Detection of selected periodontal bacteria in preschool
children affected by early childhood caries

J 2016

Detection of selected periodontal bacteria in preschool children affected by early childhood caries

PANTUČKOVÁ, Pavla, Michaela BARTOŠOVÁ, Zdeněk BROUKAL, Martina KUKLETOVÁ, Lydie IZAKOVIČOVÁ HOLLÁ et. al.

Basic information

Original name

Detection of selected periodontal bacteria in preschool children affected by early childhood caries

Name in Czech

Detekce vybraných parodontálních bakterií u malých dětí postižených kazem raného dětství

Authors

PANTUČKOVÁ, Pavla (203 Czech Republic, belonging to the institution), Michaela BARTOŠOVÁ (703 Slovakia, belonging to the institution), Zdeněk BROUKAL (203 Czech Republic), Martina KUKLETOVÁ (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Folia Microbiologica, Dordrecht, Springer, 2016, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.521

RIV identification code

RIV/00216224:14110/16:00088043

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s12223-016-0468-5

UT WoS

000386351700012

Keywords in English

PORPHYROMONAS-GINGIVALIS; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; SUBGINGIVAL MICROBIOME; HEALTHY-CHILDREN; MIXED DENTITION; ORAL MICROBIOTA; YOUNG-CHILDREN; PRIMARY TEETH; COLONIZATION; PATHOGENS

Abstract

ORIG CZ

V originále

The aim of this study was to compare the detection frequency of periodontal bacteria in dental plaque in children with early childhood caries (ECC) with and without gingival inflammation. A convenience sample of 25 preschool children (mean age 3.61 years, SD 1.42) was recruited. Dental plaque was taken from periodontal areas with and without visible signs of inflammation and processed using the StomaGeneA (R) (Protean s.r.o. Czech Republic) and ParoCheckA (R) 20 (Greiner Bio-one GmbH, Germany) detection kits. The two sample t tests between percents for differences between inflammatory and healthy sites and kappa statistics for the agreement of both systems were used. At the inflammatory sites, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were significantly more frequently detected by StomaGeneA (R) while Fusobacterium nucleatum, A. actinomycetemcomitans, Tanarella forsythia and Prevotella intermedia were significantly more frequently identified by ParoCheckA (R) test. The agreement between the two detection systems was substantial for A. actinomycetemcomitans and F. nucleatum in the samples collected from inflamed sites and only for F. nucleatum from clinically healthy sites. Therefore, we recommend that the same system should be used when the same patient is examined repeatedly.

In Czech

Cílem této práce bylo porovnat detekci výskytu parodontálních bakterií v zubním plaku u dětí s kazem raného dětství s a bez známek zánětu dásně.

Links

GB14-37368G, research and development project

Name: Centrum orofaciálního vývoje a regenerace

Investor: Czech Science Foundation

MUNI/A/1258/2015, interní kód MU

Name: Analýza klinických, mikrobiálních a genetických faktorů v etiopatogenezi vybraných patologických stavů v dutině ústní

Investor: Masaryk University, Category A

Citovat

PANTUČKOVÁ, Pavla, Michaela BARTOŠOVÁ, Zdeněk BROUKAL, Martina KUKLETOVÁ and Lydie IZAKOVIČOVÁ HOLLÁ. Detection of selected periodontal bacteria in preschool children affected by early childhood caries. Folia Microbiologica. Dordrecht: Springer, 2016, vol. 61, No 6, p. 533-538. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-016-0468-5.

@article{1350437,
   author = {Pantučková, Pavla and Bartošová, Michaela and Broukal, Zdeněk and Kukletová, Martina and Izakovičová Hollá, Lydie},
   article_location = {Dordrecht},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s12223-016-0468-5},
   keywords = {PORPHYROMONAS-GINGIVALIS; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; SUBGINGIVAL MICROBIOME; HEALTHY-CHILDREN; MIXED DENTITION; ORAL MICROBIOTA; YOUNG-CHILDREN; PRIMARY TEETH; COLONIZATION; PATHOGENS},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Detection of selected periodontal bacteria in preschool children affected by early childhood caries},
   volume = {61},
   year = {2016}
}

TY  - JOUR
ID  - 1350437
AU  - Pantučková, Pavla - Bartošová, Michaela - Broukal, Zdeněk - Kukletová, Martina - Izakovičová Hollá, Lydie
PY  - 2016
TI  - Detection of selected periodontal bacteria in preschool children affected by early childhood caries
JF  - Folia Microbiologica
VL  - 61
IS  - 6
SP  - 533-538
EP  - 533-538
PB  - Springer
SN  - 00155632
KW  - PORPHYROMONAS-GINGIVALIS
KW  - ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
KW  - SUBGINGIVAL MICROBIOME
KW  - HEALTHY-CHILDREN
KW  - MIXED DENTITION
KW  - ORAL MICROBIOTA
KW  - YOUNG-CHILDREN
KW  - PRIMARY TEETH
KW  - COLONIZATION
KW  - PATHOGENS
N2  - The aim of this study was to compare the detection frequency of periodontal bacteria in dental plaque in children with early childhood caries (ECC) with and without gingival inflammation. A convenience sample of 25 preschool children (mean age 3.61 years, SD 1.42) was recruited. Dental plaque was taken from periodontal areas with and without visible signs of inflammation and processed using the StomaGeneA (R) (Protean s.r.o. Czech Republic) and ParoCheckA (R) 20 (Greiner Bio-one GmbH, Germany) detection kits. The two sample t tests between percents for differences between inflammatory and healthy sites and kappa statistics for the agreement of both systems were used. At the inflammatory sites, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were significantly more frequently detected by StomaGeneA (R) while Fusobacterium nucleatum, A. actinomycetemcomitans, Tanarella forsythia and Prevotella intermedia were significantly more frequently identified by ParoCheckA (R) test. The agreement between the two detection systems was substantial for A. actinomycetemcomitans and F. nucleatum in the samples collected from inflamed sites and only for F. nucleatum from clinically healthy sites. Therefore, we recommend that the same system should be used when the same patient is examined repeatedly.
ER  -

PANTUČKOVÁ, Pavla, Michaela BARTOŠOVÁ, Zdeněk BROUKAL, Martina KUKLETOVÁ and Lydie IZAKOVIČOVÁ HOLLÁ. Detection of selected periodontal bacteria in preschool children affected by early childhood caries. \textit{Folia Microbiologica}. Dordrecht: Springer, 2016, vol.~61, No~6, p.~533-538. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-016-0468-5.

Detailed Information on Publication Record