Application of advanced light microscopic techniques to gain
deeper insights into cheese matrix physico-chemistry

J 2015

Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

BURDIKOVA, Z, Z SVINDRYCH, C HICKEY, MG WILKINSON, MAE AUTY et. al.

Basic information

Original name

Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Authors

BURDIKOVA, Z, Z SVINDRYCH, C HICKEY, MG WILKINSON, MAE AUTY, Ota SAMEK, Silvie BERNATOVÁ, Vladislav KRZYŽÁNEK, A PERIASAMY and JJ SHEEHAN

Edition

DAIRY SCIENCE & TECHNOLOGY, PARIS, SPRINGER FRANCE, 2015, 1958-5586

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.435

DOI

http://dx.doi.org/10.1007/s13594-015-0253-2

UT WoS

000361480900009

Keywords in English

Confocal microscopy; Cheese matrix; Fluorescence lifetime; Second harmonic generation; Two-photon excitation; Confocal Raman microscopy

Změněno: 29/8/2016 10:19, Mgr. Silvie Bernatová, Ph.D.

Abstract

V originále

Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.

Citovat

BURDIKOVA, Z, Z SVINDRYCH, C HICKEY, MG WILKINSON, MAE AUTY, Ota SAMEK, Silvie BERNATOVÁ, Vladislav KRZYŽÁNEK, A PERIASAMY and JJ SHEEHAN. Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry. DAIRY SCIENCE & TECHNOLOGY. PARIS: SPRINGER FRANCE, 2015, vol. 95, No 5, p. 687-700. ISSN 1958-5586. Available from: https://dx.doi.org/10.1007/s13594-015-0253-2.

@article{1352645,
   author = {Burdikova, Z and Svindrych, Z and Hickey, C and Wilkinson, MG and Auty, MAE and Samek, Ota and Bernatová, Silvie and Krzyžánek, Vladislav and Periasamy, A and Sheehan, JJ},
   article_location = {PARIS},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s13594-015-0253-2},
   keywords = {Confocal microscopy; Cheese matrix; Fluorescence lifetime; Second harmonic generation; Two-photon excitation; Confocal Raman microscopy},
   language = {eng},
   issn = {1958-5586},
   journal = {DAIRY SCIENCE & TECHNOLOGY},
   title = {Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry},
   url = {http://dx.doi.org/10.1007/s13594-015-0253-2},
   volume = {95},
   year = {2015}
}

TY  - JOUR
ID  - 1352645
AU  - Burdikova, Z - Svindrych, Z - Hickey, C - Wilkinson, MG - Auty, MAE - Samek, Ota - Bernatová, Silvie - Krzyžánek, Vladislav - Periasamy, A - Sheehan, JJ
PY  - 2015
TI  - Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry
JF  - DAIRY SCIENCE & TECHNOLOGY
VL  - 95
IS  - 5
SP  - 687-700
EP  - 687-700
PB  - SPRINGER FRANCE
SN  - 19585586
KW  - Confocal microscopy
KW  - Cheese matrix
KW  - Fluorescence lifetime
KW  - Second harmonic generation
KW  - Two-photon excitation
KW  - Confocal Raman microscopy
UR  - http://dx.doi.org/10.1007/s13594-015-0253-2
L2  - http://dx.doi.org/10.1007/s13594-015-0253-2
N2  - Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.
ER  -

BURDIKOVA, Z, Z SVINDRYCH, C HICKEY, MG WILKINSON, MAE AUTY, Ota SAMEK, Silvie BERNATOVÁ, Vladislav KRZYŽÁNEK, A PERIASAMY and JJ SHEEHAN. Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry. \textit{DAIRY SCIENCE \&{}amp; TECHNOLOGY}. PARIS: SPRINGER FRANCE, 2015, vol.~95, No~5, p.~687-700. ISSN~1958-5586. Available from: https://dx.doi.org/10.1007/s13594-015-0253-2.

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