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@article{1354631,
author = {Ustianenko, Dmytro and Pasulka, Josef and Feketová, Zuzana and Bednařík, Lukáš and Zigáčková, Dagmar and Fořtová, Andrea and Zavolan, Mihaela and Vaňáčová, Štěpánka},
article_location = {Hoboken},
article_number = {20},
doi = {http://dx.doi.org/10.15252/embj.201694857},
keywords = {DIS3L2; RNA surveillance; TSSa; ncRNAs; uridylation},
language = {eng},
issn = {0261-4189},
journal = {EMBO JOURNAL},
title = {TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs},
url = {http://emboj.embopress.org/content/early/2016/09/19/embj.201694857},
volume = {35},
year = {2016}
}
TY - JOUR
ID - 1354631
AU - Ustianenko, Dmytro - Pasulka, Josef - Feketová, Zuzana - Bednařík, Lukáš - Zigáčková, Dagmar - Fořtová, Andrea - Zavolan, Mihaela - Vaňáčová, Štěpánka
PY - 2016
TI - TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs
JF - EMBO JOURNAL
VL - 35
IS - 20
SP - 2179-2191
EP - 2179-2191
PB - Wiley-Blackwell
SN - 02614189
KW - DIS3L2
KW - RNA surveillance
KW - TSSa
KW - ncRNAs
KW - uridylation
UR - http://emboj.embopress.org/content/early/2016/09/19/embj.201694857
L2 - http://emboj.embopress.org/content/early/2016/09/19/embj.201694857
N2 - Uridylation of various cellular RNA species at the 3' end has been generally linked to RNA degradation. In mammals, uridylated pre-let-7 miRNAs and mRNAs are targeted by the 3' to 5' exoribonuclease DIS3L2. Mutations in DIS3L2 have been associated with Perlman syndrome and with Wilms tumor susceptibility. Using in vivo cross-linking and immunoprecipitation (CLIP) method, we discovered the DIS3L2-dependent cytoplasmic uridylome of human cells. We found a broad spectrum of uridylated RNAs including rRNAs, snRNAs, snoRNAs, tRNAs, vault, 7SL, Y RNAs, mRNAs, lncRNAs, and transcripts from pseudogenes. The unifying features of most of these identified RNAs are aberrant processing and the presence of stable secondary structures. Most importantly, we demonstrate that uridylation mediates DIS3L2 degradation of short RNA polymerase II-derived RNAs. Our findings establish the role of DIS3L2 and oligouridylation as the cytoplasmic quality control for highly structured ncRNAs.
ER -
USTIANENKO, Dmytro, Josef PASULKA, Zuzana FEKETOVÁ, Lukáš BEDNAŘÍK, Dagmar ZIGÁČKOVÁ, Andrea FOŘTOVÁ, Mihaela ZAVOLAN and Štěpánka VAŇÁČOVÁ. TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs. \textit{EMBO JOURNAL}. Hoboken: Wiley-Blackwell, 2016, vol.~35, No~20, p.~2179-2191. ISSN~0261-4189. Available from: https://dx.doi.org/10.15252/embj.201694857.
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