Neovahlkampfia nana n. sp Reinforcing an Underrepresented
Subclade of Tetramitia, Heterolobosea

J 2017

Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea

TYML, Tomáš, Luis Fernando LARES JIMENEZ, Martin KOSTKA and Iva DYKOVÁ

Basic information

Original name

Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea

Authors

TYML, Tomáš (203 Czech Republic, guarantor, belonging to the institution), Luis Fernando LARES JIMENEZ (484 Mexico, belonging to the institution), Martin KOSTKA (203 Czech Republic) and Iva DYKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Journal of Eukaryotic Microbiology, HOBOKEN, WILEY-BLACKWELL, 2017, 1066-5234

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.537

RIV identification code

RIV/00216224:14310/17:00094556

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/jeu.12341

UT WoS

000391930200007

Keywords in English

18S rRNA; Discicristata; free-living amebas; sequence variability; taxonomy; ultrastructure

Abstract

V originále

The study provides robust genetic evidence that a newly isolated naked ameba with morphological and ultrastructural features indicative of Heterolobosea is a new species. Neovahlkampfia nana n. sp. associates with the yet underrepresented subclade of Tetramitia I. Considerable differences found in 18S rRNA gene sequences of individual molecular clones derived from DNA of five clonal cultures, using a low fidelity DNA polymerase, raised the issue of intragenomic sequence variation, a phenomenon that has not been previously studied in Heterolobosea. However, as proved using a higher fidelity DNA polymerase, the sequence variability observed was introduced by PCR mediated by the low fidelity polymerase and fixed by molecular cloning. This points to the potentially dubious validity of some current nominal species of Heterolobosea that differ from one another in just one or two base positions.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

TYML, Tomáš, Luis Fernando LARES JIMENEZ, Martin KOSTKA and Iva DYKOVÁ. Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea. Journal of Eukaryotic Microbiology. HOBOKEN: WILEY-BLACKWELL, 2017, vol. 64, No 1, p. 78-87. ISSN 1066-5234. Available from: https://dx.doi.org/10.1111/jeu.12341.

@article{1354647,
   author = {Tyml, Tomáš and Lares Jimenez, Luis Fernando and Kostka, Martin and Dyková, Iva},
   article_location = {HOBOKEN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/jeu.12341},
   keywords = {18S rRNA; Discicristata; free-living amebas; sequence variability; taxonomy; ultrastructure},
   language = {eng},
   issn = {1066-5234},
   journal = {Journal of Eukaryotic Microbiology},
   title = {Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/full},
   volume = {64},
   year = {2017}
}

TY  - JOUR
ID  - 1354647
AU  - Tyml, Tomáš - Lares Jimenez, Luis Fernando - Kostka, Martin - Dyková, Iva
PY  - 2017
TI  - Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea
JF  - Journal of Eukaryotic Microbiology
VL  - 64
IS  - 1
SP  - 78-87
EP  - 78-87
PB  - WILEY-BLACKWELL
SN  - 10665234
KW  - 18S rRNA
KW  - Discicristata
KW  - free-living amebas
KW  - sequence variability
KW  - taxonomy
KW  - ultrastructure
UR  - http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/full
L2  - http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/full
N2  - The study provides robust genetic evidence that a newly isolated naked ameba with morphological and ultrastructural features indicative of Heterolobosea is a new species. Neovahlkampfia nana n. sp. associates with the yet underrepresented subclade of Tetramitia I. Considerable differences found in 18S rRNA gene sequences of individual molecular clones derived from DNA of five clonal cultures, using a low fidelity DNA polymerase, raised the issue of intragenomic sequence variation, a phenomenon that has not been previously studied in Heterolobosea. However, as proved using a higher fidelity DNA polymerase, the sequence variability observed was introduced by PCR mediated by the low fidelity polymerase and fixed by molecular cloning. This points to the potentially dubious validity of some current nominal species of Heterolobosea that differ from one another in just one or two base positions.
ER  -

TYML, Tomáš, Luis Fernando LARES JIMENEZ, Martin KOSTKA and Iva DYKOVÁ. Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea. \textit{Journal of Eukaryotic Microbiology}. HOBOKEN: WILEY-BLACKWELL, 2017, vol.~64, No~1, p.~78-87. ISSN~1066-5234. Available from: https://dx.doi.org/10.1111/jeu.12341.

Detailed Information on Publication Record