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@article{1354102, author = {Fajkus, Petr and Peška, Vratislav and Šeděnka, Zdeňka and Fulnečková, Jana and Dvořáčková, Martina and Gogela, Roman and Sýkorová, Eva and Hapala, Jan and Fajkus, Jiří}, article_location = {Hoboken}, article_number = {3}, doi = {http://dx.doi.org/10.1111/tpj.13115}, keywords = {Allium; unusual telomere; telomerase; TERT; evolution; onion; genome sequencing}, language = {eng}, issn = {0960-7412}, journal = {The Plant Journal}, title = {Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase}, url = {http://onlinelibrary.wiley.com/doi/10.1111/tpj.13115/abstract;jsessionid=DD9460E772BBCE39FC663F17EDC51B3F.f02t03}, volume = {85}, year = {2016} }
TY - JOUR ID - 1354102 AU - Fajkus, Petr - Peška, Vratislav - Šeděnka, Zdeňka - Fulnečková, Jana - Dvořáčková, Martina - Gogela, Roman - Sýkorová, Eva - Hapala, Jan - Fajkus, Jiří PY - 2016 TI - Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase JF - The Plant Journal VL - 85 IS - 3 SP - 337-347 EP - 337-347 PB - Wiley-Blackwell SN - 09607412 KW - Allium KW - unusual telomere KW - telomerase KW - TERT KW - evolution KW - onion KW - genome sequencing UR - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13115/abstract;jsessionid=DD9460E772BBCE39FC663F17EDC51B3F.f02t03 L2 - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13115/abstract;jsessionid=DD9460E772BBCE39FC663F17EDC51B3F.f02t03 N2 - Phylogenetic divergence in Asparagales plants is associated with switches in telomere sequences. The last switch occurred with divergence of the genus Allium (Amaryllidaceae) from the other Allioideae (formerly Alliaceae) genera, resulting in uncharacterized telomeres maintained by an unknown mechanism. To characterize the unknown Allium telomeres, we applied a combination of bioinformatic processing of transcriptomic and genomic data with standard approaches in telomere biology such as BAL31 sensitivity tests, terminal restriction fragment analysis, the telomere repeat amplification protocol (TRAP), and fluorescence insitu hybridization (FISH). Using these methods, we characterize the unusual telomeric sequence (CTCGGTTATGGG)(n) present in Allium species, demonstrate its synthesis by telomerase, and characterize the telomerase reverse transcriptase (TERT) subunit of Allium cepa. Our findings open up the possibility of studying the molecular details of the evolutionary genetic change in Allium telomeres and its possible role in speciation. Experimental studies addressing the implications of this change in terms of the interplay of telomere components may now be designed to shed more light on telomere functions and evolution in general. ER -
FAJKUS, Petr, Vratislav PEŠKA, Zdeňka ŠEDĚNKA, Jana FULNEČKOVÁ, Martina DVOŘÁČKOVÁ, Roman GOGELA, Eva SÝKOROVÁ, Jan HAPALA and Jiří FAJKUS. Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase. \textit{The Plant Journal}. Hoboken: Wiley-Blackwell, 2016, vol.~85, No~3, p.~337-347. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.13115.
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