Genomewide profiling of copy-number alteration in monoclonal
gammopathy of undetermined significance

J 2016

Genomewide profiling of copy-number alteration in monoclonal gammopathy of undetermined significance

MIKULÁŠOVÁ, Aneta, Jan SMETANA, Markéta WAYHELOVÁ, Helena JANYŠKOVÁ, Viera SANDECKÁ et. al.

Basic information

Original name

Genomewide profiling of copy-number alteration in monoclonal gammopathy of undetermined significance

Authors

MIKULÁŠOVÁ, Aneta (203 Czech Republic, guarantor, belonging to the institution), Jan SMETANA (203 Czech Republic, belonging to the institution), Markéta WAYHELOVÁ (203 Czech Republic, belonging to the institution), Helena JANYŠKOVÁ (203 Czech Republic, belonging to the institution), Viera SANDECKÁ (703 Slovakia), Zuzana KUFOVÁ (203 Czech Republic), Martina ALMÁŠI (203 Czech Republic), Jiří JARKOVSKÝ (203 Czech Republic, belonging to the institution), Evzen GREGORA (203 Czech Republic), Petr KESSLER (203 Czech Republic), Marek WROBEL (203 Czech Republic), Brian WALKER (826 United Kingdom of Great Britain and Northern Ireland), Christopher WARDELL (826 United Kingdom of Great Britain and Northern Ireland), Gareth MORGAN (826 United Kingdom of Great Britain and Northern Ireland), Roman HÁJEK (203 Czech Republic) and Petr KUGLÍK (203 Czech Republic, belonging to the institution)

Edition

European Journal of Haematology, Wiley-Blackwell Munksgaard, 2016, 0902-4441

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.653

RIV identification code

RIV/00216224:14310/16:00088878

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/ejh.12774

UT WoS

000388632400010

Keywords in English

DNA copy-number changes; DNA microarrays; monoclonal gammopathies; prognosis

Abstract

V originále

Monoclonal gammopathy of undetermined significance (MGUS) is a benign condition with an approximate 1% annual risk of symptomatic plasma cell disorder development, mostly to multiple myeloma (MM). We performed genome-wide screening of copy-number alterations (CNAs) in 90 MGUS and 33 MM patients using high-density DNA microarrays. We identified CNAs in a smaller proportion of MGUS (65.6%) than in MM (100.0%, p=1.31×10-5 ) and showed median number of CNAs is lower in MGUS (3, range 0-22) than in MM (13, range 4-38, p=1.82×10-10 ). In the MGUS cohort, the most frequent losses were located at 1p (5.6%), 6q (6.7%), 13q (30.0%), 14q (14.4%), 16q (8.9%), 21q (5.6%) and gains at 1q (23.3%), 2p (6.7%), 6p (13.3%) and Xq (7.8%). Hyperdiploidy was detected in 38.9% of MGUS cases and the most frequent whole chromosome gains were 3 (25.6%), 5 (23.3%), 9 (37.8%), 15 (23.3%) and 19 (32.2%). We also identified CNAs such as 1p, 6q, 8p, 12p, 13q, 16q losses, 1q gain and hypodiploidy, which are potentially associated with an adverse prognosis in MGUS. In summary, we showed that MGUS is similar to MM in that it is a genetically heterogeneous disorder, but overall cytogenetic instability is lower than in MM, which confirms that genetic abnormalities play important role in monoclonal gammopathies.

Links

NT13492, research and development project

Name: Úloha genetických abnormalit ve vývoji a progresi prekancerózy monoklonální gamapatie nejasného významu

Citovat

MIKULÁŠOVÁ, Aneta, Jan SMETANA, Markéta WAYHELOVÁ, Helena JANYŠKOVÁ, Viera SANDECKÁ, Zuzana KUFOVÁ, Martina ALMÁŠI, Jiří JARKOVSKÝ, Evzen GREGORA, Petr KESSLER, Marek WROBEL, Brian WALKER, Christopher WARDELL, Gareth MORGAN, Roman HÁJEK and Petr KUGLÍK. Genomewide profiling of copy-number alteration in monoclonal gammopathy of undetermined significance. European Journal of Haematology. Wiley-Blackwell Munksgaard, 2016, vol. 97, No 6, p. 568-575. ISSN 0902-4441. Available from: https://dx.doi.org/10.1111/ejh.12774.

