Chlamydial seasonal dynamics and isolation of ‘Candidatus
Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake.

J 2016

Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake.

PIZZETTI, Ilaria, Frederik SCHULZ, Tomáš TYML, Bernhard M. FUCHS, Rudolf AMANN et. al.

Basic information

Original name

Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake.

Authors

PIZZETTI, Ilaria (380 Italy), Frederik SCHULZ (40 Austria), Tomáš TYML (203 Czech Republic, guarantor, belonging to the institution), Bernhard M. FUCHS (276 Germany), Rudolf AMANN (276 Germany), Matthias HORN (40 Austria) and Stefano FAZI (380 Italy)

Edition

Environmental Microbiology, Hoboken, WILEY-BLACKWELL, 2016, 1462-2912

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.395

RIV identification code

RIV/00216224:14310/16:00094558

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/1462-2920.13111

UT WoS

000383388800014

Keywords in English

BAYESIAN PHYLOGENETIC INFERENCE; TARGETED OLIGONUCLEOTIDE PROBES; IN-SITU HYBRIDIZATION; WATER-TREATMENT-PLANT; ABORTED BOVINE FETUS; RIBOSOMAL-RNA GENE; ORDER CHLAMYDIALES; ENVIRONMENTAL CHLAMYDIAE; INTRACELLULAR BACTERIA; FAMILY CHLAMYDIACEAE

Abstract

V originále

The Chlamydiae are a phylum of obligate intracellular bacteria comprising important human and animal pathogens, yet their occurrence in the environment, their phylogenetic diversity and their host range has been largely underestimated. We investigated the seasonality of environmental chlamydiae in a Tyrrhenian coastal lake. By catalysed reporter deposition fluorescence in situ hybridization, we quantified the small planktonic cells and detected a peak in the abundance of environmental chlamydiae in early autumn with up to 5.9x10(4) cells ml(-1). Super-resolution microscopy improved the visualization and quantification of these bacteria and enabled the detection of pleomorphic chlamydial cells in their protist host directly in an environmental sample. To isolate environmental chlamydiae together with their host, we applied a high-throughput limited dilution approach and successfully recovered a Vexillifera sp., strain harbouring chlamydiae (93% 16S rRNA sequence identity to Simkania negevensis), tentatively named CandidatusNeptunochlamydia vexilliferae'. Transmission electron microscopy in combination with fluorescence in situ hybridization was used to prove the intracellular location of these bacteria representing the first strain of marine chlamydiae stably maintained alongside with their host in a laboratory culture. Taken together, this study contributes to a better understanding of the distribution and diversity of environmental chlamydiae in previously neglected marine environments.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

PIZZETTI, Ilaria, Frederik SCHULZ, Tomáš TYML, Bernhard M. FUCHS, Rudolf AMANN, Matthias HORN and Stefano FAZI. Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake. Environmental Microbiology. Hoboken: WILEY-BLACKWELL, 2016, vol. 18, No 8, p. 2405-2417. ISSN 1462-2912. Available from: https://dx.doi.org/10.1111/1462-2920.13111.

@article{1354651,
   author = {Pizzetti, Ilaria and Schulz, Frederik and Tyml, Tomáš and Fuchs, Bernhard M. and Amann, Rudolf and Horn, Matthias and Fazi, Stefano},
   article_location = {Hoboken},
   article_number = {8},
   doi = {http://dx.doi.org/10.1111/1462-2920.13111},
   keywords = {BAYESIAN PHYLOGENETIC INFERENCE; TARGETED OLIGONUCLEOTIDE PROBES; IN-SITU HYBRIDIZATION; WATER-TREATMENT-PLANT; ABORTED BOVINE FETUS; RIBOSOMAL-RNA GENE; ORDER CHLAMYDIALES; ENVIRONMENTAL CHLAMYDIAE; INTRACELLULAR BACTERIA; FAMILY CHLAMYDIACEAE},
   language = {eng},
   issn = {1462-2912},
   journal = {Environmental Microbiology},
   title = {Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake.},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13111/full},
   volume = {18},
   year = {2016}
}

TY  - JOUR
ID  - 1354651
AU  - Pizzetti, Ilaria - Schulz, Frederik - Tyml, Tomáš - Fuchs, Bernhard M. - Amann, Rudolf - Horn, Matthias - Fazi, Stefano
PY  - 2016
TI  - Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake.
JF  - Environmental Microbiology
VL  - 18
IS  - 8
SP  - 2405-2417
EP  - 2405-2417
PB  - WILEY-BLACKWELL
SN  - 14622912
KW  - BAYESIAN PHYLOGENETIC INFERENCE
KW  - TARGETED OLIGONUCLEOTIDE PROBES
KW  - IN-SITU HYBRIDIZATION
KW  - WATER-TREATMENT-PLANT
KW  - ABORTED BOVINE FETUS
KW  - RIBOSOMAL-RNA GENE
KW  - ORDER CHLAMYDIALES
KW  - ENVIRONMENTAL CHLAMYDIAE
KW  - INTRACELLULAR BACTERIA
KW  - FAMILY CHLAMYDIACEAE
UR  - http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13111/full
N2  - The Chlamydiae are a phylum of obligate intracellular bacteria comprising important human and animal pathogens, yet their occurrence in the environment, their phylogenetic diversity and their host range has been largely underestimated. We investigated the seasonality of environmental chlamydiae in a Tyrrhenian coastal lake. By catalysed reporter deposition fluorescence in situ hybridization, we quantified the small planktonic cells and detected a peak in the abundance of environmental chlamydiae in early autumn with up to 5.9x10(4) cells ml(-1). Super-resolution microscopy improved the visualization and quantification of these bacteria and enabled the detection of pleomorphic chlamydial cells in their protist host directly in an environmental sample. To isolate environmental chlamydiae together with their host, we applied a high-throughput limited dilution approach and successfully recovered a Vexillifera sp., strain harbouring chlamydiae (93% 16S rRNA sequence identity to Simkania negevensis), tentatively named CandidatusNeptunochlamydia vexilliferae'. Transmission electron microscopy in combination with fluorescence in situ hybridization was used to prove the intracellular location of these bacteria representing the first strain of marine chlamydiae stably maintained alongside with their host in a laboratory culture. Taken together, this study contributes to a better understanding of the distribution and diversity of environmental chlamydiae in previously neglected marine environments.
ER  -

PIZZETTI, Ilaria, Frederik SCHULZ, Tomáš TYML, Bernhard M. FUCHS, Rudolf AMANN, Matthias HORN and Stefano FAZI. Chlamydial seasonal dynamics and isolation of ‘Candidatus Neptunochlamydia vexilliferae’from a Tyrrhenian coastal lake. \textit{Environmental Microbiology}. Hoboken: WILEY-BLACKWELL, 2016, vol.~18, No~8, p.~2405-2417. ISSN~1462-2912. Available from: https://dx.doi.org/10.1111/1462-2920.13111.

Detailed Information on Publication Record