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@article{1354565, author = {Vašková, Lucie and Nosková, Libuše and Bláhová, Barbora and Wimmerová, Michaela and Dřevínek, Pavel and Kubíčková, Božena and Stiborová, Marie and Hodek, Petr}, article_number = {5}, doi = {http://dx.doi.org/10.1007/s00706-016-1687-9}, keywords = {Receptors;Glycoproteins;Fluorescence spectroscopy;Cystic fibrosis;Antibacterial antibody}, language = {eng}, issn = {0026-9247}, journal = {Monatshefte für Chemie - Chemical Monthly}, title = {Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells}, volume = {147}, year = {2016} }
TY - JOUR ID - 1354565 AU - Vašková, Lucie - Nosková, Libuše - Bláhová, Barbora - Wimmerová, Michaela - Dřevínek, Pavel - Kubíčková, Božena - Stiborová, Marie - Hodek, Petr PY - 2016 TI - Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells JF - Monatshefte für Chemie - Chemical Monthly VL - 147 IS - 5 SP - 889-896 EP - 889-896 SN - 00269247 KW - Receptors;Glycoproteins;Fluorescence spectroscopy;Cystic fibrosis;Antibacterial antibody N2 - Hen yolk antibody (IgY) against Pseudomonasaeruginosa (PA) virulence factor, lectin PAIIL, was prepared from two hens. As judged from ELISA, both animals produced equally reactive anti-PAIIL antibodies. The IgY prophylaxis against adhesion of PA on epithelium cell lines derived from normal or cystic fibrosis (CF) human lungs was examined. Both anti-PAIIL antibodies comparably prevented PA adherence on epithelial cells. In accordance with clinical data, cells from CF patient showed higher susceptibility to PA binding compared to cells of a healthy subject. In additional experiments, a luminescent PA strain (PA-lux) was used in the PA adhesion assay instead of an originally used regular PA strain (ST 1763). The employment of PA-lux in the assay is advantageous since the bacteria do not need to be fluorescently labeled for their quantification. The application of both non-luminescent PA (ST 1763) and newly tested PA-lux strains provides consistent results as far as the anti-PAIIL IgY protection against of PA adherence is concerned. Furthermore, in lung epithelium adherence assay we examined also other PA strains for their sensitivity to anti-PAIIL antibody. The adherence of all PA strains tested was reduced by anti-PAIIL antibody when compared with un-affected controls. This finding suggests a wide applicability of anti-PAIIL antibody in prophylaxis of PA lung infections. ER -
VAŠKOVÁ, Lucie, Libuše NOSKOVÁ, Barbora BLÁHOVÁ, Michaela WIMMEROVÁ, Pavel DŘEVÍNEK, Božena KUBÍČKOVÁ, Marie STIBOROVÁ and Petr HODEK. Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells. \textit{Monatshefte für Chemie - Chemical Monthly}. 2016, vol.~147, No~5, p.~889-896. ISSN~0026-9247. Available from: https://dx.doi.org/10.1007/s00706-016-1687-9.
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