Quantitative proteomics of the tobacco pollen tube secretome
identifies novel pollen tube guidance proteins important for
fertilization

J 2016

Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization

SAID, Hafidh, David POTĚŠIL, Jan FÍLA, Věra ČAPKOVÁ, Zbyněk ZDRÁHAL et. al.

Basic information

Original name

Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization

Authors

SAID, Hafidh (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), Jan FÍLA (203 Czech Republic), Věra ČAPKOVÁ (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution) and David HONYS (203 Czech Republic)

Edition

Genome Biology, London, BIOMED CENTRAL LTD, 2016, 1465-6906

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 11.313 in 2015

RIV identification code

RIV/00216224:14740/16:00088078

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1186/s13059-016-0928-x

UT WoS

000375962000001

Keywords in English

Protein secretion; Pollen tube guidance; Cell-cell signaling; Double fertilization

Abstract

V originále

We performed genome-wide quantitative liquid chromatography-tandem mass spectrometry analysis of a pistil-stimulated pollen tube secretome and identified 801 pollen tube-secreted proteins. Interestingly, in silico analysis reveals that the pollen tube secretome is dominated by proteins that are secreted unconventionally, representing 57 % of the total secretome. In support, we show that an unconventionally secreted protein, translationally controlled tumor protein, is secreted to the apoplast. Remarkably, we discovered that this protein could be secreted by infiltrating through the initial phases of the conventional secretory pathway and could reach the apoplast via exosomes, as demonstrated by co-localization with Oleisin1 exosome marker. We demonstrate that translationally controlled tumor protein-knockdown Arabidopsis thaliana plants produce pollen tubes that navigate poorly to the target ovule and that the mutant allele is poorly transmitted through the male. Further, we show that regulators of the endoplasmic reticulum-trans-Golgi network protein secretory pathway control secretion of Nicotiana tabacum Pollen tube-secreted cysteine-rich protein 2 and Lorelei-like GPI-anchor protein 3 and that a regulator of endoplasmic reticulum-trans-Golgi protein translocation is essential for pollen tube growth, pollen tube guidance and ovule-targeting competence.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

ED3.2.00/08.0144, research and development project

Name: CERIT Scientific Cloud

GA15-16050S, research and development project

Name: RNA vazebné proteiny účastnící se translační represe skladovaných transkriptů během vývoje samčího gametofytu

Investor: Czech Science Foundation

GA15-22720S, research and development project

Name: Signalizace mezi pylem a pestíkem předcházející oplození; úloha sekretovaných signálů působících na kratší a delší vzdálenost

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SAID, Hafidh, David POTĚŠIL, Jan FÍLA, Věra ČAPKOVÁ, Zbyněk ZDRÁHAL and David HONYS. Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization. Genome Biology. London: BIOMED CENTRAL LTD, 2016, vol. 17, May, p. nestránkováno, 29 pp. ISSN 1465-6906. Available from: https://dx.doi.org/10.1186/s13059-016-0928-x.

@article{1351698,
   author = {Said, Hafidh and Potěšil, David and Fíla, Jan and Čapková, Věra and Zdráhal, Zbyněk and Honys, David},
   article_location = {London},
   article_number = {May},
   doi = {http://dx.doi.org/10.1186/s13059-016-0928-x},
   keywords = {Protein secretion; Pollen tube guidance; Cell-cell signaling; Double fertilization},
   language = {eng},
   issn = {1465-6906},
   journal = {Genome Biology},
   title = {Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization},
   url = {http://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-0928-x},
   volume = {17},
   year = {2016}
}

TY  - JOUR
ID  - 1351698
AU  - Said, Hafidh - Potěšil, David - Fíla, Jan - Čapková, Věra - Zdráhal, Zbyněk - Honys, David
PY  - 2016
TI  - Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization
JF  - Genome Biology
VL  - 17
IS  - May
SP  - nestránkováno
EP  - nestránkováno
PB  - BIOMED CENTRAL LTD
SN  - 14656906
KW  - Protein secretion
KW  - Pollen tube guidance
KW  - Cell-cell signaling
KW  - Double fertilization
UR  - http://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-0928-x
L2  - http://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-0928-x
N2  - We performed genome-wide quantitative liquid chromatography-tandem mass spectrometry analysis of a pistil-stimulated pollen tube secretome and identified 801 pollen tube-secreted proteins. Interestingly, in silico analysis reveals that the pollen tube secretome is dominated by proteins that are secreted unconventionally, representing 57 % of the total secretome. In support, we show that an unconventionally secreted protein, translationally controlled tumor protein, is secreted to the apoplast. Remarkably, we discovered that this protein could be secreted by infiltrating through the initial phases of the conventional secretory pathway and could reach the apoplast via exosomes, as demonstrated by co-localization with Oleisin1 exosome marker. We demonstrate that translationally controlled tumor protein-knockdown Arabidopsis thaliana plants produce pollen tubes that navigate poorly to the target ovule and that the mutant allele is poorly transmitted through the male. Further, we show that regulators of the endoplasmic reticulum-trans-Golgi network protein secretory pathway control secretion of Nicotiana tabacum Pollen tube-secreted cysteine-rich protein 2 and Lorelei-like GPI-anchor protein 3 and that a regulator of endoplasmic reticulum-trans-Golgi protein translocation is essential for pollen tube growth, pollen tube guidance and ovule-targeting competence.
ER  -

SAID, Hafidh, David POTĚŠIL, Jan FÍLA, Věra ČAPKOVÁ, Zbyněk ZDRÁHAL and David HONYS. Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization. \textit{Genome Biology}. London: BIOMED CENTRAL LTD, 2016, vol.~17, May, p.~nestránkováno, 29 pp. ISSN~1465-6906. Available from: https://dx.doi.org/10.1186/s13059-016-0928-x.

Detailed Information on Publication Record