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@article{1349967,
author = {Michalcová, Lenka and Glatz, Zdeněk},
article_location = {Weinheim},
article_number = {18},
doi = {http://dx.doi.org/10.1002/jssc.201600713},
keywords = {Binding constant; Capillary electrophoresis-frontal analysis; Glycated human serum albumin; Human serum albumin; Sulfonylureas antidiabetics;},
language = {eng},
issn = {1615-9306},
journal = {JOURNAL OF SEPARATION SCIENCE},
title = {Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysisis},
volume = {39},
year = {2016}
}
TY - JOUR
ID - 1349967
AU - Michalcová, Lenka - Glatz, Zdeněk
PY - 2016
TI - Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysisis
JF - JOURNAL OF SEPARATION SCIENCE
VL - 39
IS - 18
SP - 3631-3637
EP - 3631-3637
PB - Wiley
SN - 16159306
KW - Binding constant
KW - Capillary electrophoresis-frontal analysis
KW - Glycated human serum albumin
KW - Human serum albumin
KW - Sulfonylureas antidiabetics;
N2 - Diabetes is one of the most widespread diseases characterised by a deficiency in the production of insulin, or its ineffectiveness. As a result, the increased concentrations of glucose in the blood lead not only to damage to many of the body's systems but also causes the non-enzymatic glycation of plasma proteins affecting their drug binding. Since the binding ability influences its pharmacokinetics and pharmacodynamics, this is a very important issue in the development of new drugs and personalised medicine. In this study, capillary electrophoresis-frontal analysis was used to evaluate the affinities between human serum albumin or its glycated form and the first generation of sulfonylurea antidiabetics, since their inadequate concentration may induce hypoglycaemia or on the contrary hyperglycaemia. The binding constants decrease in the sequence acetohexamide > tolbutamide > chlorpropamide > carbutamide both for normal and glycated human serum albumins, with glycated giving lower values. These results provide a more quantitative picture of how these drugs bind with normal and modified human serum albumin and indicate capillary electrophoresis frontal analysis to be another tool for examining the changes arising from modifications of albumin, or any other protein, with all its benefits like short analysis time, small sample requirement and automation.
ER -
MICHALCOVÁ, Lenka and Zdeněk GLATZ. Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysisis (Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysis). \textit{JOURNAL OF SEPARATION SCIENCE}. Weinheim: Wiley, 2016, vol.~39, No~18, p.~3631-3637. ISSN~1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201600713.
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