Co-expression of cancer stem cell markers corresponds to a
pro-tumorigenic expression profile in pancreatic adenocarcinoma

J 2016

Co-expression of cancer stem cell markers corresponds to a pro-tumorigenic expression profile in pancreatic adenocarcinoma

ŠKODA, Jan, Markéta HERMANOVÁ, Tomáš LOJA, Pavel NĚMEC, Jakub NERADIL et. al.

Basic information

Original name

Co-expression of cancer stem cell markers corresponds to a pro-tumorigenic expression profile in pancreatic adenocarcinoma

Authors

ŠKODA, Jan (203 Czech Republic, belonging to the institution), Markéta HERMANOVÁ (203 Czech Republic, belonging to the institution), Tomáš LOJA (703 Slovakia, belonging to the institution), Pavel NĚMEC (203 Czech Republic, belonging to the institution), Jakub NERADIL (203 Czech Republic, belonging to the institution), Petr KARÁSEK (203 Czech Republic) and Renata VESELSKÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLOS One, San Francisco, PUBLIC LIBRARY SCIENCE, 2016, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.806

RIV identification code

RIV/00216224:14310/16:00095874

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1371/journal.pone.0159255

UT WoS

000379579500112

Keywords in English

pancreatic adenocarcinoma;cancer stem cell markers;expression profilimg

Abstract

V originále

Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. Its dismal prognosis is often attributed to the presence of cancer stem cells (CSCs) that have been identified in PDAC using various markers. However, the co-expression of all of these markers has not yet been evaluated. Furthermore, studies that compare the expression levels of CSC markers in PDAC tumor samples and in cell lines derived directly from those tumors are lacking. Here, we analyzed the expression of putative CSC markers-CD24, CD44, epithelial cell adhesion molecule (EpCAM), CD133, and nestin-by immunofluorescence, flow cytometry and quantitative PCR in 3 PDAC-derived cell lines and by immunohistochemistry in 3 corresponding tumor samples. We showed high expression of the examined CSC markers among all of the cell lines and tumor samples, with the exception of CD24 and CD44, which were enriched under in vitro conditions compared with tumor tissues. The proportions of cells positive for the remaining markers were comparable to those detected in the corresponding tumors. Co-expression analysis using flow cytometry revealed that CD24+/CD44+/EpCAM+/CD133+ cells represented a significant population of the cells (range, 43 to 72%) among the cell lines. The highest proportion of CD24+/CD44+/EpCAM+/CD133+ cells was detected in the cell line derived from the tumor of a patient with the shortest survival. Using gene expression profiling, we further identified the specific pro-tumorigenic expression profile of this cell line compared with the profiles of the other two cell lines. Together, CD24+/CD44+/EpCAM+/CD133+ cells are present in PDAC cell lines derived from primary tumors, and their increased proportion corresponds with a pro-tumorigenic gene expression profile.

Links

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

Citovat

ŠKODA, Jan, Markéta HERMANOVÁ, Tomáš LOJA, Pavel NĚMEC, Jakub NERADIL, Petr KARÁSEK and Renata VESELSKÁ. Co-expression of cancer stem cell markers corresponds to a pro-tumorigenic expression profile in pancreatic adenocarcinoma. PLOS One. San Francisco: PUBLIC LIBRARY SCIENCE, 2016, vol. 11, No 7, p. "e0159255", 18 pp. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0159255.

@article{1353472,
   author = {Škoda, Jan and Hermanová, Markéta and Loja, Tomáš and Němec, Pavel and Neradil, Jakub and Karásek, Petr and Veselská, Renata},
   article_location = {San Francisco},
   article_number = {7},
   doi = {http://dx.doi.org/10.1371/journal.pone.0159255},
   keywords = {pancreatic adenocarcinoma;cancer stem cell markers;expression profilimg},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS One},
   title = {Co-expression of cancer stem cell markers corresponds to a pro-tumorigenic expression profile in pancreatic adenocarcinoma},
   url = {http://dx.plos.org/10.1371/journal.pone.0159255},
   volume = {11},
   year = {2016}
}

TY  - JOUR
ID  - 1353472
AU  - Škoda, Jan - Hermanová, Markéta - Loja, Tomáš - Němec, Pavel - Neradil, Jakub - Karásek, Petr - Veselská, Renata
PY  - 2016
TI  - Co-expression of cancer stem cell markers corresponds to a pro-tumorigenic expression profile in pancreatic adenocarcinoma
JF  - PLOS One
VL  - 11
IS  - 7
SP  - "e0159255"
EP  - "e0159255"
PB  - PUBLIC LIBRARY SCIENCE
SN  - 19326203
KW  - pancreatic adenocarcinoma;cancer stem cell markers;expression profilimg
UR  - http://dx.plos.org/10.1371/journal.pone.0159255
L2  - http://dx.plos.org/10.1371/journal.pone.0159255
N2  - Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. Its dismal prognosis is often attributed to the presence of cancer stem cells (CSCs) that have been identified in PDAC using various markers. However, the co-expression of all of these markers has not yet been evaluated. Furthermore, studies that compare the expression levels of CSC markers in PDAC tumor samples and in cell lines derived directly from those tumors are lacking. Here, we analyzed the expression of putative CSC markers-CD24, CD44, epithelial cell adhesion molecule (EpCAM), CD133, and nestin-by immunofluorescence, flow cytometry and quantitative PCR in 3 PDAC-derived cell lines and by immunohistochemistry in 3 corresponding tumor samples. We showed high expression of the examined CSC markers among all of the cell lines and tumor samples, with the exception of CD24 and CD44, which were enriched under in vitro conditions compared with tumor tissues. The proportions of cells positive for the remaining markers were comparable to those detected in the corresponding tumors. Co-expression analysis using flow cytometry revealed that CD24+/CD44+/EpCAM+/CD133+ cells represented a significant population of the cells (range, 43 to 72%) among the cell lines. The highest proportion of CD24+/CD44+/EpCAM+/CD133+ cells was detected in the cell line derived from the tumor of a patient with the shortest survival. Using gene expression profiling, we further identified the specific pro-tumorigenic expression profile of this cell line compared with the profiles of the other two cell lines. Together, CD24+/CD44+/EpCAM+/CD133+ cells are present in PDAC cell lines derived from primary tumors, and their increased proportion corresponds with a pro-tumorigenic gene expression profile.
ER  -

ŠKODA, Jan, Markéta HERMANOVÁ, Tomáš LOJA, Pavel NĚMEC, Jakub NERADIL, Petr KARÁSEK and Renata VESELSKÁ. Co-expression of cancer stem cell markers corresponds to a pro-tumorigenic expression profile in pancreatic adenocarcinoma. \textit{PLOS One}. San Francisco: PUBLIC LIBRARY SCIENCE, 2016, vol.~11, No~7, p.~''e0159255'', 18 pp. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0159255.

Detailed Information on Publication Record