VAŠKOVÁ, Lucie, Libuše NOSKOVÁ, Barbora BLÁHOVÁ, Michaela WIMMEROVÁ, Pavel DŘEVÍNEK, Božena KUBÍČKOVÁ, Marie STIBOROVÁ and Petr HODEK. Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells. Monatshefte für Chemie - Chemical Monthly. 2016, vol. 147, No 5, p. 889-896. ISSN 0026-9247. Available from: https://dx.doi.org/10.1007/s00706-016-1687-9.
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Basic information
Original name Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells
Authors VAŠKOVÁ, Lucie (203 Czech Republic), Libuše NOSKOVÁ (203 Czech Republic), Barbora BLÁHOVÁ (203 Czech Republic), Michaela WIMMEROVÁ (203 Czech Republic, belonging to the institution), Pavel DŘEVÍNEK (203 Czech Republic), Božena KUBÍČKOVÁ (203 Czech Republic), Marie STIBOROVÁ (203 Czech Republic) and Petr HODEK (203 Czech Republic, guarantor).
Edition Monatshefte für Chemie - Chemical Monthly, 2016, 0026-9247.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Austria
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.282
RIV identification code RIV/00216224:14310/16:00090864
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s00706-016-1687-9
UT WoS 000374172200007
Keywords in English Receptors;Glycoproteins;Fluorescence spectroscopy;Cystic fibrosis;Antibacterial antibody
Tags AKR, cystic fibrosis, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 5/4/2017 18:19.
Abstract
Hen yolk antibody (IgY) against Pseudomonasaeruginosa (PA) virulence factor, lectin PAIIL, was prepared from two hens. As judged from ELISA, both animals produced equally reactive anti-PAIIL antibodies. The IgY prophylaxis against adhesion of PA on epithelium cell lines derived from normal or cystic fibrosis (CF) human lungs was examined. Both anti-PAIIL antibodies comparably prevented PA adherence on epithelial cells. In accordance with clinical data, cells from CF patient showed higher susceptibility to PA binding compared to cells of a healthy subject. In additional experiments, a luminescent PA strain (PA-lux) was used in the PA adhesion assay instead of an originally used regular PA strain (ST 1763). The employment of PA-lux in the assay is advantageous since the bacteria do not need to be fluorescently labeled for their quantification. The application of both non-luminescent PA (ST 1763) and newly tested PA-lux strains provides consistent results as far as the anti-PAIIL IgY protection against of PA adherence is concerned. Furthermore, in lung epithelium adherence assay we examined also other PA strains for their sensitivity to anti-PAIIL antibody. The adherence of all PA strains tested was reduced by anti-PAIIL antibody when compared with un-affected controls. This finding suggests a wide applicability of anti-PAIIL antibody in prophylaxis of PA lung infections.
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