TYML, Tomáš, Luis Fernando LARES JIMENEZ, Martin KOSTKA and Iva DYKOVÁ. Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea. Journal of Eukaryotic Microbiology. HOBOKEN: WILEY-BLACKWELL, 2017, vol. 64, No 1, p. 78-87. ISSN 1066-5234. Available from: https://dx.doi.org/10.1111/jeu.12341. |
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@article{1354647, author = {Tyml, Tomáš and Lares Jimenez, Luis Fernando and Kostka, Martin and Dyková, Iva}, article_location = {HOBOKEN}, article_number = {1}, doi = {http://dx.doi.org/10.1111/jeu.12341}, keywords = {18S rRNA; Discicristata; free-living amebas; sequence variability; taxonomy; ultrastructure}, language = {eng}, issn = {1066-5234}, journal = {Journal of Eukaryotic Microbiology}, title = {Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea}, url = {http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/full}, volume = {64}, year = {2017} }
TY - JOUR ID - 1354647 AU - Tyml, Tomáš - Lares Jimenez, Luis Fernando - Kostka, Martin - Dyková, Iva PY - 2017 TI - Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea JF - Journal of Eukaryotic Microbiology VL - 64 IS - 1 SP - 78-87 EP - 78-87 PB - WILEY-BLACKWELL SN - 10665234 KW - 18S rRNA KW - Discicristata KW - free-living amebas KW - sequence variability KW - taxonomy KW - ultrastructure UR - http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/full L2 - http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/full N2 - The study provides robust genetic evidence that a newly isolated naked ameba with morphological and ultrastructural features indicative of Heterolobosea is a new species. Neovahlkampfia nana n. sp. associates with the yet underrepresented subclade of Tetramitia I. Considerable differences found in 18S rRNA gene sequences of individual molecular clones derived from DNA of five clonal cultures, using a low fidelity DNA polymerase, raised the issue of intragenomic sequence variation, a phenomenon that has not been previously studied in Heterolobosea. However, as proved using a higher fidelity DNA polymerase, the sequence variability observed was introduced by PCR mediated by the low fidelity polymerase and fixed by molecular cloning. This points to the potentially dubious validity of some current nominal species of Heterolobosea that differ from one another in just one or two base positions. ER -
TYML, Tomáš, Luis Fernando LARES JIMENEZ, Martin KOSTKA and Iva DYKOVÁ. Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea. \textit{Journal of Eukaryotic Microbiology}. HOBOKEN: WILEY-BLACKWELL, 2017, vol.~64, No~1, p.~78-87. ISSN~1066-5234. Available from: https://dx.doi.org/10.1111/jeu.12341.
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