J 2016

Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins

SEPSIOVA, Regina, Ivona NEČASOVÁ, Smaranda WILLCOX, Katarina PROCHAZKOVA, Peter GORILÁK et. al.

Basic information

Original name

Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins

Authors

SEPSIOVA, Regina (703 Slovakia), Ivona NEČASOVÁ (203 Czech Republic, belonging to the institution), Smaranda WILLCOX (840 United States of America), Katarina PROCHAZKOVA (703 Slovakia), Peter GORILÁK (703 Slovakia), Jozef NOSEK (703 Slovakia), Ctirad HOFR (203 Czech Republic, guarantor, belonging to the institution), Jack D. GRIFFITH (840 United States of America) and Ĺubomír TOMÁŠKA (703 Slovakia)

Edition

Plos one, San Francisco, Public Library of Science, 2016, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.806

RIV identification code

RIV/00216224:14740/16:00088004

Organization unit

Central European Institute of Technology

DOI

UT WoS

000374898500167

Keywords in English

MYB-DOMAIN PROTEIN; FISSION YEAST; HUMAN RAP1; USTILAGO-MAYDIS; TAY1 PROTEIN; DNA; TAZ1; TRF2; IDENTIFICATION; SEQUENCE

Tags

Tags

International impact, Reviewed
Změněno: 17/12/2019 14:09, Mgr. Marie Šípková, DiS.

Abstract

V originále

Telomeres of nuclear chromosomes are usually composed of an array of tandemly repeated sequences that are recognized by specific Myb domain containing DNA-binding proteins (telomere-binding proteins, TBPs). Whereas in many eukaryotes the length and sequence of the telomeric repeat is relatively conserved, telomeric sequences in various yeasts are highly variable. Schizosaccharomyces pombe provides an excellent model for investigation of co-evolution of telomeres and TBPs. First, telomeric repeats of S. pombe differ from the canonical mammalian type TTAGGG sequence. Second, S. pombe telomeres exhibit a high degree of intratelomeric heterogeneity. Third, S. pombe contains all types of known TBPs (Rap1p [a version unable to bind DNA], Tay1p/Teb1p, and Taz1p) that are employed by various yeast species to protect their telomeres. With the aim of reconstructing evolutionary paths leading to a separation of roles between Teb1p and Taz1p, we performed a comparative analysis of the DNA-binding properties of both proteins using combined qualitative and quantitative biochemical approaches. Visualization of DNA-protein complexes by electron microscopy revealed qualitative differences of binding of Teb1p and Taz1p to mammalian type and fission yeast telomeres. Fluorescence anisotropy analysis quantified the binding affinity of Teb1p and Taz1p to three different DNA substrates. Additionally, we carried out electrophoretic mobility shift assays using mammalian type telomeres and native substrates (telomeric repeats, histone-box sequences) as well as their mutated versions. We observed relative DNA sequence binding flexibility of Taz1p and higher binding stringency of Teb1p when both proteins were compared directly to each other. These properties may have driven replacement of Teb1p by Taz1p as the TBP in fission yeast.

Links

GAP205/12/0550, research and development project
Name: Dynamika vzniku shelterinového komplexu
Investor: Czech Science Foundation
LH13054, research and development project
Name: Studium dynamiky telomerových proteinových komplexů zajišťujících stabilitu genomu (Acronym: TeloProtGen)
Investor: Ministry of Education, Youth and Sports of the CR