Effect of ethanol at clinically relevant concentrations on
atrial inward rectifier potassium current sensitive to
acetylcholine

J 2016

Effect of ethanol at clinically relevant concentrations on atrial inward rectifier potassium current sensitive to acetylcholine

BÉBAROVÁ, Markéta, Peter MATEJOVIČ, Michal PÁSEK, Zuzana HOŘÁKOVÁ, Jan HOŠEK et. al.

Basic information

Original name

Effect of ethanol at clinically relevant concentrations on atrial inward rectifier potassium current sensitive to acetylcholine

Authors

BÉBAROVÁ, Markéta (203 Czech Republic, guarantor, belonging to the institution), Peter MATEJOVIČ (203 Czech Republic, belonging to the institution), Michal PÁSEK (203 Czech Republic, belonging to the institution), Zuzana HOŘÁKOVÁ (203 Czech Republic, belonging to the institution), Jan HOŠEK (203 Czech Republic, belonging to the institution), Milena ŠIMURDOVÁ (203 Czech Republic, belonging to the institution) and Jiří ŠIMURDA (203 Czech Republic, belonging to the institution)

Edition

Naunyn-Schmiedeberg's Archives of Pharmacology, New York, Springer, 2016, 0028-1298

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.558

RIV identification code

RIV/00216224:14110/16:00088863

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00210-016-1265-z

UT WoS

000383665800002

Keywords in English

Ethanol; Inward rectifier; Dual effect; Kir3.1/3.4; Rat atrial cellmodel

Abstract

V originále

Alcohol intoxication tends to induce arrhythmias, most often the atrial fibrillation. To elucidate arrhythmogenic mechanisms related to alcohol consumption, the effect of ethanol on main components of the ionic membrane current is investigated step by step. Considering limited knowledge, we aimed to examine the effect of clinically relevant concentrations of ethanol (0.8-80 mM) on acetylcholine-sensitive inward rectifier potassium current I (K(Ach)). Experiments were performed by the whole-cell patch clamp technique at 23 +/- 1 A degrees C on isolated rat and guinea-pig atrial myocytes, and on expressed human Kir3.1/3.4 channels. Ethanol induced changes of I (K(Ach)) in the whole range of concentrations applied; the effect was not voltage dependent. The constitutively active component of I (K(Ach)) was significantly increased by ethanol with the maximum effect (an increase by similar to 100 %) between 8 and 20 mM. The changes were comparable in rat and guinea-pig atrial myocytes and also in expressed human Kir3.1/3.4 channels (i.e., structural correlate of I (K(Ach))). In the case of the acetylcholine-induced component of I (K(Ach)), a dual ethanol effect was apparent with a striking heterogeneity of changes in individual cells. The effect correlated with the current magnitude in control: the current was increased by eth-anol in the cells showing small current in control and vice versa. The average effect peaked at 20 mM ethanol (an increase of the current by similar to 20 %). Observed changes of action potential duration agreed well with the voltage clamp data. Ethanol significantly affected both components of I (K(Ach)) even in concentrations corresponding to light alcohol consumption.

Links

NT14301, research and development project

Name: Vliv ethanolu a jeho metabolitu acetaldehydu na srdeční inward rectifier draslíkové proudy: vztah k fibrilaci síní po konzumaci alkoholu?

Investor: Ministry of Health of the CR

Citovat

BÉBAROVÁ, Markéta, Peter MATEJOVIČ, Michal PÁSEK, Zuzana HOŘÁKOVÁ, Jan HOŠEK, Milena ŠIMURDOVÁ and Jiří ŠIMURDA. Effect of ethanol at clinically relevant concentrations on atrial inward rectifier potassium current sensitive to acetylcholine. Naunyn-Schmiedeberg's Archives of Pharmacology. New York: Springer, 2016, vol. 389, No 10, p. 1049-1058. ISSN 0028-1298. Available from: https://dx.doi.org/10.1007/s00210-016-1265-z.

@article{1348898,
   author = {Bébarová, Markéta and Matejovič, Peter and Pásek, Michal and Hořáková, Zuzana and Hošek, Jan and Šimurdová, Milena and Šimurda, Jiří},
   article_location = {New York},
   article_number = {10},
   doi = {http://dx.doi.org/10.1007/s00210-016-1265-z},
   keywords = {Ethanol; Inward rectifier; Dual effect; Kir3.1/3.4; Rat atrial cellmodel},
   language = {eng},
   issn = {0028-1298},
   journal = {Naunyn-Schmiedeberg's Archives of Pharmacology},
   title = {Effect of ethanol at clinically relevant concentrations on atrial inward rectifier potassium current sensitive to acetylcholine},
   volume = {389},
   year = {2016}
}

TY  - JOUR
ID  - 1348898
AU  - Bébarová, Markéta - Matejovič, Peter - Pásek, Michal - Hořáková, Zuzana - Hošek, Jan - Šimurdová, Milena - Šimurda, Jiří
PY  - 2016
TI  - Effect of ethanol at clinically relevant concentrations on atrial inward rectifier potassium current sensitive to acetylcholine
JF  - Naunyn-Schmiedeberg's Archives of Pharmacology
VL  - 389
IS  - 10
SP  - 1049-1058
EP  - 1049-1058
PB  - Springer
SN  - 00281298
KW  - Ethanol
KW  - Inward rectifier
KW  - Dual effect
KW  - Kir3.1/3.4
KW  - Rat atrial cellmodel
N2  - Alcohol intoxication tends to induce arrhythmias, most often the atrial fibrillation. To elucidate arrhythmogenic mechanisms related to alcohol consumption, the effect of ethanol on main components of the ionic membrane current is investigated step by step. Considering limited knowledge, we aimed to examine the effect of clinically relevant concentrations of ethanol (0.8-80 mM) on acetylcholine-sensitive inward rectifier potassium current I (K(Ach)). Experiments were performed by the whole-cell patch clamp technique at 23 +/- 1 A degrees C on isolated rat and guinea-pig atrial myocytes, and on expressed human Kir3.1/3.4 channels. Ethanol induced changes of I (K(Ach)) in the whole range of concentrations applied; the effect was not voltage dependent. The constitutively active component of I (K(Ach)) was significantly increased by ethanol with the maximum effect (an increase by similar to 100 %) between 8 and 20 mM. The changes were comparable in rat and guinea-pig atrial myocytes and also in expressed human Kir3.1/3.4 channels (i.e., structural correlate of I (K(Ach))). In the case of the acetylcholine-induced component of I (K(Ach)), a dual ethanol effect was apparent with a striking heterogeneity of changes in individual cells. The effect correlated with the current magnitude in control: the current was increased by eth-anol in the cells showing small current in control and vice versa. The average effect peaked at 20 mM ethanol (an increase of the current by similar to 20 %). Observed changes of action potential duration agreed well with the voltage clamp data. Ethanol significantly affected both components of I (K(Ach)) even in concentrations corresponding to light alcohol consumption.
ER  -

BÉBAROVÁ, Markéta, Peter MATEJOVIČ, Michal PÁSEK, Zuzana HOŘÁKOVÁ, Jan HOŠEK, Milena ŠIMURDOVÁ and Jiří ŠIMURDA. Effect of ethanol at clinically relevant concentrations on atrial inward rectifier potassium current sensitive to acetylcholine. \textit{Naunyn-Schmiedeberg's Archives of Pharmacology}. New York: Springer, 2016, vol.~389, No~10, p.~1049-1058. ISSN~0028-1298. Available from: https://dx.doi.org/10.1007/s00210-016-1265-z.

Detailed Information on Publication Record