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@article{1353472,
author = {Škoda, Jan and Hermanová, Markéta and Loja, Tomáš and Němec, Pavel and Neradil, Jakub and Karásek, Petr and Veselská, Renata},
article_location = {San Francisco},
article_number = {7},
doi = {http://dx.doi.org/10.1371/journal.pone.0159255},
keywords = {pancreatic adenocarcinoma;cancer stem cell markers;expression profilimg},
language = {eng},
issn = {1932-6203},
journal = {PLOS One},
title = {Co-expression of cancer stem cell markers corresponds to a pro-tumorigenic expression profile in pancreatic adenocarcinoma},
url = {http://dx.plos.org/10.1371/journal.pone.0159255},
volume = {11},
year = {2016}
}
TY - JOUR
ID - 1353472
AU - Škoda, Jan - Hermanová, Markéta - Loja, Tomáš - Němec, Pavel - Neradil, Jakub - Karásek, Petr - Veselská, Renata
PY - 2016
TI - Co-expression of cancer stem cell markers corresponds to a pro-tumorigenic expression profile in pancreatic adenocarcinoma
JF - PLOS One
VL - 11
IS - 7
SP - "e0159255"
EP - "e0159255"
PB - PUBLIC LIBRARY SCIENCE
SN - 19326203
KW - pancreatic adenocarcinoma;cancer stem cell markers;expression profilimg
UR - http://dx.plos.org/10.1371/journal.pone.0159255
L2 - http://dx.plos.org/10.1371/journal.pone.0159255
N2 - Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. Its dismal prognosis is often attributed to the presence of cancer stem cells (CSCs) that have been identified in PDAC using various markers. However, the co-expression of all of these markers has not yet been evaluated. Furthermore, studies that compare the expression levels of CSC markers in PDAC tumor samples and in cell lines derived directly from those tumors are lacking. Here, we analyzed the expression of putative CSC markers-CD24, CD44, epithelial cell adhesion molecule (EpCAM), CD133, and nestin-by immunofluorescence, flow cytometry and quantitative PCR in 3 PDAC-derived cell lines and by immunohistochemistry in 3 corresponding tumor samples. We showed high expression of the examined CSC markers among all of the cell lines and tumor samples, with the exception of CD24 and CD44, which were enriched under in vitro conditions compared with tumor tissues. The proportions of cells positive for the remaining markers were comparable to those detected in the corresponding tumors. Co-expression analysis using flow cytometry revealed that CD24+/CD44+/EpCAM+/CD133+ cells represented a significant population of the cells (range, 43 to 72%) among the cell lines. The highest proportion of CD24+/CD44+/EpCAM+/CD133+ cells was detected in the cell line derived from the tumor of a patient with the shortest survival. Using gene expression profiling, we further identified the specific pro-tumorigenic expression profile of this cell line compared with the profiles of the other two cell lines. Together, CD24+/CD44+/EpCAM+/CD133+ cells are present in PDAC cell lines derived from primary tumors, and their increased proportion corresponds with a pro-tumorigenic gene expression profile.
ER -
ŠKODA, Jan, Markéta HERMANOVÁ, Tomáš LOJA, Pavel NĚMEC, Jakub NERADIL, Petr KARÁSEK a Renata VESELSKÁ. Co-expression of cancer stem cell markers corresponds to a pro-tumorigenic expression profile in pancreatic adenocarcinoma. Online. \textit{PLOS One}. San Francisco: PUBLIC LIBRARY SCIENCE, 2016, roč.~11, č.~7, s.~''e0159255'', 18 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0159255. [citováno 2024-04-23]
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