

Original name

Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).

Authors

KAŠPAROVSKÁ, Jitka (203 Czech Republic, belonging to the institution), Ludmila KŘÍŽOVÁ (203 Czech Republic, belonging to the institution), Jan LOCHMAN (203 Czech Republic, belonging to the institution), Kateřina DADÁKOVÁ (203 Czech Republic, belonging to the institution) and Tomáš KAŠPAROVSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Chromatographic Science, Oxford University Press, 2016, 0021-9665

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.984

RIV identification code

RIV/00216224:14310/16:00087978

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1093/chromsci/bmw033

UT WoS

000385022100014

Keywords in English

Isoflavones; equol; rumen fluid; milk; HPLC-MS

Abstract

V originále

Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC–MS-(TOF). The samples of rumen fluid and milk were obtained from 12 lactating dairy cows ingesting 7,500–9,500 mg of total isoflavones daily. The validation of the developed method showed the limits of quantification to be in the range of 0.9–5.0 ng/mL. The precision was determined as relative standard deviation, which was lower than 25% in all cases. The recoveries of the most isoflavonoids were satisfactory. Lower recoveries of daidzin and glycitin can be solved by adding an internal standard. The presented method will be useful for kinetic studies of isoflavone metabolism in ruminants due to simultaneous quantification of free aglycones and glycosides in the rumen fluid.

Links

GA13-34134S, research and development project

Name: Metabolizmus izoflavonů v bachoru se zaměřením na obsah equolu v kravském mléce

Investor: Czech Science Foundation

Citovat

KAŠPAROVSKÁ, Jitka, Ludmila KŘÍŽOVÁ, Jan LOCHMAN, Kateřina DADÁKOVÁ and Tomáš KAŠPAROVSKÝ. Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF). Journal of Chromatographic Science. Oxford University Press, 2016, vol. 54, No 6, p. 997-1003. ISSN 0021-9665. Available from: https://dx.doi.org/10.1093/chromsci/bmw033.

@article{1347276,
   author = {Kašparovská, Jitka and Křížová, Ludmila and Lochman, Jan and Dadáková, Kateřina and Kašparovský, Tomáš},
   article_number = {6},
   doi = {http://dx.doi.org/10.1093/chromsci/bmw033},
   keywords = {Isoflavones; equol; rumen fluid; milk; HPLC-MS},
   language = {eng},
   issn = {0021-9665},
   journal = {Journal of Chromatographic Science},
   title = {Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).},
   volume = {54},
   year = {2016}
}

TY  - JOUR
ID  - 1347276
AU  - Kašparovská, Jitka - Křížová, Ludmila - Lochman, Jan - Dadáková, Kateřina - Kašparovský, Tomáš
PY  - 2016
TI  - Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).
JF  - Journal of Chromatographic Science
VL  - 54
IS  - 6
SP  - 997-1003
EP  - 997-1003
PB  - Oxford University Press
SN  - 00219665
KW  - Isoflavones
KW  - equol
KW  - rumen fluid
KW  - milk
KW  - HPLC-MS
N2  - Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC–MS-(TOF). The samples of rumen fluid and milk were obtained from 12 lactating dairy cows ingesting 7,500–9,500 mg of total isoflavones daily. The validation of the developed method showed the limits of quantification to be in the range of 0.9–5.0 ng/mL. The precision was determined as relative standard deviation, which was lower than 25% in all cases. The recoveries of the most isoflavonoids were satisfactory. Lower recoveries of daidzin and glycitin can be solved by adding an internal standard. The presented method will be useful for kinetic studies of isoflavone metabolism in ruminants due to simultaneous quantification of free aglycones and glycosides in the rumen fluid.
ER  -

KAŠPAROVSKÁ, Jitka, Ludmila KŘÍŽOVÁ, Jan LOCHMAN, Kateřina DADÁKOVÁ and Tomáš KAŠPAROVSKÝ. Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF). \textit{Journal of Chromatographic Science}. Oxford University Press, 2016, vol.~54, No~6, p.~997-1003. ISSN~0021-9665. Available from: https://dx.doi.org/10.1093/chromsci/bmw033.

Detailed Information on Publication Record