| AMBROŽOVÁ, Gabriela, Táňa FIDLEROVÁ, Hana VEREŠČÁKOVÁ, Adolf KOUDELKA, T.K. RUDOLPH, S.R. WOODCOCK, B.A. FREEMAN, Lukáš KUBALA and Michaela PEKAROVÁ. Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. AMSTERDAM: ELSEVIER SCIENCE BV, 2016, vol. 1860, No 11, p. 2428-2437. ISSN 0304-4165. Available from: https://dx.doi.org/10.1016/j.bbagen.2016.07.010. |
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@article{1355491,
author = {Ambrožová, Gabriela and Fidlerová, Táňa and Vereščáková, Hana and Koudelka, Adolf and Rudolph, T.K. and Woodcock, S.R. and Freeman, B.A. and Kubala, Lukáš and Pekarová, Michaela},
article_location = {AMSTERDAM},
article_number = {11},
doi = {http://dx.doi.org/10.1016/j.bbagen.2016.07.010},
keywords = {Nitro-fatty acids; Nitro-oleic acid; Endothelial cells; Macrophages; Vascular inflammation; Endothelial-mesenchymal transition},
language = {eng},
issn = {0304-4165},
journal = {BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS},
title = {Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition},
url = {https://www.ncbi.nlm.nih.gov/pubmed/27431604},
volume = {1860},
year = {2016}
}
TY - JOUR
ID - 1355491
AU - Ambrožová, Gabriela - Fidlerová, Táňa - Vereščáková, Hana - Koudelka, Adolf - Rudolph, T.K. - Woodcock, S.R. - Freeman, B.A. - Kubala, Lukáš - Pekarová, Michaela
PY - 2016
TI - Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition
JF - BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
VL - 1860
IS - 11
SP - 2428-2437
EP - 2428-2437
PB - ELSEVIER SCIENCE BV
SN - 03044165
KW - Nitro-fatty acids
KW - Nitro-oleic acid
KW - Endothelial cells
KW - Macrophages
KW - Vascular inflammation
KW - Endothelial-mesenchymal transition
UR - https://www.ncbi.nlm.nih.gov/pubmed/27431604
L2 - https://www.ncbi.nlm.nih.gov/pubmed/27431604
N2 - Background: Inflammatory-mediated pathological processes in the endothelium arise as a consequence of the dysregulation of vascular homeostasis. Of particular importance are mediators produced by stimulated monocytes/macrophages inducing activation of endothelial cells (ECs). This is manifested by excessive soluble pro-inflammatory mediator production and cell surface adhesion molecule expression. Nitro-fatty acids are endogenous products of metabolic and inflammatory reactions that display immuno-regulatory potential and may represent a novel therapeutic strategy to treat inflammatory diseases. The purpose of our study was to characterize the effects of nitro-oleic acid (OA-NO2) on inflammatory responses and the endothelial-mesenchymal transition (EndMT) in ECs that is a consequence of the altered healing phase of the immune response. Methods: The effect of OA-NO2 on inflammatory responses and EndMT was determined in murine macrophages and murine and human ECs using Western blotting, ELISA, immunostaining, and functional assays. Results: OA-NO2 limited the activation of macrophages and ECs by reducing pro-inflammatory cytokine production and adhesion molecule expression through its modulation of STAT. MAPK and NF-kappa B-regulated signaling. OA-NO2 also decreased transforming growth factor-beta-stimulated EndMT and pro-fibrotic phenotype of ECs. These effects are related to the downregulation of Smad2/3. Conclusions: The study shows the pleiotropic effect of OA-NO2 on regulating EC-macrophage interactions during the immune response and suggests a role for OA-NO2 in the regulation of vascular endothelial immune and fibrotic responses arising during chronic inflammation. General significance: These findings propose the OA-NO2 may be useful as a novel therapeutic agent for treatment of cardiovascular disorders associated with dysregulation of the endothelial immune response. (C) 2016 Elsevier B.V. All rights reserved.
ER -
AMBROŽOVÁ, Gabriela, Táňa FIDLEROVÁ, Hana VEREŠČÁKOVÁ, Adolf KOUDELKA, T.K. RUDOLPH, S.R. WOODCOCK, B.A. FREEMAN, Lukáš KUBALA and Michaela PEKAROVÁ. Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition. \textit{BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS}. AMSTERDAM: ELSEVIER SCIENCE BV, 2016, vol.~1860, No~11, p.~2428-2437. ISSN~0304-4165. Available from: https://dx.doi.org/10.1016/j.bbagen.2016.07.010.
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