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@article{1350497,
author = {Franek, Michal and Suchánková, Jana and ŘezníčkováandPodloučková, Petra and Krejčí, Jana and Legartová, Soňa and Kozubek, Stanislav and Večeřa, J and Sorokin, Dmitry and Bártová, Eva},
article_location = {Saarbrücken},
article_number = {2},
doi = {http://dx.doi.org/10.1017/S1431927616000052},
keywords = {Live-cell studies; microscopy; fluorescent proteins; chromatin; tissue sections},
language = {eng},
issn = {1431-9276},
journal = {Microscopy and Microanalysis},
title = {Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections},
url = {http://dx.doi.org/10.1017/S1431927616000052},
volume = {22},
year = {2016}
}
TY - JOUR
ID - 1350497
AU - Franek, Michal - Suchánková, Jana - Řezníčková-Podloučková, Petra - Krejčí, Jana - Legartová, Soňa - Kozubek, Stanislav - Večeřa, J - Sorokin, Dmitry - Bártová, Eva
PY - 2016
TI - Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections
JF - Microscopy and Microanalysis
VL - 22
IS - 2
SP - 326-341
EP - 326-341
PB - Cambridge University Press
SN - 14319276
KW - Live-cell studies
KW - microscopy
KW - fluorescent proteins
KW - chromatin
KW - tissue sections
UR - http://dx.doi.org/10.1017/S1431927616000052
L2 - http://dx.doi.org/10.1017/S1431927616000052
N2 - Studies on fixed samples or genome-wide analyses of nuclear processes are useful for generating snapshots of a cell population at a particular time point. However, these experimental approaches do not provide information at the single-cell level. Genome-wide studies cannot assess variability between individual cells that are cultured in vitro or originate from different pathological stages. Immunohistochemistry and immunofluorescence are fundamental experimental approaches in clinical laboratories and are also widely used in basic research. However, the fixation procedure may generate artifacts and prevents monitoring of the dynamics of nuclear processes. Therefore, live-cell imaging is critical for studying the kinetics of basic nuclear events, such as DNA replication, transcription, splicing, and DNA repair. This review is focused on the advanced microscopy analyses of the cells, with a particular focus on live cells. We note some methodological innovations and new options for microscope systems that can also be used to study tissue sections. Cornerstone methods for the biophysical research of living cells, such as fluorescence recovery after photobleaching and fluorescence resonance energy transfer, are also discussed, as are studies on the effects of radiation at the individual cellular level.
ER -
FRANEK, Michal, Jana SUCHÁNKOVÁ, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Jana KREJČÍ, Soňa LEGARTOVÁ, Stanislav KOZUBEK, J VEČEŘA, Dmitry SOROKIN a Eva BÁRTOVÁ. Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections. Online. \textit{Microscopy and Microanalysis}. Saarbrücken: Cambridge University Press, 2016, roč.~22, č.~2, s.~326-341. ISSN~1431-9276. Dostupné z: https://dx.doi.org/10.1017/S1431927616000052. [citováno 2024-04-24]
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