Transgelin is upregulated in stromal cells of lymph node
positive breast cancer

J 2016

Transgelin is upregulated in stromal cells of lymph node positive breast cancer

DVOŘÁKOVÁ, Monika, Jarmila SOBOTKOVÁ, Iva PROCHÁZKOVÁ, Juraj LENČO, Rudolf NENUTIL et. al.

Basic information

Original name

Transgelin is upregulated in stromal cells of lymph node positive breast cancer

Authors

DVOŘÁKOVÁ, Monika (203 Czech Republic, belonging to the institution), Jarmila SOBOTKOVÁ (203 Czech Republic, belonging to the institution), Iva PROCHÁZKOVÁ (203 Czech Republic), Juraj LENČO (203 Czech Republic), Rudolf NENUTIL (203 Czech Republic) and Pavel BOUCHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Proteomics, Amsterdam, ELSEVIER SCIENCE BV, 2016, 1874-3919

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.914

RIV identification code

RIV/00216224:14310/16:00088069

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.jprot.2015.11.025

UT WoS

000368747400011

Keywords in English

Breast cancer; Lymph node metastasis; Transgelin; Transgelin-2; Proteomics

Abstract

V originále

Transgelin and transgelin-2 have been discussed as potential markers of various cancers. Here we identified increased transgelin level in lymph node positive vs. negative, low grade primary breast cancer tissues using 2-DE in the cohort of 12 patients.Wefurther clinically validated 2-DE results in an independent cohort of 48 lowgrade breast cancer patients through untargeted and targeted proteomics analysis (iTRAQ-2D-LC–MS/MS, mTRAQSRM), at transcript level and using immunohistochemistry. Another group of 48 high grade tumors of different breast cancer subtypes was analyzed together with the low grade samples to test transgelin specificity for low grade tumors and to study transgelin relation to known molecular markers and histological features. The results confirmed transgelin connection with the lymph node metastasis. As a marker of a reactive tumor stroma, transgelin can be connected with the higher risk of metastasis development. Moreover, we observed significant down-regulation of transgelin in high vs. low grade tumors caused by decreased content of stromal cells (mainly expressing transgelin) in high grade tumor tissue. We also analyzed expression of transgelin-2 in the second cohort using proteomics and immunohistochemistry. Transgelin-2 was mainly expressed by epithelial cancer cells and its levels were increased in metastatic and poorly differentiated tumors.

Links

GA14-19250S, research and development project

Name: Nový panel proteinů korelujících se stavem lymfatických uzlin u low-grade nádorů prsu: Klinická verifikace a úloha v invazivitě nádorových buněk

Investor: Czech Science Foundation

90004, large research infrastructures

Name: BBMRI-CZ

Citovat

DVOŘÁKOVÁ, Monika, Jarmila SOBOTKOVÁ, Iva PROCHÁZKOVÁ, Juraj LENČO, Rudolf NENUTIL and Pavel BOUCHAL. Transgelin is upregulated in stromal cells of lymph node positive breast cancer. Journal of Proteomics. Amsterdam: ELSEVIER SCIENCE BV, 2016, vol. 132, January, p. 103-111. ISSN 1874-3919. Available from: https://dx.doi.org/10.1016/j.jprot.2015.11.025.

@article{1350932,
   author = {Dvořáková, Monika and Sobotková, Jarmila and Procházková, Iva and Lenčo, Juraj and Nenutil, Rudolf and Bouchal, Pavel},
   article_location = {Amsterdam},
   article_number = {January},
   doi = {http://dx.doi.org/10.1016/j.jprot.2015.11.025},
   keywords = {Breast cancer; Lymph node metastasis; Transgelin; Transgelin-2; Proteomics},
   language = {eng},
   issn = {1874-3919},
   journal = {Journal of Proteomics},
   title = {Transgelin is upregulated in stromal cells of lymph node positive breast cancer},
   url = {https://www.sciencedirect.com/science/article/pii/S1874391915301986?via%3Dihub},
   volume = {132},
   year = {2016}
}

TY  - JOUR
ID  - 1350932
AU  - Dvořáková, Monika - Sobotková, Jarmila - Procházková, Iva - Lenčo, Juraj - Nenutil, Rudolf - Bouchal, Pavel
PY  - 2016
TI  - Transgelin is upregulated in stromal cells of lymph node positive breast cancer
JF  - Journal of Proteomics
VL  - 132
IS  - January
SP  - 103-111
EP  - 103-111
PB  - ELSEVIER SCIENCE BV
SN  - 18743919
KW  - Breast cancer
KW  - Lymph node metastasis
KW  - Transgelin
KW  - Transgelin-2
KW  - Proteomics
UR  - https://www.sciencedirect.com/science/article/pii/S1874391915301986?via%3Dihub
N2  - Transgelin and transgelin-2 have been discussed as potential markers of various cancers. Here we identified increased transgelin level in lymph node positive vs. negative, low grade primary breast cancer tissues using 2-DE in the cohort of 12 patients.Wefurther clinically validated 2-DE results in an independent cohort of 48 lowgrade breast cancer patients through untargeted and targeted proteomics analysis (iTRAQ-2D-LC–MS/MS, mTRAQSRM), at transcript level and using immunohistochemistry. Another group of 48 high grade tumors of different breast cancer subtypes was analyzed together with the low grade samples to test transgelin specificity for low grade tumors and to study transgelin relation to known molecular markers and histological features. The results confirmed transgelin connection with the lymph node metastasis. As a marker of a reactive tumor stroma, transgelin can be connected with the higher risk of metastasis development. Moreover, we observed significant down-regulation of transgelin in high vs. low grade tumors caused by decreased content of stromal cells (mainly expressing transgelin) in high grade tumor tissue. We also analyzed expression of transgelin-2 in the second cohort using proteomics and immunohistochemistry. Transgelin-2 was mainly expressed by epithelial cancer cells and its levels were increased in metastatic and poorly differentiated tumors.
ER  -

DVOŘÁKOVÁ, Monika, Jarmila SOBOTKOVÁ, Iva PROCHÁZKOVÁ, Juraj LENČO, Rudolf NENUTIL and Pavel BOUCHAL. Transgelin is upregulated in stromal cells of lymph node positive breast cancer. \textit{Journal of Proteomics}. Amsterdam: ELSEVIER SCIENCE BV, 2016, vol.~132, January, p.~103-111. ISSN~1874-3919. Available from: https://dx.doi.org/10.1016/j.jprot.2015.11.025.

Detailed Information on Publication Record