Podrobný výpis o publikaci

AMBROŽOVÁ, Gabriela, Táňa FIDLEROVÁ, Hana VEREŠČÁKOVÁ, Adolf KOUDELKA, T.K. RUDOLPH, S.R. WOODCOCK, B.A. FREEMAN, Lukáš KUBALA a Michaela PEKAROVÁ. Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. AMSTERDAM: ELSEVIER SCIENCE BV, 2016, roč. 1860, č. 11, s. 2428-2437. ISSN 0304-4165. Dostupné z: https://dx.doi.org/10.1016/j.bbagen.2016.07.010.

Další formáty: BibTeX LaTeX RIS

TY  - JOUR
ID  - 1355491
AU  - Ambrožová, Gabriela - Fidlerová, Táňa - Vereščáková, Hana - Koudelka, Adolf - Rudolph, T.K. - Woodcock, S.R. - Freeman, B.A. - Kubala, Lukáš - Pekarová, Michaela
PY  - 2016
TI  - Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition
JF  - BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
VL  - 1860
IS  - 11
SP  - 2428-2437
EP  - 2428-2437
PB  - ELSEVIER SCIENCE BV
SN  - 03044165
KW  - Nitro-fatty acids
KW  - Nitro-oleic acid
KW  - Endothelial cells
KW  - Macrophages
KW  - Vascular inflammation
KW  - Endothelial-mesenchymal transition
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27431604
L2  - https://www.ncbi.nlm.nih.gov/pubmed/27431604
N2  - Background: Inflammatory-mediated pathological processes in the endothelium arise as a consequence of the dysregulation of vascular homeostasis. Of particular importance are mediators produced by stimulated monocytes/macrophages inducing activation of endothelial cells (ECs). This is manifested by excessive soluble pro-inflammatory mediator production and cell surface adhesion molecule expression. Nitro-fatty acids are endogenous products of metabolic and inflammatory reactions that display immuno-regulatory potential and may represent a novel therapeutic strategy to treat inflammatory diseases. The purpose of our study was to characterize the effects of nitro-oleic acid (OA-NO2) on inflammatory responses and the endothelial-mesenchymal transition (EndMT) in ECs that is a consequence of the altered healing phase of the immune response. Methods: The effect of OA-NO2 on inflammatory responses and EndMT was determined in murine macrophages and murine and human ECs using Western blotting, ELISA, immunostaining, and functional assays. Results: OA-NO2 limited the activation of macrophages and ECs by reducing pro-inflammatory cytokine production and adhesion molecule expression through its modulation of STAT. MAPK and NF-kappa B-regulated signaling. OA-NO2 also decreased transforming growth factor-beta-stimulated EndMT and pro-fibrotic phenotype of ECs. These effects are related to the downregulation of Smad2/3. Conclusions: The study shows the pleiotropic effect of OA-NO2 on regulating EC-macrophage interactions during the immune response and suggests a role for OA-NO2 in the regulation of vascular endothelial immune and fibrotic responses arising during chronic inflammation. General significance: These findings propose the OA-NO2 may be useful as a novel therapeutic agent for treatment of cardiovascular disorders associated with dysregulation of the endothelial immune response. (C) 2016 Elsevier B.V. All rights reserved.
ER  -

Základní údaje
Originální název	Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition
Autoři	AMBROŽOVÁ, Gabriela (203 Česká republika), Táňa FIDLEROVÁ (203 Česká republika), Hana VEREŠČÁKOVÁ (203 Česká republika, domácí), Adolf KOUDELKA (203 Česká republika, domácí), T.K. RUDOLPH (203 Česká republika), S.R. WOODCOCK (840 Spojené státy), B.A. FREEMAN (840 Spojené státy), Lukáš KUBALA (203 Česká republika, domácí) a Michaela PEKAROVÁ (703 Slovensko, garant, domácí).
Vydání	BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, AMSTERDAM, ELSEVIER SCIENCE BV, 2016, 0304-4165.

