Rapid Immunosensing of Salmonella Typhimurium Using
Electrochemical Impedance Spectroscopy: the Effect of Sample
Treatment

J 2016

Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA, David KOVÁŘ, Karel LACINA et. al.

Basic information

Original name

Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment

Authors

FARKA, Zdeněk (203 Czech Republic, belonging to the institution), Tomáš JUŘÍK (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution), Karel LACINA (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Electroanalysis, Weinheim, Wiley, 2016, 1040-0397

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.851

RIV identification code

RIV/00216224:14740/16:00090535

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/elan.201600093

UT WoS

000382579300014

Keywords in English

Atomic force microscopy; Biosensor; Immunosensor; Label-free detection; Salmonella Typhimurium

Abstract

V originále

A label-free immunosensor for rapid detection of Salmonella Typhimurium based on electrochemical impedance spectroscopy was developed. Specific antibody was immobilized to a screen-printed electrode via cysteamine monolayer activated with glutaraldehyde and the impedance was measured between two gold electrodes. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. Wide linear response was obtained in the range between 10^3 CFU·mL-1 and 10^8 CFU·mL-1. The successful detection of Salmonella in spiked milk demonstrates the suitability of sensor for the analysis of real samples.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA, David KOVÁŘ, Karel LACINA and Petr SKLÁDAL. Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment. Electroanalysis. Weinheim: Wiley, 2016, vol. 28, No 8, p. 1803-1809. ISSN 1040-0397. Available from: https://dx.doi.org/10.1002/elan.201600093.

@article{1351272,
   author = {Farka, Zdeněk and Juřík, Tomáš and Pastucha, Matěj and Kovář, David and Lacina, Karel and Skládal, Petr},
   article_location = {Weinheim},
   article_number = {8},
   doi = {http://dx.doi.org/10.1002/elan.201600093},
   keywords = {Atomic force microscopy; Biosensor; Immunosensor; Label-free detection; Salmonella Typhimurium},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full},
   volume = {28},
   year = {2016}
}

TY  - JOUR
ID  - 1351272
AU  - Farka, Zdeněk - Juřík, Tomáš - Pastucha, Matěj - Kovář, David - Lacina, Karel - Skládal, Petr
PY  - 2016
TI  - Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment
JF  - Electroanalysis
VL  - 28
IS  - 8
SP  - 1803-1809
EP  - 1803-1809
PB  - Wiley
SN  - 10400397
KW  - Atomic force microscopy
KW  - Biosensor
KW  - Immunosensor
KW  - Label-free detection
KW  - Salmonella Typhimurium
UR  - http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full
L2  - http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full
N2  - A label-free immunosensor for rapid detection of Salmonella Typhimurium based on electrochemical impedance spectroscopy was developed. Specific antibody was immobilized to a screen-printed electrode via cysteamine monolayer activated with glutaraldehyde and the impedance was measured between two gold electrodes. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. Wide linear response was obtained in the range between 10^3 CFU·mL-1 and 10^8 CFU·mL-1. The successful detection of Salmonella in spiked milk demonstrates the suitability of sensor for the analysis of real samples.
ER  -

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA, David KOVÁŘ, Karel LACINA and Petr SKLÁDAL. Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment. \textit{Electroanalysis}. Weinheim: Wiley, 2016, vol.~28, No~8, p.~1803-1809. ISSN~1040-0397. Available from: https://dx.doi.org/10.1002/elan.201600093.

Detailed Information on Publication Record