PANTUČKOVÁ, Pavla, Michaela BARTOŠOVÁ, Zdeněk BROUKAL, Martina KUKLETOVÁ and Lydie IZAKOVIČOVÁ HOLLÁ. Detection of selected periodontal bacteria in preschool children affected by early childhood caries. Folia Microbiologica. Dordrecht: Springer, 2016, vol. 61, No 6, p. 533-538. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-016-0468-5.
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Basic information
Original name Detection of selected periodontal bacteria in preschool children affected by early childhood caries
Name in Czech Detekce vybraných parodontálních bakterií u malých dětí postižených kazem raného dětství
Authors PANTUČKOVÁ, Pavla (203 Czech Republic, belonging to the institution), Michaela BARTOŠOVÁ (703 Slovakia, belonging to the institution), Zdeněk BROUKAL (203 Czech Republic), Martina KUKLETOVÁ (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition Folia Microbiologica, Dordrecht, Springer, 2016, 0015-5632.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30200 3.2 Clinical medicine
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.521
RIV identification code RIV/00216224:14110/16:00088043
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1007/s12223-016-0468-5
UT WoS 000386351700012
Keywords in English PORPHYROMONAS-GINGIVALIS; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; SUBGINGIVAL MICROBIOME; HEALTHY-CHILDREN; MIXED DENTITION; ORAL MICROBIOTA; YOUNG-CHILDREN; PRIMARY TEETH; COLONIZATION; PATHOGENS
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 23/11/2016 14:03.
Abstract
The aim of this study was to compare the detection frequency of periodontal bacteria in dental plaque in children with early childhood caries (ECC) with and without gingival inflammation. A convenience sample of 25 preschool children (mean age 3.61 years, SD 1.42) was recruited. Dental plaque was taken from periodontal areas with and without visible signs of inflammation and processed using the StomaGeneA (R) (Protean s.r.o. Czech Republic) and ParoCheckA (R) 20 (Greiner Bio-one GmbH, Germany) detection kits. The two sample t tests between percents for differences between inflammatory and healthy sites and kappa statistics for the agreement of both systems were used. At the inflammatory sites, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were significantly more frequently detected by StomaGeneA (R) while Fusobacterium nucleatum, A. actinomycetemcomitans, Tanarella forsythia and Prevotella intermedia were significantly more frequently identified by ParoCheckA (R) test. The agreement between the two detection systems was substantial for A. actinomycetemcomitans and F. nucleatum in the samples collected from inflamed sites and only for F. nucleatum from clinically healthy sites. Therefore, we recommend that the same system should be used when the same patient is examined repeatedly.
Abstract (in Czech)
Cílem této práce bylo porovnat detekci výskytu parodontálních bakterií v zubním plaku u dětí s kazem raného dětství s a bez známek zánětu dásně.
Links
GB14-37368G, research and development projectName: Centrum orofaciálního vývoje a regenerace
Investor: Czech Science Foundation
MUNI/A/1258/2015, interní kód MUName: Analýza klinických, mikrobiálních a genetických faktorů v etiopatogenezi vybraných patologických stavů v dutině ústní
Investor: Masaryk University, Category A
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