Detailed Information on Publication Record
2016
Cytokinins control cell differentiation via regulation of secondary cell wall master switches NST1 and NST3
DIDI, Vojtěch, Jana VAŠÍČKOVÁ, Radek JUPA, Mariana BENITEZ KEINRAD, Tereza DOBISOVÁ et. al.Basic information
Original name
Cytokinins control cell differentiation via regulation of secondary cell wall master switches NST1 and NST3
Authors
DIDI, Vojtěch (203 Czech Republic, belonging to the institution), Jana VAŠÍČKOVÁ (203 Czech Republic), Radek JUPA (203 Czech Republic, belonging to the institution), Mariana BENITEZ KEINRAD (484 Mexico, belonging to the institution), Tereza DOBISOVÁ (203 Czech Republic, belonging to the institution), Anna BÍLKOVÁ (203 Czech Republic), Eva BUDINSKÁ (703 Slovakia, belonging to the institution), Radim ČEGAN (203 Czech Republic), Roman HOBZA (203 Czech Republic, belonging to the institution), Shawn MANSFIELD (124 Canada), Vít GLOSER (203 Czech Republic, belonging to the institution) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution)
Edition
Norwegian Plant Biology 2016, 2016
Other information
Language
English
Type of outcome
Prezentace na konferencích
Field of Study
Genetics and molecular biology
Country of publisher
Norway
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14740/16:00088172
Organization unit
Central European Institute of Technology
Keywords in English
Secondary cell wall; cytokinin
Změněno: 21/9/2016 12:56, Mgr. Vojtěch Didi, Ph.D.
Abstract
V originále
Plant hormones cytokinins were shown to control the equilibrium between cell division and cell differentiation, the process of key developmental importance across all kingdoms. However, the underlying molecular mechanism is mostly unknown. Here we present the role of cytokinins in the control of differentiation program in secondary cell wall (SCW) containing cells. We found that impaired cytokinin signaling disturbs apical/basal developmental gradient in the inflorescence stem of Arabidopsis thaliana. Attenuating cytokinin signaling results in precocious differentiation of SCW containing cells (vessels and fibers), higher lignin and sugars content and formation of vessels with reduced diameter and impaired water conductance. The next-gen transcriptional profiling of cytokinin signaling mutants revealed ectopic upregulation of NST1 and NST3, the NAC transcription factors and master switches of SCW formation and the regulators and effector proteins of the downstream transcriptional cascade. The negative role of cytokinins in the control over SCW formation via inhibiting NSTs expression was confirmed using exogenous cytokinin application in pNST3:NST3-GUS line and via genetic manipulation of endogenous cytokinin content in the cytokinin biosynthetic mutant atipt1,3,5,7. Importantly, exogenous cytokinin application reversed not only the early SCW formation phenotype of atipt1,3,5,7 plants, but also recovered the WT expression gradient of the entire SCW developmental pathway. Based on our findings we propose the role of cytokinins as a positional cue controlling cell differentiation status via regulating expression of SCW master switches NST1 and NST3.
Links
ED1.1.00/02.0068, research and development project |
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GA13-25280S, research and development project |
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7F14155, research and development project |
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