Detailed Information on Publication Record
2016
Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer
VYCHYTILOVÁ, Petra, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka KOSAŘOVÁ, Kateřina SLABÁ et. al.Basic information
Original name
Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer
Authors
VYCHYTILOVÁ, Petra (203 Czech Republic, belonging to the institution), Lenka RADOVÁ (203 Czech Republic, belonging to the institution), Milana ŠACHLOVÁ (203 Czech Republic, belonging to the institution), Zdeňka KOSAŘOVÁ (203 Czech Republic, belonging to the institution), Kateřina SLABÁ (203 Czech Republic, belonging to the institution), Pavel FABIAN (203 Czech Republic, belonging to the institution), Tomáš GROLICH (203 Czech Republic, belonging to the institution), Vladimír PROCHÁZKA (203 Czech Republic, belonging to the institution), Zdeněk KALA (203 Czech Republic, belonging to the institution), Marek SVOBODA (203 Czech Republic, belonging to the institution), Igor KISS (203 Czech Republic, belonging to the institution), Rostislav VYZULA (203 Czech Republic, belonging to the institution) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Carcinogenesis, Oxford, Oxford University Press, 2016, 0143-3334
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.105
RIV identification code
RIV/00216224:14740/16:00088870
Organization unit
Central European Institute of Technology
UT WoS
000386037100003
Keywords (in Czech)
mikroRNA; kolorektální karcinom; krevní sérum; diagnostický biomarker; prognostický biomarker
Keywords in English
microRNA; colorectal cancer; blood serum; diagnostic biomarker; prognostic biomarker
Tags
Tags
International impact, Reviewed
Změněno: 26/4/2021 13:39, Mgr. Tereza Miškechová
Abstract
V originále
Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of the present study was to identify serum microRNAs enabling early diagnosis and prognosis prediction of colon cancer. In total, serum samples from 427 colon cancer patients and 276 healthy donors were included in 3-phase biomarker study. Large-scale microRNA expression profiling was performed using Illumina small RNA sequencing. Diagnostic and prognostic potential of identified microRNAs was validated on independent training and validation sets of samples using RT-qPCR. Fifty-four microRNAs were found to be significantly deregulated in serum of colon cancer patients compared to healthy donors (P < 0.01). A diagnostic 4-microRNA signature consisting of miR-23a-3p, miR-27a-3p, miR-142-5p and miR-376c-3p was established (AUC = 0.917), distinguishing colon cancer patients from healthy donors with sensitivity of 89% and specificity of 81% (AUC = 0.922). This panel of microRNAs exhibited high diagnostic performance also when analyzed separately in colon cancer patients in early stages of the disease (T1-4N0M0; AUC = 0.877). Further, a prognostic panel based on the expression of miR-23a-3p and miR-376c-3p independent of TNM stage was established (HR 2.30; 95% CI 1.44-3.66; P < 0.0004). In summary, highly sensitive signatures of circulating microRNAs enabling non-invasive early detection and prognosis prediction of colon cancer were identified.
Links
LQ1601, research and development project |
| ||
NT13549, research and development project |
| ||
NV16-31765A, research and development project |
| ||
90004, large research infrastructures |
|