Cancer stem cell markers in pediatric sarcomas: Sox2 is
associated with tumorigenicity in immunodeficient mice

J 2016

Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice

ŠKODA, Jan, Alena ŇUŇUKOVÁ, Tomáš LOJA, Iva ZAMBO, Jakub NERADIL et. al.

Basic information

Original name

Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice

Authors

ŠKODA, Jan (203 Czech Republic, belonging to the institution), Alena ŇUŇUKOVÁ (703 Slovakia, belonging to the institution), Tomáš LOJA (703 Slovakia, belonging to the institution), Iva ZAMBO (203 Czech Republic, belonging to the institution), Jakub NERADIL (203 Czech Republic, belonging to the institution), Peter MÚDRY (203 Czech Republic, belonging to the institution), Karel ZITTERBART (203 Czech Republic, belonging to the institution), Markéta HERMANOVÁ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution), Jaroslav ŠTĚRBA (203 Czech Republic, belonging to the institution) and Renata VESELSKÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Tumor Biology, Dordrecht, Springer Netherlands, 2016, 1010-4283

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30209 Paediatrics

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.650

RIV identification code

RIV/00216224:14310/16:00088885

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s13277-016-4837-0

UT WoS

000382174500105

Keywords in English

cancer stem cells;pediatric sarcomas;markers;tumorigenicity;Sox2

Abstract

V originále

The three most frequent pediatric sarcomas, i.e., Ewing's sarcoma, osteosarcoma, and rhabdomyosarcoma, were examined in this study: three cell lines derived from three primary tumor samples were analyzed from each of these tumor types. Detailed comparative analysis of the expression of three putative cancer stem cell markers related to sarcomas-ABCG2, CD133, and nestin-was performed on both primary tumor tissues and corresponding cell lines. The obtained results showed that the frequency of ABCG2-positive and CD133-positive cells was predominantly increased in the respective cell lines but that the high levels of nestin expression were reduced in both osteosarcomas and rhabdomyosarcomas under in vitro conditions. These findings suggest the selection advantage of cells expressing ABCG2 or CD133, but the functional tests in NOD/SCID gamma mice did not confirm the tumorigenic potential of cells harboring this phenotype. Subsequent analysis of the expression of common stem cell markers revealed an evident relationship between the expression of the transcription factor Sox2 and the tumorigenicity of the cell lines in immunodeficient mice: the Sox2 levels were highest in the two cell lines that were demonstrated as tumorigenic. Furthermore, Sox2-positive cells were found in the respective primary tumors and all xenograft tumors showed apparent accumulation of these cells. All of these findings support our conclusion that regardless of the expression of ABCG2, CD133 and nestin, only cells displaying increased Sox2 expression are directly involved in tumor initiation and growth; therefore, these cells fit the definition of the cancer stem cell phenotype.

Links

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

NT13443, research and development project

Name: Identifikace a charakterizace nádorových kmenových buněk u sarkomů dětského věku.

Citovat

ŠKODA, Jan, Alena ŇUŇUKOVÁ, Tomáš LOJA, Iva ZAMBO, Jakub NERADIL, Peter MÚDRY, Karel ZITTERBART, Markéta HERMANOVÁ, Aleš HAMPL, Jaroslav ŠTĚRBA and Renata VESELSKÁ. Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice. Tumor Biology. Dordrecht: Springer Netherlands, 2016, vol. 37, No 7, p. 9535-9548. ISSN 1010-4283. Available from: https://dx.doi.org/10.1007/s13277-016-4837-0.

@article{1354613,
   author = {Škoda, Jan and Ňuňuková, Alena and Loja, Tomáš and Zambo, Iva and Neradil, Jakub and Múdry, Peter and Zitterbart, Karel and Hermanová, Markéta and Hampl, Aleš and Štěrba, Jaroslav and Veselská, Renata},
   article_location = {Dordrecht},
   article_number = {7},
   doi = {http://dx.doi.org/10.1007/s13277-016-4837-0},
   keywords = {cancer stem cells;pediatric sarcomas;markers;tumorigenicity;Sox2},
   language = {eng},
   issn = {1010-4283},
   journal = {Tumor Biology},
   title = {Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice},
   volume = {37},
   year = {2016}
}

TY  - JOUR
ID  - 1354613
AU  - Škoda, Jan - Ňuňuková, Alena - Loja, Tomáš - Zambo, Iva - Neradil, Jakub - Múdry, Peter - Zitterbart, Karel - Hermanová, Markéta - Hampl, Aleš - Štěrba, Jaroslav - Veselská, Renata
PY  - 2016
TI  - Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice
JF  - Tumor Biology
VL  - 37
IS  - 7
SP  - 9535-9548
EP  - 9535-9548
PB  - Springer Netherlands
SN  - 10104283
KW  - cancer stem cells;pediatric sarcomas;markers;tumorigenicity;Sox2
N2  - The three most frequent pediatric sarcomas, i.e., Ewing's sarcoma, osteosarcoma, and rhabdomyosarcoma, were examined in this study: three cell lines derived from three primary tumor samples were analyzed from each of these tumor types. Detailed comparative analysis of the expression of three putative cancer stem cell markers related to sarcomas-ABCG2, CD133, and nestin-was performed on both primary tumor tissues and corresponding cell lines. The obtained results showed that the frequency of ABCG2-positive and CD133-positive cells was predominantly increased in the respective cell lines but that the high levels of nestin expression were reduced in both osteosarcomas and rhabdomyosarcomas under in vitro conditions. These findings suggest the selection advantage of cells expressing ABCG2 or CD133, but the functional tests in NOD/SCID gamma mice did not confirm the tumorigenic potential of cells harboring this phenotype. Subsequent analysis of the expression of common stem cell markers revealed an evident relationship between the expression of the transcription factor Sox2 and the tumorigenicity of the cell lines in immunodeficient mice: the Sox2 levels were highest in the two cell lines that were demonstrated as tumorigenic. Furthermore, Sox2-positive cells were found in the respective primary tumors and all xenograft tumors showed apparent accumulation of these cells. All of these findings support our conclusion that regardless of the expression of ABCG2, CD133 and nestin, only cells displaying increased Sox2 expression are directly involved in tumor initiation and growth; therefore, these cells fit the definition of the cancer stem cell phenotype.
ER  -

ŠKODA, Jan, Alena ŇUŇUKOVÁ, Tomáš LOJA, Iva ZAMBO, Jakub NERADIL, Peter MÚDRY, Karel ZITTERBART, Markéta HERMANOVÁ, Aleš HAMPL, Jaroslav ŠTĚRBA and Renata VESELSKÁ. Cancer stem cell markers in pediatric sarcomas: Sox2 is associated with tumorigenicity in immunodeficient mice. \textit{Tumor Biology}. Dordrecht: Springer Netherlands, 2016, vol.~37, No~7, p.~9535-9548. ISSN~1010-4283. Available from: https://dx.doi.org/10.1007/s13277-016-4837-0.

Detailed Information on Publication Record