Serum-Based MicroRNA Signatures in Early Diagnosis and
Prognosis Prediction of Colon Cancer

J 2016

Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer

VYCHYTILOVÁ, Petra, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka KOSAŘOVÁ, Kateřina SLABÁ et. al.

Basic information

Original name

Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer

Authors

VYCHYTILOVÁ, Petra (203 Czech Republic, belonging to the institution), Lenka RADOVÁ (203 Czech Republic, belonging to the institution), Milana ŠACHLOVÁ (203 Czech Republic, belonging to the institution), Zdeňka KOSAŘOVÁ (203 Czech Republic, belonging to the institution), Kateřina SLABÁ (203 Czech Republic, belonging to the institution), Pavel FABIAN (203 Czech Republic, belonging to the institution), Tomáš GROLICH (203 Czech Republic, belonging to the institution), Vladimír PROCHÁZKA (203 Czech Republic, belonging to the institution), Zdeněk KALA (203 Czech Republic, belonging to the institution), Marek SVOBODA (203 Czech Republic, belonging to the institution), Igor KISS (203 Czech Republic, belonging to the institution), Rostislav VYZULA (203 Czech Republic, belonging to the institution) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Carcinogenesis, Oxford, Oxford University Press, 2016, 0143-3334

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.105

RIV identification code

RIV/00216224:14740/16:00088870

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/carcin/bgw078

UT WoS

000386037100003

Keywords (in Czech)

mikroRNA; kolorektální karcinom; krevní sérum; diagnostický biomarker; prognostický biomarker

Keywords in English

microRNA; colorectal cancer; blood serum; diagnostic biomarker; prognostic biomarker

Abstract

V originále

Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of the present study was to identify serum microRNAs enabling early diagnosis and prognosis prediction of colon cancer. In total, serum samples from 427 colon cancer patients and 276 healthy donors were included in 3-phase biomarker study. Large-scale microRNA expression profiling was performed using Illumina small RNA sequencing. Diagnostic and prognostic potential of identified microRNAs was validated on independent training and validation sets of samples using RT-qPCR. Fifty-four microRNAs were found to be significantly deregulated in serum of colon cancer patients compared to healthy donors (P < 0.01). A diagnostic 4-microRNA signature consisting of miR-23a-3p, miR-27a-3p, miR-142-5p and miR-376c-3p was established (AUC = 0.917), distinguishing colon cancer patients from healthy donors with sensitivity of 89% and specificity of 81% (AUC = 0.922). This panel of microRNAs exhibited high diagnostic performance also when analyzed separately in colon cancer patients in early stages of the disease (T1-4N0M0; AUC = 0.877). Further, a prognostic panel based on the expression of miR-23a-3p and miR-376c-3p independent of TNM stage was established (HR 2.30; 95% CI 1.44-3.66; P < 0.0004). In summary, highly sensitive signatures of circulating microRNAs enabling non-invasive early detection and prognosis prediction of colon cancer were identified.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NT13549, research and development project

Name: Vytvoření diagnostické sady cirkulujících mikroRNA pro neinvazivní časnou diagnostiku a sledování pacientů s kolorektálním karcinomem

NV16-31765A, research and development project

Name: Využití tkáňových/cirkulujících mikroRNA pro predikci léčebné odpovědi a zpřesnění restagingu karcinomu rekta po neoadjuvantní léčbě

90004, large research infrastructures

Name: BBMRI-CZ

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Citovat

VYCHYTILOVÁ, Petra, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka KOSAŘOVÁ, Kateřina SLABÁ, Pavel FABIAN, Tomáš GROLICH, Vladimír PROCHÁZKA, Zdeněk KALA, Marek SVOBODA, Igor KISS, Rostislav VYZULA and Ondřej SLABÝ. Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer. Carcinogenesis. Oxford: Oxford University Press, 2016, vol. 37, No 10, p. 941-950. ISSN 0143-3334. Available from: https://dx.doi.org/10.1093/carcin/bgw078.

