KONDRAT'EV, Alexandr and Dmitry SOROKIN. Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints. Online. In IEEE 23rd International Conference on Pattern Recognition (ICPR). Not specified: IEEE, 2016, p. 326-331. ISBN 978-1-5090-4846-5. Available from: https://dx.doi.org/10.1109/ICPR.2016.7899655.
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Basic information
Original name Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints
Authors KONDRAT'EV, Alexandr (643 Russian Federation) and Dmitry SOROKIN (643 Russian Federation, guarantor, belonging to the institution).
Edition Not specified, IEEE 23rd International Conference on Pattern Recognition (ICPR), p. 326-331, 6 pp. 2016.
Publisher IEEE
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
RIV identification code RIV/00216224:14330/16:00088097
Organization unit Faculty of Informatics
ISBN 978-1-5090-4846-5
Doi http://dx.doi.org/10.1109/ICPR.2016.7899655
Keywords in English Biological image and signal analysis; Biologically motivated vision; Segmentation features and descriptors
Tags CBIA, cbia-web, core_B, firank_A
Changed by Changed by: RNDr. Pavel Šmerk, Ph.D., učo 3880. Changed: 28/8/2024 17:23.
Abstract
The existence of reliable evaluation datasets for cell image registration algorithms is crucial for quantitative comparison of registration approaches. A new technique for creating real live cell image sequences for this purpose was introduced recently. These datasets contain stable structures bleached by argon laser in the cell nucleus. In this work, we propose an approach for automatic detection of laser-induced linear structures in live cell fluorescent microscopy images. Compared to a previous linear laser-induced structure detection approach, our method employs an active contours model with a Hessian-based image energy term for linear structures enhancement and geometric energy term controlling the geometric relations between the structures. It uses position adaptive tension parameter values to adjust the snakes behavior in problematic regions (end points and intersection points) and a temporal consistent scheme where the results from the previous frame are used as an initial approximation for the current frame. Our approach was successfully applied to real live cell microscopy image sequences and an experimental comparison with an existing laser-induced structures detection method based on minimal paths has been performed.
Links
GBP302/12/G157, research and development projectName: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation
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