J 2016

Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells

VAŠKOVÁ, Lucie, Libuše NOSKOVÁ, Barbora BLÁHOVÁ, Michaela WIMMEROVÁ, Pavel DŘEVÍNEK et. al.

Basic information

Original name

Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells

Authors

VAŠKOVÁ, Lucie (203 Czech Republic), Libuše NOSKOVÁ (203 Czech Republic), Barbora BLÁHOVÁ (203 Czech Republic), Michaela WIMMEROVÁ (203 Czech Republic, belonging to the institution), Pavel DŘEVÍNEK (203 Czech Republic), Božena KUBÍČKOVÁ (203 Czech Republic), Marie STIBOROVÁ (203 Czech Republic) and Petr HODEK (203 Czech Republic, guarantor)

Edition

Monatshefte für Chemie - Chemical Monthly, 2016, 0026-9247

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.282

RIV identification code

RIV/00216224:14310/16:00090864

Organization unit

Faculty of Science

DOI

UT WoS

000374172200007

Keywords in English

Receptors;Glycoproteins;Fluorescence spectroscopy;Cystic fibrosis;Antibacterial antibody

Tags

Tags

International impact, Reviewed
Změněno: 5/4/2017 18:19, Ing. Andrea Mikešková

Abstract

V originále

Hen yolk antibody (IgY) against Pseudomonasaeruginosa (PA) virulence factor, lectin PAIIL, was prepared from two hens. As judged from ELISA, both animals produced equally reactive anti-PAIIL antibodies. The IgY prophylaxis against adhesion of PA on epithelium cell lines derived from normal or cystic fibrosis (CF) human lungs was examined. Both anti-PAIIL antibodies comparably prevented PA adherence on epithelial cells. In accordance with clinical data, cells from CF patient showed higher susceptibility to PA binding compared to cells of a healthy subject. In additional experiments, a luminescent PA strain (PA-lux) was used in the PA adhesion assay instead of an originally used regular PA strain (ST 1763). The employment of PA-lux in the assay is advantageous since the bacteria do not need to be fluorescently labeled for their quantification. The application of both non-luminescent PA (ST 1763) and newly tested PA-lux strains provides consistent results as far as the anti-PAIIL IgY protection against of PA adherence is concerned. Furthermore, in lung epithelium adherence assay we examined also other PA strains for their sensitivity to anti-PAIIL antibody. The adherence of all PA strains tested was reduced by anti-PAIIL antibody when compared with un-affected controls. This finding suggests a wide applicability of anti-PAIIL antibody in prophylaxis of PA lung infections.