The motility in selenidiid archigregarines (Selenidiidae), an
early emerging group of Apicomplexa

a 2016

The motility in selenidiid archigregarines (Selenidiidae), an early emerging group of Apicomplexa

KOVÁČIKOVÁ, Magdaléna, Andrei DIAKIN, Gita G. PASKEROVA and Andrea BARDŮNEK VALIGUROVÁ

Basic information

Original name

The motility in selenidiid archigregarines (Selenidiidae), an early emerging group of Apicomplexa

Authors

KOVÁČIKOVÁ, Magdaléna (703 Slovakia, guarantor, belonging to the institution), Andrei DIAKIN (643 Russian Federation, belonging to the institution), Gita G. PASKEROVA (643 Russian Federation) and Andrea BARDŮNEK VALIGUROVÁ (703 Slovakia)

Edition

12th European Multicolloquium of Parasitology, 2016

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Finland

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/16:00088153

Organization unit

Faculty of Science

Keywords in English

Archigregarinidae; Selenidium; motility

Abstract

V originále

Apicomplexans belong to the most monitored group of protists, comprising exclusively parasites of human and animals. Gregarines represent one of the groups with a remarkable diversity a basal lineage restricted to invertebrate hosts. Archigregarines occur only in marine environments and are expected to be the most recent ancestor of gregarines and perhaps apicomplexans as a whole, because of their morphological and life history characteristics. In general, gregarines exhibit various forms of motility, which seem to depend on cytoskeletal structures (subpellicular microtubules, intermediate filaments, actin and myosin). Selenidiid archigregarines exhibit a pendular or rolling movement. Their surface, covered by a typical threemembrane pellicle displays broad folds, beneath each sets of parallel longitudinal subpellicular microtubules are present. In this study experiments using cytoskeletal drugs were performed on living individuals of Selenidium sp., parasitising the intestine of marine polychaete Pygospio elegans (Spionidae). Two probes, cytochalasine D and jasplakinolide, influencing the polymerisation of actin were applied to determine the role of actin filaments in motility. Furthermore, drugs that destroy existing microtubules and inhibit tubulin polymerization, oryzalin and colchicine, were used to confirm the role of subpellicular microtubules. Expected results of this study should verify the fundamental role of cytoskeletal proteins, actin and tubulin, in archigregarine motility and compare their movement mechanism to the substratedependent gliding machinery (= the so called “glideosome” concept) described in apicomplexan zoites. The authors acknowledge the financial support from a project No. GBP505/12/G112 (ECIP) from Czech Science Foundation, and Assoc. prof. T. G. Simdyanov for help with material collection.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

KOVÁČIKOVÁ, Magdaléna, Andrei DIAKIN, Gita G. PASKEROVA and Andrea BARDŮNEK VALIGUROVÁ. The motility in selenidiid archigregarines (Selenidiidae), an early emerging group of Apicomplexa. In 12th European Multicolloquium of Parasitology. 2016.

@proceedings{1354194,
   author = {Kováčiková, Magdaléna and Diakin, Andrei and Paskerova, Gita G. and Bardůnek Valigurová, Andrea},
   booktitle = {12th European Multicolloquium of Parasitology},
   keywords = {Archigregarinidae; Selenidium; motility},
   language = {eng},
   title = {The motility in selenidiid archigregarines (Selenidiidae), an early emerging group of Apicomplexa},
   year = {2016}
}

TY  - CONF
ID  - 1354194
AU  - Kováčiková, Magdaléna - Diakin, Andrei - Paskerova, Gita G. - Bardůnek Valigurová, Andrea
PY  - 2016
TI  - The motility in selenidiid archigregarines (Selenidiidae), an early emerging group of Apicomplexa
KW  - Archigregarinidae
KW  - Selenidium
KW  - motility
N2  - Apicomplexans belong to the most monitored group of protists, comprising exclusively parasites of human and animals. Gregarines represent one of the groups with a remarkable diversity a basal lineage restricted to invertebrate hosts. Archigregarines occur only in marine environments and are expected to be the most recent ancestor of gregarines and perhaps apicomplexans as a whole, because of their morphological and life history characteristics. In general, gregarines exhibit various forms of motility, which seem to depend on cytoskeletal structures (subpellicular microtubules, intermediate filaments, actin and myosin). Selenidiid archigregarines exhibit a pendular or rolling movement. Their surface, covered by a typical threemembrane pellicle displays broad folds, beneath each sets of parallel longitudinal subpellicular microtubules are present. In this study experiments using cytoskeletal drugs were performed on living individuals of Selenidium sp., parasitising the intestine of marine polychaete Pygospio elegans (Spionidae). Two probes, cytochalasine D and jasplakinolide, influencing the polymerisation of actin were applied to determine the role of actin filaments in motility. Furthermore, drugs that destroy existing microtubules and inhibit tubulin polymerization, oryzalin and colchicine, were used to confirm the role of subpellicular microtubules. Expected results of this study should verify the fundamental role of cytoskeletal proteins, actin and tubulin, in archigregarine motility and compare their movement mechanism to the substratedependent gliding machinery (= the so called “glideosome” concept) described in apicomplexan zoites. The authors acknowledge the financial support from a project No. GBP505/12/G112 (ECIP) from Czech Science Foundation, and Assoc. prof. T. G. Simdyanov for help with material collection.
ER  -

KOVÁČIKOVÁ, Magdaléna, Andrei DIAKIN, Gita G. PASKEROVA and Andrea BARDŮNEK VALIGUROVÁ. The motility in selenidiid archigregarines (Selenidiidae), an early emerging group of Apicomplexa. In \textit{12th European Multicolloquium of Parasitology}. 2016.

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