Další údaje
Originální jazyk	angličtina
Typ výsledku	Článek v odborném periodiku
Obor	10601 Cell biology
Stát vydavatele	Nizozemské království
Utajení	není předmětem státního či obchodního tajemství
WWW	URL
Impakt faktor	Impact factor: 4.702
Kód RIV	RIV/00216224:14310/16:00095883
Organizační jednotka	Přírodovědecká fakulta
Doi	http://dx.doi.org/10.1016/j.bbagen.2016.07.010
UT WoS	000383824900009
Klíčová slova anglicky	Nitro-fatty acids; Nitro-oleic acid; Endothelial cells; Macrophages; Vascular inflammation; Endothelial-mesenchymal transition
Štítky	NZ, rivok
Příznaky	Mezinárodní význam, Recenzováno
Změnil	Změnila: Ing. Nicole Zrilić, učo 240776. Změněno: 13. 4. 2018 14:42.

Anotace
Background: Inflammatory-mediated pathological processes in the endothelium arise as a consequence of the dysregulation of vascular homeostasis. Of particular importance are mediators produced by stimulated monocytes/macrophages inducing activation of endothelial cells (ECs). This is manifested by excessive soluble pro-inflammatory mediator production and cell surface adhesion molecule expression. Nitro-fatty acids are endogenous products of metabolic and inflammatory reactions that display immuno-regulatory potential and may represent a novel therapeutic strategy to treat inflammatory diseases. The purpose of our study was to characterize the effects of nitro-oleic acid (OA-NO2) on inflammatory responses and the endothelial-mesenchymal transition (EndMT) in ECs that is a consequence of the altered healing phase of the immune response. Methods: The effect of OA-NO2 on inflammatory responses and EndMT was determined in murine macrophages and murine and human ECs using Western blotting, ELISA, immunostaining, and functional assays. Results: OA-NO2 limited the activation of macrophages and ECs by reducing pro-inflammatory cytokine production and adhesion molecule expression through its modulation of STAT. MAPK and NF-kappa B-regulated signaling. OA-NO2 also decreased transforming growth factor-beta-stimulated EndMT and pro-fibrotic phenotype of ECs. These effects are related to the downregulation of Smad2/3. Conclusions: The study shows the pleiotropic effect of OA-NO2 on regulating EC-macrophage interactions during the immune response and suggests a role for OA-NO2 in the regulation of vascular endothelial immune and fibrotic responses arising during chronic inflammation. General significance: These findings propose the OA-NO2 may be useful as a novel therapeutic agent for treatment of cardiovascular disorders associated with dysregulation of the endothelial immune response. (C) 2016 Elsevier B.V. All rights reserved.

Anotace

Background: Inflammatory-mediated pathological processes in the endothelium arise as a consequence of the dysregulation of vascular homeostasis. Of particular importance are mediators produced by stimulated monocytes/macrophages inducing activation of endothelial cells (ECs). This is manifested by excessive soluble pro-inflammatory mediator production and cell surface adhesion molecule expression. Nitro-fatty acids are endogenous products of metabolic and inflammatory reactions that display immuno-regulatory potential and may represent a novel therapeutic strategy to treat inflammatory diseases. The purpose of our study was to characterize the effects of nitro-oleic acid (OA-NO2) on inflammatory responses and the endothelial-mesenchymal transition (EndMT) in ECs that is a consequence of the altered healing phase of the immune response. Methods: The effect of OA-NO2 on inflammatory responses and EndMT was determined in murine macrophages and murine and human ECs using Western blotting, ELISA, immunostaining, and functional assays. Results: OA-NO2 limited the activation of macrophages and ECs by reducing pro-inflammatory cytokine production and adhesion molecule expression through its modulation of STAT. MAPK and NF-kappa B-regulated signaling. OA-NO2 also decreased transforming growth factor-beta-stimulated EndMT and pro-fibrotic phenotype of ECs. These effects are related to the downregulation of Smad2/3. Conclusions: The study shows the pleiotropic effect of OA-NO2 on regulating EC-macrophage interactions during the immune response and suggests a role for OA-NO2 in the regulation of vascular endothelial immune and fibrotic responses arising during chronic inflammation. General significance: These findings propose the OA-NO2 may be useful as a novel therapeutic agent for treatment of cardiovascular disorders associated with dysregulation of the endothelial immune response. (C) 2016 Elsevier B.V. All rights reserved.