Advanced Image Acquisition and Analytical Techniques for
Studies of Living Cells and Tissue Sections

J 2016

Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections

FRANEK, Michal, Jana SUCHÁNKOVÁ, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Jana KREJČÍ, Soňa LEGARTOVÁ et. al.

Basic information

Original name

Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections

Authors

FRANEK, Michal, Jana SUCHÁNKOVÁ, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Jana KREJČÍ, Soňa LEGARTOVÁ, Stanislav KOZUBEK, J VEČEŘA, Dmitry SOROKIN and Eva BÁRTOVÁ

Edition

Microscopy and Microanalysis, Saarbrücken, Cambridge University Press, 2016, 1431-9276

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.891

DOI

http://dx.doi.org/10.1017/S1431927616000052

UT WoS

000378274600008

Keywords in English

Live-cell studies; microscopy; fluorescent proteins; chromatin; tissue sections

Abstract

V originále

Studies on fixed samples or genome-wide analyses of nuclear processes are useful for generating snapshots of a cell population at a particular time point. However, these experimental approaches do not provide information at the single-cell level. Genome-wide studies cannot assess variability between individual cells that are cultured in vitro or originate from different pathological stages. Immunohistochemistry and immunofluorescence are fundamental experimental approaches in clinical laboratories and are also widely used in basic research. However, the fixation procedure may generate artifacts and prevents monitoring of the dynamics of nuclear processes. Therefore, live-cell imaging is critical for studying the kinetics of basic nuclear events, such as DNA replication, transcription, splicing, and DNA repair. This review is focused on the advanced microscopy analyses of the cells, with a particular focus on live cells. We note some methodological innovations and new options for microscope systems that can also be used to study tissue sections. Cornerstone methods for the biophysical research of living cells, such as fluorescence recovery after photobleaching and fluorescence resonance energy transfer, are also discussed, as are studies on the effects of radiation at the individual cellular level.

Citovat

FRANEK, Michal, Jana SUCHÁNKOVÁ, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Jana KREJČÍ, Soňa LEGARTOVÁ, Stanislav KOZUBEK, J VEČEŘA, Dmitry SOROKIN and Eva BÁRTOVÁ. Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections. Microscopy and Microanalysis. Saarbrücken: Cambridge University Press, 2016, vol. 22, No 2, p. 326-341. ISSN 1431-9276. Available from: https://dx.doi.org/10.1017/S1431927616000052.

@article{1350497,
   author = {Franek, Michal and Suchánková, Jana and ŘezníčkováandPodloučková, Petra and Krejčí, Jana and Legartová, Soňa and Kozubek, Stanislav and Večeřa, J and Sorokin, Dmitry and Bártová, Eva},
   article_location = {Saarbrücken},
   article_number = {2},
   doi = {http://dx.doi.org/10.1017/S1431927616000052},
   keywords = {Live-cell studies; microscopy; fluorescent proteins; chromatin; tissue sections},
   language = {eng},
   issn = {1431-9276},
   journal = {Microscopy and Microanalysis},
   title = {Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections},
   url = {http://dx.doi.org/10.1017/S1431927616000052},
   volume = {22},
   year = {2016}
}

TY  - JOUR
ID  - 1350497
AU  - Franek, Michal - Suchánková, Jana - Řezníčková-Podloučková, Petra - Krejčí, Jana - Legartová, Soňa - Kozubek, Stanislav - Večeřa, J - Sorokin, Dmitry - Bártová, Eva
PY  - 2016
TI  - Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections
JF  - Microscopy and Microanalysis
VL  - 22
IS  - 2
SP  - 326-341
EP  - 326-341
PB  - Cambridge University Press
SN  - 14319276
KW  - Live-cell studies
KW  - microscopy
KW  - fluorescent proteins
KW  - chromatin
KW  - tissue sections
UR  - http://dx.doi.org/10.1017/S1431927616000052
L2  - http://dx.doi.org/10.1017/S1431927616000052
N2  - Studies on fixed samples or genome-wide analyses of nuclear processes are useful for generating snapshots of a cell population at a particular time point. However, these experimental approaches do not provide information at the single-cell level. Genome-wide studies cannot assess variability between individual cells that are cultured in vitro or originate from different pathological stages. Immunohistochemistry and immunofluorescence are fundamental experimental approaches in clinical laboratories and are also widely used in basic research. However, the fixation procedure may generate artifacts and prevents monitoring of the dynamics of nuclear processes. Therefore, live-cell imaging is critical for studying the kinetics of basic nuclear events, such as DNA replication, transcription, splicing, and DNA repair. This review is focused on the advanced microscopy analyses of the cells, with a particular focus on live cells. We note some methodological innovations and new options for microscope systems that can also be used to study tissue sections. Cornerstone methods for the biophysical research of living cells, such as fluorescence recovery after photobleaching and fluorescence resonance energy transfer, are also discussed, as are studies on the effects of radiation at the individual cellular level.
ER  -

FRANEK, Michal, Jana SUCHÁNKOVÁ, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Jana KREJČÍ, Soňa LEGARTOVÁ, Stanislav KOZUBEK, J VEČEŘA, Dmitry SOROKIN and Eva BÁRTOVÁ. Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections. \textit{Microscopy and Microanalysis}. Saarbrücken: Cambridge University Press, 2016, vol.~22, No~2, p.~326-341. ISSN~1431-9276. Available from: https://dx.doi.org/10.1017/S1431927616000052.

Detailed Information on Publication Record