Are there multiple mechanisms of anaerobic sulfur oxidation
with ferric iron in Acidithiobacillus ferrooxidans?

J 2016

Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans?

KUČERA, Jiří, Eva PAKOSTOVÁ, Jan LOCHMAN, Oldřich JANICZEK, Martin MANDL et. al.

Basic information

Original name

Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans?

Name in Czech

Existuje více mechanismů anaerobní oxidace síry železitými ionty u bakterie Acidithiobacillus ferrooxidans?

Authors

KUČERA, Jiří (203 Czech Republic, belonging to the institution), Eva PAKOSTOVÁ (203 Czech Republic, belonging to the institution), Jan LOCHMAN (203 Czech Republic, belonging to the institution), Oldřich JANICZEK (203 Czech Republic, belonging to the institution) and Martin MANDL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Research in Microbiology, Amsterdam, Elsevier, 2016, 0923-2508

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.549

RIV identification code

RIV/00216224:14310/16:00087982

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.resmic.2016.02.004

UT WoS

000378671300003

Keywords (in Czech)

Acidithiobacillus ferrooxidans; anaerobní respirační dráha; sirný metabolismus; redukce Fe(III); RT qPCR

Keywords in English

Acidithiobacillus ferrooxidans; Anaerobic respiration pathway; Sulfur metabolism; Ferric iron reduction; RT qPCR

Abstract

ORIG CZ

V originále

To clarify the pathway of anaerobic sulfur oxidation coupled with dissimilatory ferric iron reduction in Acidithiobacillus ferrooxidans strain CCM 4253 cells, we monitored their energy metabolism gene transcript profiles. Several genes encoding electron transporters involved in aerobic iron and sulfur respiration were induced during anaerobic growth of ferrous iron-grown cells. Most sulfur metabolism genes were either expressed at the basal level or their expression declined. However, transcript levels of genes assumed to be responsible for processing of elemental sulfur and other sulfur intermediates were elevated at the beginning of the growth period. In contrast, genes with predicted functions in formation of hydrogen sulfide and sulfate were significantly repressed. The main proposed mechanism involves: outer membrane protein Cyc2 (assumed to function as a terminal ferric iron reductase); periplasmic electron shuttle rusticyanin; c4-type cytochrome CycA1; the inner membrane cytochrome bc1 complex I; and the quinone pool providing connection to the sulfur metabolism machinery, consisting of heterodisulfide reductase, thiosulfate:quinone oxidoreductase and tetrathionate hydrolase. However, an alternative mechanism seems to involve a high potential iron-sulfur protein Hip, c4-type cytochrome CycA2 and inner membrane cytochrome bc1 complex II. Our results conflict with findings regarding the type strain, indicating strain- or phenotype-dependent pathway variation.

In Czech

Studium anaerobní bakteriální oxidace síry železitými ionty. Na základě transkriptomických analýz se předpokládá přítomnost složek systému oxidujícího Fe(II), jež katalyzují i anaerobní proces.

Links

GP14-27075P, research and development project

Name: Mechanismus bakteriální anaerobní oxidace síry ovlivňující acidifikaci prostředí sulfidových odpadů

Investor: Czech Science Foundation

Citovat

KUČERA, Jiří, Eva PAKOSTOVÁ, Jan LOCHMAN, Oldřich JANICZEK and Martin MANDL. Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans? Research in Microbiology. Amsterdam: Elsevier, 2016, vol. 167, No 5, p. 357-366. ISSN 0923-2508. Available from: https://dx.doi.org/10.1016/j.resmic.2016.02.004.

@article{1347637,
   author = {Kučera, Jiří and Pakostová, Eva and Lochman, Jan and Janiczek, Oldřich and Mandl, Martin},
   article_location = {Amsterdam},
   article_number = {5},
   doi = {http://dx.doi.org/10.1016/j.resmic.2016.02.004},
   keywords = {Acidithiobacillus ferrooxidans; Anaerobic respiration pathway; Sulfur metabolism; Ferric iron reduction; RT qPCR},
   language = {eng},
   issn = {0923-2508},
   journal = {Research in Microbiology},
   title = {Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans?},
   volume = {167},
   year = {2016}
}

TY  - JOUR
ID  - 1347637
AU  - Kučera, Jiří - Pakostová, Eva - Lochman, Jan - Janiczek, Oldřich - Mandl, Martin
PY  - 2016
TI  - Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans?
JF  - Research in Microbiology
VL  - 167
IS  - 5
SP  - 357-366
EP  - 357-366
PB  - Elsevier
SN  - 09232508
KW  - Acidithiobacillus ferrooxidans
KW  - Anaerobic respiration pathway
KW  - Sulfur metabolism
KW  - Ferric iron reduction
KW  - RT qPCR
N2  - To clarify the pathway of anaerobic sulfur oxidation coupled with dissimilatory ferric iron reduction in Acidithiobacillus ferrooxidans strain CCM 4253 cells, we monitored their energy metabolism gene transcript profiles. Several genes encoding electron transporters involved in aerobic iron and sulfur respiration were induced during anaerobic growth of ferrous iron-grown cells. Most sulfur metabolism genes were either expressed at the basal level or their expression declined. However, transcript levels of genes assumed to be responsible for processing of elemental sulfur and other sulfur intermediates were elevated at the beginning of the growth period. In contrast, genes with predicted functions in formation of hydrogen sulfide and sulfate were significantly repressed. The main proposed mechanism involves: outer membrane protein Cyc2 (assumed to function as a terminal ferric iron reductase); periplasmic electron shuttle rusticyanin; c4-type cytochrome CycA1; the inner membrane cytochrome bc1 complex I; and the quinone pool providing connection to the sulfur metabolism machinery, consisting of heterodisulfide reductase, thiosulfate:quinone oxidoreductase and tetrathionate hydrolase. However, an alternative mechanism seems to involve a high potential iron-sulfur protein Hip, c4-type cytochrome CycA2 and inner membrane cytochrome bc1 complex II. Our results conflict with findings regarding the type strain, indicating strain- or phenotype-dependent pathway variation.
ER  -

KUČERA, Jiří, Eva PAKOSTOVÁ, Jan LOCHMAN, Oldřich JANICZEK and Martin MANDL. Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans? \textit{Research in Microbiology}. Amsterdam: Elsevier, 2016, vol.~167, No~5, p.~357-366. ISSN~0923-2508. Available from: https://dx.doi.org/10.1016/j.resmic.2016.02.004.

Detailed Information on Publication Record