Nitro-oleic acid inhibits vascular endothelial inflammatory
responses and the endothelial-mesenchymal transition

J 2016

Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition

AMBROŽOVÁ, Gabriela, Táňa FIDLEROVÁ, Hana VEREŠČÁKOVÁ, Adolf KOUDELKA, T.K. RUDOLPH et. al.

Basic information

Original name

Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition

Authors

AMBROŽOVÁ, Gabriela (203 Czech Republic), Táňa FIDLEROVÁ (203 Czech Republic), Hana VEREŠČÁKOVÁ (203 Czech Republic, belonging to the institution), Adolf KOUDELKA (203 Czech Republic, belonging to the institution), T.K. RUDOLPH (203 Czech Republic), S.R. WOODCOCK (840 United States of America), B.A. FREEMAN (840 United States of America), Lukáš KUBALA (203 Czech Republic, belonging to the institution) and Michaela PEKAROVÁ (703 Slovakia, guarantor, belonging to the institution)

Edition

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, AMSTERDAM, ELSEVIER SCIENCE BV, 2016, 0304-4165

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.702

RIV identification code

RIV/00216224:14310/16:00095883

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.bbagen.2016.07.010

UT WoS

000383824900009

Keywords in English

Nitro-fatty acids; Nitro-oleic acid; Endothelial cells; Macrophages; Vascular inflammation; Endothelial-mesenchymal transition

Abstract

V originále

Background: Inflammatory-mediated pathological processes in the endothelium arise as a consequence of the dysregulation of vascular homeostasis. Of particular importance are mediators produced by stimulated monocytes/macrophages inducing activation of endothelial cells (ECs). This is manifested by excessive soluble pro-inflammatory mediator production and cell surface adhesion molecule expression. Nitro-fatty acids are endogenous products of metabolic and inflammatory reactions that display immuno-regulatory potential and may represent a novel therapeutic strategy to treat inflammatory diseases. The purpose of our study was to characterize the effects of nitro-oleic acid (OA-NO2) on inflammatory responses and the endothelial-mesenchymal transition (EndMT) in ECs that is a consequence of the altered healing phase of the immune response. Methods: The effect of OA-NO2 on inflammatory responses and EndMT was determined in murine macrophages and murine and human ECs using Western blotting, ELISA, immunostaining, and functional assays. Results: OA-NO2 limited the activation of macrophages and ECs by reducing pro-inflammatory cytokine production and adhesion molecule expression through its modulation of STAT. MAPK and NF-kappa B-regulated signaling. OA-NO2 also decreased transforming growth factor-beta-stimulated EndMT and pro-fibrotic phenotype of ECs. These effects are related to the downregulation of Smad2/3. Conclusions: The study shows the pleiotropic effect of OA-NO2 on regulating EC-macrophage interactions during the immune response and suggests a role for OA-NO2 in the regulation of vascular endothelial immune and fibrotic responses arising during chronic inflammation. General significance: These findings propose the OA-NO2 may be useful as a novel therapeutic agent for treatment of cardiovascular disorders associated with dysregulation of the endothelial immune response. (C) 2016 Elsevier B.V. All rights reserved.

Citovat

AMBROŽOVÁ, Gabriela, Táňa FIDLEROVÁ, Hana VEREŠČÁKOVÁ, Adolf KOUDELKA, T.K. RUDOLPH, S.R. WOODCOCK, B.A. FREEMAN, Lukáš KUBALA and Michaela PEKAROVÁ. Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. AMSTERDAM: ELSEVIER SCIENCE BV, 2016, vol. 1860, No 11, p. 2428-2437. ISSN 0304-4165. Available from: https://dx.doi.org/10.1016/j.bbagen.2016.07.010.

@article{1355491,
   author = {Ambrožová, Gabriela and Fidlerová, Táňa and Vereščáková, Hana and Koudelka, Adolf and Rudolph, T.K. and Woodcock, S.R. and Freeman, B.A. and Kubala, Lukáš and Pekarová, Michaela},
   article_location = {AMSTERDAM},
   article_number = {11},
   doi = {http://dx.doi.org/10.1016/j.bbagen.2016.07.010},
   keywords = {Nitro-fatty acids; Nitro-oleic acid; Endothelial cells; Macrophages; Vascular inflammation; Endothelial-mesenchymal transition},
   language = {eng},
   issn = {0304-4165},
   journal = {BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS},
   title = {Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition},
   url = {https://www.ncbi.nlm.nih.gov/pubmed/27431604},
   volume = {1860},
   year = {2016}
}

TY  - JOUR
ID  - 1355491
AU  - Ambrožová, Gabriela - Fidlerová, Táňa - Vereščáková, Hana - Koudelka, Adolf - Rudolph, T.K. - Woodcock, S.R. - Freeman, B.A. - Kubala, Lukáš - Pekarová, Michaela
PY  - 2016
TI  - Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition
JF  - BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
VL  - 1860
IS  - 11
SP  - 2428-2437
EP  - 2428-2437
PB  - ELSEVIER SCIENCE BV
SN  - 03044165
KW  - Nitro-fatty acids
KW  - Nitro-oleic acid
KW  - Endothelial cells
KW  - Macrophages
KW  - Vascular inflammation
KW  - Endothelial-mesenchymal transition
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27431604
L2  - https://www.ncbi.nlm.nih.gov/pubmed/27431604
N2  - Background: Inflammatory-mediated pathological processes in the endothelium arise as a consequence of the dysregulation of vascular homeostasis. Of particular importance are mediators produced by stimulated monocytes/macrophages inducing activation of endothelial cells (ECs). This is manifested by excessive soluble pro-inflammatory mediator production and cell surface adhesion molecule expression. Nitro-fatty acids are endogenous products of metabolic and inflammatory reactions that display immuno-regulatory potential and may represent a novel therapeutic strategy to treat inflammatory diseases. The purpose of our study was to characterize the effects of nitro-oleic acid (OA-NO2) on inflammatory responses and the endothelial-mesenchymal transition (EndMT) in ECs that is a consequence of the altered healing phase of the immune response. Methods: The effect of OA-NO2 on inflammatory responses and EndMT was determined in murine macrophages and murine and human ECs using Western blotting, ELISA, immunostaining, and functional assays. Results: OA-NO2 limited the activation of macrophages and ECs by reducing pro-inflammatory cytokine production and adhesion molecule expression through its modulation of STAT. MAPK and NF-kappa B-regulated signaling. OA-NO2 also decreased transforming growth factor-beta-stimulated EndMT and pro-fibrotic phenotype of ECs. These effects are related to the downregulation of Smad2/3. Conclusions: The study shows the pleiotropic effect of OA-NO2 on regulating EC-macrophage interactions during the immune response and suggests a role for OA-NO2 in the regulation of vascular endothelial immune and fibrotic responses arising during chronic inflammation. General significance: These findings propose the OA-NO2 may be useful as a novel therapeutic agent for treatment of cardiovascular disorders associated with dysregulation of the endothelial immune response. (C) 2016 Elsevier B.V. All rights reserved.
ER  -

AMBROŽOVÁ, Gabriela, Táňa FIDLEROVÁ, Hana VEREŠČÁKOVÁ, Adolf KOUDELKA, T.K. RUDOLPH, S.R. WOODCOCK, B.A. FREEMAN, Lukáš KUBALA and Michaela PEKAROVÁ. Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition. \textit{BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS}. AMSTERDAM: ELSEVIER SCIENCE BV, 2016, vol.~1860, No~11, p.~2428-2437. ISSN~0304-4165. Available from: https://dx.doi.org/10.1016/j.bbagen.2016.07.010.

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