@article{1352757,
   author = {Mikulášová, Aneta and Smetana, Jan and Wayhelová, Markéta and Janyšková, Helena and Sandecká, Viera and Kufová, Zuzana and Almáši, Martina and Jarkovský, Jiří and Gregora, Evzen and Kessler, Petr and Wrobel, Marek and Walker, Brian and Wardell, Christopher and Morgan, Gareth and Hájek, Roman and Kuglík, Petr},
   article_number = {6},
   doi = {http://dx.doi.org/10.1111/ejh.12774},
   keywords = {DNA copy-number changes; DNA microarrays; monoclonal gammopathies; prognosis},
   language = {eng},
   issn = {0902-4441},
   journal = {European Journal of Haematology},
   title = {Genomewide profiling of copy-number alteration in monoclonal gammopathy of undetermined significance},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/ejh.12774/full},
   volume = {97},
   year = {2016}
}

TY  - JOUR
ID  - 1352757
AU  - Mikulášová, Aneta - Smetana, Jan - Wayhelová, Markéta - Janyšková, Helena - Sandecká, Viera - Kufová, Zuzana - Almáši, Martina - Jarkovský, Jiří - Gregora, Evzen - Kessler, Petr - Wrobel, Marek - Walker, Brian - Wardell, Christopher - Morgan, Gareth - Hájek, Roman - Kuglík, Petr
PY  - 2016
TI  - Genomewide profiling of copy-number alteration in monoclonal gammopathy of undetermined significance
JF  - European Journal of Haematology
VL  - 97
IS  - 6
SP  - 568-575
EP  - 568-575
PB  - Wiley-Blackwell Munksgaard
SN  - 09024441
KW  - DNA copy-number changes
KW  - DNA microarrays
KW  - monoclonal gammopathies
KW  - prognosis
UR  - http://onlinelibrary.wiley.com/doi/10.1111/ejh.12774/full
N2  - Monoclonal gammopathy of undetermined significance (MGUS) is a benign condition with an approximate 1% annual risk of symptomatic plasma cell disorder development, mostly to multiple myeloma (MM). We performed genome-wide screening of copy-number alterations (CNAs) in 90 MGUS and 33 MM patients using high-density DNA microarrays. We identified CNAs in a smaller proportion of MGUS (65.6%) than in MM (100.0%, p=1.31×10-5 ) and showed median number of CNAs is lower in MGUS (3, range 0-22) than in MM (13, range 4-38, p=1.82×10-10 ). In the MGUS cohort, the most frequent losses were located at 1p (5.6%), 6q (6.7%), 13q (30.0%), 14q (14.4%), 16q (8.9%), 21q (5.6%) and gains at 1q (23.3%), 2p (6.7%), 6p (13.3%) and Xq (7.8%). Hyperdiploidy was detected in 38.9% of MGUS cases and the most frequent whole chromosome gains were 3 (25.6%), 5 (23.3%), 9 (37.8%), 15 (23.3%) and 19 (32.2%). We also identified CNAs such as 1p, 6q, 8p, 12p, 13q, 16q losses, 1q gain and hypodiploidy, which are potentially associated with an adverse prognosis in MGUS. In summary, we showed that MGUS is similar to MM in that it is a genetically heterogeneous disorder, but overall cytogenetic instability is lower than in MM, which confirms that genetic abnormalities play important role in monoclonal gammopathies.
ER  -

MIKULÁŠOVÁ, Aneta, Jan SMETANA, Markéta WAYHELOVÁ, Helena JANYŠKOVÁ, Viera SANDECKÁ, Zuzana KUFOVÁ, Martina ALMÁŠI, Jiří JARKOVSKÝ, Evzen GREGORA, Petr KESSLER, Marek WROBEL, Brian WALKER, Christopher WARDELL, Gareth MORGAN, Roman HÁJEK and Petr KUGLÍK. Genomewide profiling of copy-number alteration in monoclonal gammopathy of undetermined significance. \textit{European Journal of Haematology}. Wiley-Blackwell Munksgaard, 2016, vol.~97, No~6, p.~568-575. ISSN~0902-4441. Available from: https://dx.doi.org/10.1111/ejh.12774.

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