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@article{1354565,
author = {Vašková, Lucie and Nosková, Libuše and Bláhová, Barbora and Wimmerová, Michaela and Dřevínek, Pavel and Kubíčková, Božena and Stiborová, Marie and Hodek, Petr},
article_number = {5},
doi = {http://dx.doi.org/10.1007/s00706-016-1687-9},
keywords = {Receptors;Glycoproteins;Fluorescence spectroscopy;Cystic fibrosis;Antibacterial antibody},
language = {eng},
issn = {0026-9247},
journal = {Monatshefte für Chemie - Chemical Monthly},
title = {Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells},
volume = {147},
year = {2016}
}
TY - JOUR
ID - 1354565
AU - Vašková, Lucie - Nosková, Libuše - Bláhová, Barbora - Wimmerová, Michaela - Dřevínek, Pavel - Kubíčková, Božena - Stiborová, Marie - Hodek, Petr
PY - 2016
TI - Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells
JF - Monatshefte für Chemie - Chemical Monthly
VL - 147
IS - 5
SP - 889-896
EP - 889-896
SN - 00269247
KW - Receptors;Glycoproteins;Fluorescence spectroscopy;Cystic fibrosis;Antibacterial antibody
N2 - Hen yolk antibody (IgY) against Pseudomonasaeruginosa (PA) virulence factor, lectin PAIIL, was prepared from two hens. As judged from ELISA, both animals produced equally reactive anti-PAIIL antibodies. The IgY prophylaxis against adhesion of PA on epithelium cell lines derived from normal or cystic fibrosis (CF) human lungs was examined. Both anti-PAIIL antibodies comparably prevented PA adherence on epithelial cells. In accordance with clinical data, cells from CF patient showed higher susceptibility to PA binding compared to cells of a healthy subject. In additional experiments, a luminescent PA strain (PA-lux) was used in the PA adhesion assay instead of an originally used regular PA strain (ST 1763). The employment of PA-lux in the assay is advantageous since the bacteria do not need to be fluorescently labeled for their quantification. The application of both non-luminescent PA (ST 1763) and newly tested PA-lux strains provides consistent results as far as the anti-PAIIL IgY protection against of PA adherence is concerned. Furthermore, in lung epithelium adherence assay we examined also other PA strains for their sensitivity to anti-PAIIL antibody. The adherence of all PA strains tested was reduced by anti-PAIIL antibody when compared with un-affected controls. This finding suggests a wide applicability of anti-PAIIL antibody in prophylaxis of PA lung infections.
ER -
VAŠKOVÁ, Lucie, Libuše NOSKOVÁ, Barbora BLÁHOVÁ, Michaela WIMMEROVÁ, Pavel DŘEVÍNEK, Božena KUBÍČKOVÁ, Marie STIBOROVÁ a Petr HODEK. Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells. Online. \textit{Monatshefte für Chemie - Chemical Monthly}. 2016, roč.~147, č.~5, s.~889-896. ISSN~0026-9247. Dostupné z: https://dx.doi.org/10.1007/s00706-016-1687-9. [citováno 2024-04-24]
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