ROBLOVÁ, Vendula, Miroslava BITTOVÁ, Petr KUBÁŇ and Vlastimil KUBÁŇ. Capillary electrophoresis fingerprinting and spectrophotometric determination of antioxidant potential for classification of Mentha products. Online. Journal of Separation Science. Germany: WILEY-V C H VERLAG GMBH, 2016, vol. 39, No 14, p. 2862-2868. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201600235. [citováno 2024-04-24]
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Basic information
Original name Capillary electrophoresis fingerprinting and spectrophotometric determination of antioxidant potential for classification of Mentha products
Authors ROBLOVÁ, Vendula (203 Czech Republic, belonging to the institution), Miroslava BITTOVÁ (203 Czech Republic, belonging to the institution), Petr KUBÁŇ (203 Czech Republic, belonging to the institution) and Vlastimil KUBÁŇ (203 Czech Republic, guarantor)
Edition Journal of Separation Science, Germany, WILEY-V C H VERLAG GMBH, 2016, 1615-9306.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.557
RIV identification code RIV/00216224:14310/16:00090828
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1002/jssc.201600235
UT WoS 000380728700024
Keywords (in Czech) kapilární elektroforéza, fingerprint, Máta peprná, mátový čaj
Keywords in English Capillary electrophoresis; Fingerprinting; Infusions; Mentha x piperita; Peppermint tea
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 30/3/2017 14:37.
Abstract
In this work aqueous infusions from ten Mentha herbal samples (four different Mentha species and six hybrids of Mentha x piperita) and 20 different peppermint teas were screened by capillary electrophoresis with UV detection. The fingerprint separation was accomplished in a 25 mM borate background electrolyte with 10% methanol at pH 9.3. The total polyphenolic content in the extracts was determined spectrophotometrically at 765 nm by a Folin-Ciocalteu phenol assay. Total antioxidant activity was determined by scavenging of 2,2-diphenyl-1-picrylhydrazyl radical at 515 nm. The peak areas of 12 dominant peaks from CE analysis, present in all samples, and the value of total polyphenolic content and total antioxidant activity obtained by spectrophotometry was combined into a single data matrix and principal component analysis was applied. The obtained principal component analysis model resulted in distinct clusters of Mentha and peppermint tea samples distinguishing the samples according to their potential protective antioxidant effect. Principal component analysis, using a non-targeted approach with no need for compound identification, was found as a new promising tool for the screening of herbal tea products.
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