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@article{1354613,
author = {Škoda, Jan and Ňuňuková, Alena and Loja, Tomáš and Zambo, Iva and Neradil, Jakub and Múdry, Peter and Zitterbart, Karel and Hermanová, Markéta and Hampl, Aleš and Štěrba, Jaroslav and Veselská, Renata},
article_location = {Dordrecht},
article_number = {7},
doi = {http://dx.doi.org/10.1007/s13277-016-4837-0},
keywords = {cancer stem cells;pediatric sarcomas;markers;tumorigenicity;Sox2},
language = {eng},
issn = {1010-4283},
journal = {Tumor Biology},
title = {Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice},
volume = {37},
year = {2016}
}
TY - JOUR
ID - 1354613
AU - Škoda, Jan - Ňuňuková, Alena - Loja, Tomáš - Zambo, Iva - Neradil, Jakub - Múdry, Peter - Zitterbart, Karel - Hermanová, Markéta - Hampl, Aleš - Štěrba, Jaroslav - Veselská, Renata
PY - 2016
TI - Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice
JF - Tumor Biology
VL - 37
IS - 7
SP - 9535-9548
EP - 9535-9548
PB - Springer Netherlands
SN - 10104283
KW - cancer stem cells;pediatric sarcomas;markers;tumorigenicity;Sox2
N2 - The three most frequent pediatric sarcomas, i.e., Ewing's sarcoma, osteosarcoma, and rhabdomyosarcoma, were examined in this study: three cell lines derived from three primary tumor samples were analyzed from each of these tumor types. Detailed comparative analysis of the expression of three putative cancer stem cell markers related to sarcomas-ABCG2, CD133, and nestin-was performed on both primary tumor tissues and corresponding cell lines. The obtained results showed that the frequency of ABCG2-positive and CD133-positive cells was predominantly increased in the respective cell lines but that the high levels of nestin expression were reduced in both osteosarcomas and rhabdomyosarcomas under in vitro conditions. These findings suggest the selection advantage of cells expressing ABCG2 or CD133, but the functional tests in NOD/SCID gamma mice did not confirm the tumorigenic potential of cells harboring this phenotype. Subsequent analysis of the expression of common stem cell markers revealed an evident relationship between the expression of the transcription factor Sox2 and the tumorigenicity of the cell lines in immunodeficient mice: the Sox2 levels were highest in the two cell lines that were demonstrated as tumorigenic. Furthermore, Sox2-positive cells were found in the respective primary tumors and all xenograft tumors showed apparent accumulation of these cells. All of these findings support our conclusion that regardless of the expression of ABCG2, CD133 and nestin, only cells displaying increased Sox2 expression are directly involved in tumor initiation and growth; therefore, these cells fit the definition of the cancer stem cell phenotype.
ER -
ŠKODA, Jan, Alena ŇUŇUKOVÁ, Tomáš LOJA, Iva ZAMBO, Jakub NERADIL, Peter MÚDRY, Karel ZITTERBART, Markéta HERMANOVÁ, Aleš HAMPL, Jaroslav ŠTĚRBA and Renata VESELSKÁ. Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice. \textit{Tumor Biology}. Dordrecht: Springer Netherlands, vol.~37, No~7, p.~9535-9548. ISSN~1010-4283. doi:10.1007/s13277-016-4837-0. 2016.
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