Influence of serum albumin on intracellular delivery of
drug-loaded hyaluronan polymeric micelles

J 2016

Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles

NEŠPOROVÁ, Kristina, Jana SOGORKOVA, Daniela SMEJKALOVÁ, J. KULHANEK, G. HUERTA-ANGELES et. al.

Basic information

Original name

Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles

Authors

NEŠPOROVÁ, Kristina (203 Czech Republic, belonging to the institution), Jana SOGORKOVA (203 Czech Republic), Daniela SMEJKALOVÁ (203 Czech Republic, guarantor), J. KULHANEK (203 Czech Republic), G. HUERTA-ANGELES (484 Mexico), Lukáš KUBALA (203 Czech Republic, belonging to the institution) and Vladimír VELEBNÝ (203 Czech Republic)

Edition

International Journal of Pharmaceutics, AMSTERDAM, ELSEVIER SCIENCE BV, 2016, 0378-5173

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.649

RIV identification code

RIV/00216224:14310/16:00095864

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ijpharm.2016.07.057

UT WoS

000382263700065

Keywords in English

Polymeric micelle; Hyaluronan; Fatty acid; Albumin; Cell uptake; Stability

Abstract

V originále

Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs. Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up. Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin. The composition of micelles affected mainly the loading capacity. Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component. This destabilization was found to be influenced by polymeric micelle concentration. Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers. (C) 2016 Published by Elsevier B.V.

Citovat

NEŠPOROVÁ, Kristina, Jana SOGORKOVA, Daniela SMEJKALOVÁ, J. KULHANEK, G. HUERTA-ANGELES, Lukáš KUBALA and Vladimír VELEBNÝ. Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles. International Journal of Pharmaceutics. AMSTERDAM: ELSEVIER SCIENCE BV, 2016, vol. 511, No 1, p. 638-647. ISSN 0378-5173. Available from: https://dx.doi.org/10.1016/j.ijpharm.2016.07.057.

@article{1350938,
   author = {Nešporová, Kristina and Sogorkova, Jana and Smejkalová, Daniela and Kulhanek, J. and HuertaandAngeles, G. and Kubala, Lukáš and Velebný, Vladimír},
   article_location = {AMSTERDAM},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.ijpharm.2016.07.057},
   keywords = {Polymeric micelle; Hyaluronan; Fatty acid; Albumin; Cell uptake; Stability},
   language = {eng},
   issn = {0378-5173},
   journal = {International Journal of Pharmaceutics},
   title = {Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles},
   volume = {511},
   year = {2016}
}

TY  - JOUR
ID  - 1350938
AU  - Nešporová, Kristina - Sogorkova, Jana - Smejkalová, Daniela - Kulhanek, J. - Huerta-Angeles, G. - Kubala, Lukáš - Velebný, Vladimír
PY  - 2016
TI  - Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles
JF  - International Journal of Pharmaceutics
VL  - 511
IS  - 1
SP  - 638-647
EP  - 638-647
PB  - ELSEVIER SCIENCE BV
SN  - 03785173
KW  - Polymeric micelle
KW  - Hyaluronan
KW  - Fatty acid
KW  - Albumin
KW  - Cell uptake
KW  - Stability
N2  - Polymeric micelles are attractive drug delivery systems for intravenously administered nonpolar drugs. Although physical parameters like size, shape and loading capacity are considered as the most important for their efficiency, here we demonstrate that the effects of serum protein interaction and characteristics of loaded compound cannot be neglected during the micelle development and design of experimental set up. Polymeric micelles prepared from amphiphilic hyaluronic acid grafted with short (hexanoic) and long fatty acids (oleic) were tested after loading with two different hydrophobic models, Nile red and curcumin. The composition of micelles affected mainly the loading capacity. Both encapsulated compounds behaved differently in the in vitro cell uptake, which was also influenced by serum concentration, where serum albumin was found to be the primary destabilizing component. This destabilization was found to be influenced by polymeric micelle concentration. Thus, the chemical structure of micelle, the properties of non-covalently loaded substance and serum albumin/polymeric micelle ratio modulate the in vitro intracellular uptake of drugs loaded in nanocarriers. (C) 2016 Published by Elsevier B.V.
ER  -

NEŠPOROVÁ, Kristina, Jana SOGORKOVA, Daniela SMEJKALOVÁ, J. KULHANEK, G. HUERTA-ANGELES, Lukáš KUBALA and Vladimír VELEBNÝ. Influence of serum albumin on intracellular delivery of drug-loaded hyaluronan polymeric micelles. \textit{International Journal of Pharmaceutics}. AMSTERDAM: ELSEVIER SCIENCE BV, 2016, vol.~511, No~1, p.~638-647. ISSN~0378-5173. Available from: https://dx.doi.org/10.1016/j.ijpharm.2016.07.057.

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