@article{1351259,
   author = {Vychytilová, Petra and Radová, Lenka and Šachlová, Milana and Kosařová, Zdeňka and Slabá, Kateřina and Fabian, Pavel and Grolich, Tomáš and Procházka, Vladimír and Kala, Zdeněk and Svoboda, Marek and Kiss, Igor and Vyzula, Rostislav and Slabý, Ondřej},
   article_location = {Oxford},
   article_number = {10},
   doi = {http://dx.doi.org/10.1093/carcin/bgw078},
   keywords = {microRNA; colorectal cancer; blood serum; diagnostic biomarker; prognostic biomarker},
   language = {eng},
   issn = {0143-3334},
   journal = {Carcinogenesis},
   title = {Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer},
   url = {https://academic.oup.com/carcin/article-abstract/37/10/941/2196570/Serum-based-microRNA-signatures-in-early-diagnosis?redirectedFrom=fulltext},
   volume = {37},
   year = {2016}
}

TY  - JOUR
ID  - 1351259
AU  - Vychytilová, Petra - Radová, Lenka - Šachlová, Milana - Kosařová, Zdeňka - Slabá, Kateřina - Fabian, Pavel - Grolich, Tomáš - Procházka, Vladimír - Kala, Zdeněk - Svoboda, Marek - Kiss, Igor - Vyzula, Rostislav - Slabý, Ondřej
PY  - 2016
TI  - Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer
JF  - Carcinogenesis
VL  - 37
IS  - 10
SP  - 941-950
EP  - 941-950
PB  - Oxford University Press
SN  - 01433334
KW  - microRNA
KW  - colorectal cancer
KW  - blood serum
KW  - diagnostic biomarker
KW  - prognostic biomarker
UR  - https://academic.oup.com/carcin/article-abstract/37/10/941/2196570/Serum-based-microRNA-signatures-in-early-diagnosis?redirectedFrom=fulltext
L2  - https://academic.oup.com/carcin/article-abstract/37/10/941/2196570/Serum-based-microRNA-signatures-in-early-diagnosis?redirectedFrom=fulltext
N2  - Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of the present study was to identify serum microRNAs enabling early diagnosis and prognosis prediction of colon cancer. In total, serum samples from 427 colon cancer patients and 276 healthy donors were included in 3-phase biomarker study. Large-scale microRNA expression profiling was performed using Illumina small RNA sequencing. Diagnostic and prognostic potential of identified microRNAs was validated on independent training and validation sets of samples using RT-qPCR. Fifty-four microRNAs were found to be significantly deregulated in serum of colon cancer patients compared to healthy donors (P < 0.01). A diagnostic 4-microRNA signature consisting of miR-23a-3p, miR-27a-3p, miR-142-5p and miR-376c-3p was established (AUC = 0.917), distinguishing colon cancer patients from healthy donors with sensitivity of 89% and specificity of 81% (AUC = 0.922). This panel of microRNAs exhibited high diagnostic performance also when analyzed separately in colon cancer patients in early stages of the disease (T1-4N0M0; AUC = 0.877). Further, a prognostic panel based on the expression of miR-23a-3p and miR-376c-3p independent of TNM stage was established (HR 2.30; 95% CI 1.44-3.66; P < 0.0004). In summary, highly sensitive signatures of circulating microRNAs enabling non-invasive early detection and prognosis prediction of colon cancer were identified.
ER  -

VYCHYTILOVÁ, Petra, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka KOSAŘOVÁ, Kateřina SLABÁ, Pavel FABIAN, Tomáš GROLICH, Vladimír PROCHÁZKA, Zdeněk KALA, Marek SVOBODA, Igor KISS, Rostislav VYZULA and Ondřej SLABÝ. Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer. \textit{Carcinogenesis}. Oxford: Oxford University Press, 2016, vol.~37, No~10, p.~941-950. ISSN~0143-3334. Available from: https://dx.doi.org/10.1093/carcin/bgw078.

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