Switching central trinucleotide sequences of DNA hepatmer
regulates adsorption on mercury electrode

J 2016

Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode

TŘÍSKOVÁ, Iveta, Libor GURECKÝ and Libuše TRNKOVÁ

Basic information

Original name

Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode

Authors

TŘÍSKOVÁ, Iveta (203 Czech Republic, belonging to the institution), Libor GURECKÝ (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Monatshefte für Chemie - Chemical Monthly, Wien, Springer-Verlag, 2016, 0026-9247

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.282

RIV identification code

RIV/00216224:14310/16:00090237

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00706-016-1689-7

UT WoS

000374172200003

Keywords (in Czech)

DNA heptamery; visící rtuťová kapková elektroda; voltametrie s lineární polarizací elektrody; eliminační voltametrická procedura; adsorpční izoterma; adsorpční koeficient

Keywords in English

DNA heptamers; hanging mercury drop electrode; linear sweep voltammetry; elimination voltammetric procedure; adsorption isotherm; adsorption coefficient

Abstract

V originále

The focus of the presented study is electrochemical investigation of DNA heptamers d(GCNNNGC) with a different center trinucleotide sequence (NNN = GAA, AAA, CCC and GGG, where A, C and G is adenine, cytosine and guanine, respectively). In phosphate-acetate buffer at pH 5.8 all heptamers were adsorbed on a hanging mercury electrode and further reduced and oxidized. The voltammetric reduction peaks of A and C moiety and oxidation peaks of G moiety at different concentrations (0.25 to 10 µmol•L-1) were analyzed. It was found that the changes in the central trinucleotide sequence of heptamers result in altered adsorption ability at a charged phase interface. An electron transfer process in an adsorptive state was evidenced by the elimination voltammetric procedure (EVP). For the evaluation of adsorption efficiency of heptamers the Langmuir adsorption model was applied and the adsorption coefficient b was calculated.

Links

LH13053, research and development project

Name: Chemická a elektrochemická analýza purinových derivátů a jejich komplexů s mědí (Acronym: CECAP)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1452/2014, interní kód MU

Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2015

Investor: Masaryk University, Category A

Citovat

TŘÍSKOVÁ, Iveta, Libor GURECKÝ and Libuše TRNKOVÁ. Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode. Monatshefte für Chemie - Chemical Monthly. Wien: Springer-Verlag, 2016, vol. 147, No 5, p. 857-864. ISSN 0026-9247. Available from: https://dx.doi.org/10.1007/s00706-016-1689-7.

@article{1348215,
   author = {Třísková, Iveta and Gurecký, Libor and Trnková, Libuše},
   article_location = {Wien},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00706-016-1689-7},
   keywords = {DNA heptamers; hanging mercury drop electrode; linear sweep voltammetry; elimination voltammetric procedure; adsorption isotherm; adsorption coefficient},
   language = {eng},
   issn = {0026-9247},
   journal = {Monatshefte für Chemie - Chemical Monthly},
   title = {Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode},
   url = {http://dx.doi.org/10.1007/s00706-016-1689-7},
   volume = {147},
   year = {2016}
}

TY  - JOUR
ID  - 1348215
AU  - Třísková, Iveta - Gurecký, Libor - Trnková, Libuše
PY  - 2016
TI  - Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode
JF  - Monatshefte für Chemie - Chemical Monthly
VL  - 147
IS  - 5
SP  - 857-864
EP  - 857-864
PB  - Springer-Verlag
SN  - 00269247
KW  - DNA heptamers
KW  - hanging mercury drop electrode
KW  - linear sweep voltammetry
KW  - elimination voltammetric procedure
KW  - adsorption isotherm
KW  - adsorption coefficient
UR  - http://dx.doi.org/10.1007/s00706-016-1689-7
L2  - http://dx.doi.org/10.1007/s00706-016-1689-7
N2  - The focus of the presented study is electrochemical investigation of DNA heptamers d(GCNNNGC) with a different center trinucleotide sequence (NNN = GAA, AAA, CCC and GGG, where A, C and G is adenine, cytosine and guanine, respectively). In phosphate-acetate buffer at pH 5.8 all heptamers were adsorbed on a hanging mercury electrode and further reduced and oxidized. The voltammetric reduction peaks of A and C moiety and oxidation peaks of G moiety at different concentrations (0.25 to 10 µmol•L-1) were analyzed. It was found that the changes in the central trinucleotide sequence of heptamers result in altered adsorption ability at a charged phase interface. An electron transfer process in an adsorptive state was evidenced by the elimination voltammetric procedure (EVP). For the evaluation of adsorption efficiency of heptamers the Langmuir adsorption model was applied and the adsorption coefficient b was calculated.
ER  -

TŘÍSKOVÁ, Iveta, Libor GURECKÝ and Libuše TRNKOVÁ. Switching central trinucleotide sequences of DNA hepatmer regulates adsorption on mercury electrode. \textit{Monatshefte für Chemie - Chemical Monthly}. Wien: Springer-Verlag, 2016, vol.~147, No~5, p.~857-864. ISSN~0026-9247. Available from: https://dx.doi.org/10.1007/s00706-016-1689-7.

Detailed Information on Publication Record