FARKA, Zdeněk, Tomáš JUŘÍK, Pavel PODEŠVA, David KOVÁŘ, František FORET and Petr SKLÁDAL. Gelatin-templated Nanostructured Gold for Electrochemical Detection of Glucose in Serum. In 67th Annual Meeting of the International Society of Electrochemistry. 2016.
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Basic information
Original name Gelatin-templated Nanostructured Gold for Electrochemical Detection of Glucose in Serum
Authors FARKA, Zdeněk, Tomáš JUŘÍK, Pavel PODEŠVA, David KOVÁŘ, František FORET and Petr SKLÁDAL.
Edition 67th Annual Meeting of the International Society of Electrochemistry, 2016.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10405 Electrochemistry
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Organization unit Central European Institute of Technology
Keywords in English Gold nanostructures; Gelatin template; Glucose electrooxidation; Non-enzymatic sensor; Blood analysis
Tags International impact
Changed by Changed by: doc. Mgr. Zdeněk Farka, Ph.D., učo 357740. Changed: 30/8/2016 15:48.
Abstract
Nanostructured electrodes based on gelatin-templated gold were developed for sensitive non-enzymatic detection of glucose. The structures of specific saw tooth-like morphology were formed by electrodeposition of gold through the cavities of crosslinked gelatin. The growth of nanostructures was investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electroactive surface area (ESA) and electrochemical behavior of the prepared electrodes were determined by the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The roughness factor (expressed as ESA divided by geometrical area) of the gelatin-templated surface was estimated to be 16.7, which represent 15.9 fold enlargement compared to the flat gold electrode. The nanoscopic surface exhibited strong electrocatalytic effect towards glucose oxidation. The detection of glucose was done by direct electrochemical oxidation during CV and amperometry. In case of CV, the sensitivity of 1165 μA·mM-1·cm-2, wide linear range from 32 μM to 10 mM and limit of detection (LOD) of 90 μM were achieved. The amperometry allowed to further improve the LOD to 1.5 μM with very low applied potential of 0 V vs Ag. The detection of glucose in deproteinized human serum samples was possible with negligible interference of oxidizable blood components. The results were verified by a commercial glucometer and standard kit for photometric detection of glucose. Strong correlation between the methods was confirmed; the determination of glucose in serum was performed with a relative standard deviation of 4 %. The developed sensor exhibits a great potential for rapid and sensitive non-enzymatic glucose detection in clinical applications.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
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