RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA and Michal HOCEK. Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions. CHEMICAL SCIENCE. Cambridge: ROYAL SOC CHEMISTRY, 2012, vol. 3, No 9, p. 2797-2806. ISSN 2041-6520. Available from: https://dx.doi.org/10.1039/c2sc20404e. |
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@article{1353378, author = {Riedl, J and Pohl, R and Ernsting, NP and Orság, Petr and Fojta, Miroslav and Hocek, Michal}, article_location = {Cambridge}, article_number = {9}, doi = {http://dx.doi.org/10.1039/c2sc20404e}, language = {eng}, issn = {2041-6520}, journal = {CHEMICAL SCIENCE}, title = {Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions}, volume = {3}, year = {2012} }
TY - JOUR ID - 1353378 AU - Riedl, J - Pohl, R - Ernsting, NP - Orság, Petr - Fojta, Miroslav - Hocek, Michal PY - 2012 TI - Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions JF - CHEMICAL SCIENCE VL - 3 IS - 9 SP - 2797-2806 EP - 2797-2806 PB - ROYAL SOC CHEMISTRY SN - 20416520 N2 - Solvatochromic fluorescent 4-aminophthalimide (API) and 4-(N, N-dimethylamino) phthalimide (DAPI) were attached covalently to 2'-deoxycytidine or -adenosine via a non-conjugated propargyl linker by Sonogashira cross-coupling reactions of N-propargylphthalimides with halogenated nucleosides. The nucleosides were phosphorylated to triphosphates and enzymatically incorporated into oligonucleotides by DNA polymerases. API-labelled DNA was used for the detection of DNA protein interactions with either the sequence-specific p53 protein or a non-specific single strand binding (SSB) protein. Both proteins changed the polarity around the fluorophore and increased (2-3 fold) the intensity of API fluorescence. ER -
RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA and Michal HOCEK. Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions. \textit{CHEMICAL SCIENCE}. Cambridge: ROYAL SOC CHEMISTRY, 2012, vol.~3, No~9, p.~2797-2806. ISSN~2041-6520. Available from: https://dx.doi.org/10.1039/c2sc20404e.
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