Automatic Detection of Laser-Induced Structures in Live Cell
Fluorescent Microscopy Images Using Snakes with Geometric
Constraints

D 2016

Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints

KONDRAT'EV, Alexandr and Dmitry SOROKIN

Basic information

Original name

Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints

Authors

KONDRAT'EV, Alexandr (643 Russian Federation) and Dmitry SOROKIN (643 Russian Federation, guarantor, belonging to the institution)

Edition

Not specified, IEEE 23rd International Conference on Pattern Recognition (ICPR), p. 326-331, 6 pp. 2016

Publisher

IEEE

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

RIV identification code

RIV/00216224:14330/16:00088097

Organization unit

Faculty of Informatics

ISBN

978-1-5090-4846-5

DOI

http://dx.doi.org/10.1109/ICPR.2016.7899655

Keywords in English

Biological image and signal analysis; Biologically motivated vision; Segmentation features and descriptors

Abstract

V originále

The existence of reliable evaluation datasets for cell image registration algorithms is crucial for quantitative comparison of registration approaches. A new technique for creating real live cell image sequences for this purpose was introduced recently. These datasets contain stable structures bleached by argon laser in the cell nucleus. In this work, we propose an approach for automatic detection of laser-induced linear structures in live cell fluorescent microscopy images. Compared to a previous linear laser-induced structure detection approach, our method employs an active contours model with a Hessian-based image energy term for linear structures enhancement and geometric energy term controlling the geometric relations between the structures. It uses position adaptive tension parameter values to adjust the snakes behavior in problematic regions (end points and intersection points) and a temporal consistent scheme where the results from the previous frame are used as an initial approximation for the current frame. Our approach was successfully applied to real live cell microscopy image sequences and an experimental comparison with an existing laser-induced structures detection method based on minimal paths has been performed.

Links

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

KONDRAT'EV, Alexandr and Dmitry SOROKIN. Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints. Online. In IEEE 23rd International Conference on Pattern Recognition (ICPR). Not specified: IEEE, 2016, p. 326-331. ISBN 978-1-5090-4846-5. Available from: https://dx.doi.org/10.1109/ICPR.2016.7899655.

@inproceedings{1352254,
   author = {Kondrat'ev, Alexandr and Sorokin, Dmitry},
   address = {Not specified},
   booktitle = {IEEE 23rd International Conference on Pattern Recognition (ICPR)},
   doi = {http://dx.doi.org/10.1109/ICPR.2016.7899655},
   keywords = {Biological image and signal analysis; Biologically motivated vision; Segmentation features and descriptors},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Not specified},
   isbn = {978-1-5090-4846-5},
   pages = {326-331},
   publisher = {IEEE},
   title = {Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints},
   year = {2016}
}

TY  - JOUR
ID  - 1352254
AU  - Kondrat'ev, Alexandr - Sorokin, Dmitry
PY  - 2016
TI  - Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints
PB  - IEEE
CY  - Not specified
SN  - 9781509048465
KW  - Biological image and signal analysis
KW  - Biologically motivated vision
KW  - Segmentation features and descriptors
N2  - The existence of reliable evaluation datasets for cell image registration algorithms is crucial for quantitative comparison of registration approaches. A new technique for creating real live cell image sequences for this purpose was introduced recently. These datasets contain stable structures bleached by argon laser in the cell nucleus. In this work, we propose an approach for automatic detection of laser-induced linear structures in live cell fluorescent microscopy images. Compared to a previous linear laser-induced structure detection approach, our method employs an active contours model with a Hessian-based image energy term for linear structures enhancement and geometric energy term controlling the geometric relations between the structures. It uses position adaptive tension parameter values to adjust the snakes behavior in problematic regions (end points and intersection points) and a temporal consistent scheme where the results from the previous frame are used as an initial approximation for the current frame. Our approach was successfully applied to real live cell microscopy image sequences and an experimental comparison with an existing laser-induced structures detection method based on minimal paths has been performed.
ER  -

KONDRAT'EV, Alexandr and Dmitry SOROKIN. Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints. Online. In \textit{IEEE 23rd International Conference on Pattern Recognition (ICPR)}. Not specified: IEEE, 2016, p.~326-331. ISBN~978-1-5090-4846-5. Available from: https://dx.doi.org/10.1109/ICPR.2016.7899655.

Detailed Information on Publication Record