Study on the interactions of sulfonylurea antidiabetic drugs
with normal and glycated human serum albumin by capillary
electrophoresis-frontal analysisis

J 2016

Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysisis

MICHALCOVÁ, Lenka and Zdeněk GLATZ

Basic information

Original name

Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysisis

Name in Czech

Byla provedena studie interakcí normálního a glykovaného HSA s antydiabetickými léčivy pomocí CE-FA

Authors

MICHALCOVÁ, Lenka (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF SEPARATION SCIENCE, Weinheim, Wiley, 2016, 1615-9306

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.557

RIV identification code

RIV/00216224:14310/16:00088036

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/jssc.201600713

UT WoS

000384667800020

Keywords (in Czech)

Vazebná konstanta; Capilární electrophoresa-frontalní analysýz; Glykovaný HSA; Normální HSA; Sulfonylurylová antidiabetika;

Keywords in English

Binding constant; Capillary electrophoresis-frontal analysis; Glycated human serum albumin; Human serum albumin; Sulfonylureas antidiabetics;

Abstract

ORIG CZ

V originále

Diabetes is one of the most widespread diseases characterised by a deficiency in the production of insulin, or its ineffectiveness. As a result, the increased concentrations of glucose in the blood lead not only to damage to many of the body's systems but also causes the non-enzymatic glycation of plasma proteins affecting their drug binding. Since the binding ability influences its pharmacokinetics and pharmacodynamics, this is a very important issue in the development of new drugs and personalised medicine. In this study, capillary electrophoresis-frontal analysis was used to evaluate the affinities between human serum albumin or its glycated form and the first generation of sulfonylurea antidiabetics, since their inadequate concentration may induce hypoglycaemia or on the contrary hyperglycaemia. The binding constants decrease in the sequence acetohexamide > tolbutamide > chlorpropamide > carbutamide both for normal and glycated human serum albumins, with glycated giving lower values. These results provide a more quantitative picture of how these drugs bind with normal and modified human serum albumin and indicate capillary electrophoresis frontal analysis to be another tool for examining the changes arising from modifications of albumin, or any other protein, with all its benefits like short analysis time, small sample requirement and automation.

In Czech

Byla provedena studie interakcí normálního a glykovaného HSA s antydiabetickými léčivy pomocí CE-FA

Links

GBP206/12/G014, research and development project

Name: Centrum pokročilých bioanalytických technologií

MUNI/A/1265/2015, interní kód MU

Name: Podpora biochemického výzkumu v roce 2016

Investor: Masaryk University, Category A

Citovat

MICHALCOVÁ, Lenka and Zdeněk GLATZ. Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysisis (Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysis). JOURNAL OF SEPARATION SCIENCE. Weinheim: Wiley, 2016, vol. 39, No 18, p. 3631-3637. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201600713.

@article{1349967,
   author = {Michalcová, Lenka and Glatz, Zdeněk},
   article_location = {Weinheim},
   article_number = {18},
   doi = {http://dx.doi.org/10.1002/jssc.201600713},
   keywords = {Binding constant; Capillary electrophoresis-frontal analysis; Glycated human serum albumin; Human serum albumin; Sulfonylureas antidiabetics;},
   language = {eng},
   issn = {1615-9306},
   journal = {JOURNAL OF SEPARATION SCIENCE},
   title = {Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysisis},
   volume = {39},
   year = {2016}
}

TY  - JOUR
ID  - 1349967
AU  - Michalcová, Lenka - Glatz, Zdeněk
PY  - 2016
TI  - Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysisis
JF  - JOURNAL OF SEPARATION SCIENCE
VL  - 39
IS  - 18
SP  - 3631-3637
EP  - 3631-3637
PB  - Wiley
SN  - 16159306
KW  - Binding constant
KW  - Capillary electrophoresis-frontal analysis
KW  - Glycated human serum albumin
KW  - Human serum albumin
KW  - Sulfonylureas antidiabetics;
N2  - Diabetes is one of the most widespread diseases characterised by a deficiency in the production of insulin, or its ineffectiveness. As a result, the increased concentrations of glucose in the blood lead not only to damage to many of the body's systems but also causes the non-enzymatic glycation of plasma proteins affecting their drug binding. Since the binding ability influences its pharmacokinetics and pharmacodynamics, this is a very important issue in the development of new drugs and personalised medicine. In this study, capillary electrophoresis-frontal analysis was used to evaluate the affinities between human serum albumin or its glycated form and the first generation of sulfonylurea antidiabetics, since their inadequate concentration may induce hypoglycaemia or on the contrary hyperglycaemia. The binding constants decrease in the sequence acetohexamide > tolbutamide > chlorpropamide > carbutamide both for normal and glycated human serum albumins, with glycated giving lower values. These results provide a more quantitative picture of how these drugs bind with normal and modified human serum albumin and indicate capillary electrophoresis frontal analysis to be another tool for examining the changes arising from modifications of albumin, or any other protein, with all its benefits like short analysis time, small sample requirement and automation.
ER  -

MICHALCOVÁ, Lenka and Zdeněk GLATZ. Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysisis (Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis-frontal analysis). \textit{JOURNAL OF SEPARATION SCIENCE}. Weinheim: Wiley, 2016, vol.~39, No~18, p.~3631-3637. ISSN~1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201600713.

Detailed Information on Publication Record