Increased frequency of chromosome congression defects and
aneuploidy in mouse oocytes cultured at lower temperature

J 2017

Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature

DANADOVÁ, Jitka, Natalie MATIJESCUKOVA, Anna Mac Gillavry DANYLEVSKA and Martin ANGER

Basic information

Original name

Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature

Authors

DANADOVÁ, Jitka, Natalie MATIJESCUKOVA, Anna Mac Gillavry DANYLEVSKA and Martin ANGER

Edition

Reproduction, Fertility and Development, Clayton, CSIRO Publishing, 2017, 1031-3613

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Australia

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.105

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1071/RD15306

UT WoS

000399309500012

Keywords in English

anaphase-promoting complex/cyclosome; chromosome misalignment; culture temperature; IVM

Abstract

V originále

Optimal culture conditions are essential for successful IVM of mammalian oocytes and for their further development into an embryo. In the present study we used live cell imaging microscopy to assess the effects of suboptimal culture temperature on various aspects of IVM, including duration of meiosis I, dynamics of polar body extrusion, chromosome congression, anaphase-promoting complex/cyclosome (APC/C) activation and aneuploidy. The data showed that even a small deviation from the optimal incubation temperature causes marked changes in the duration and synchronicity of meiosis, APC/C activity and the frequency of chromosome congression and segregation errors. In vitro manipulation and maturation of germ cells is widely used in both human and animal artificial reproduction techniques. Mammalian oocytes are naturally prone to chromosomal segregation errors, which are responsible for severe mental and developmental disorders. The data presented herein demonstrate that exposure of mouse oocytes to suboptimal temperature during manipulation and maturation could further increase the frequency of chromosome segregation defects in these cells.

Links

GA15-04844S, research and development project

Name: Osud aneuploidních buněk ve vyvíjejícím se embryu a populaci kmenových buněk

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

DANADOVÁ, Jitka, Natalie MATIJESCUKOVA, Anna Mac Gillavry DANYLEVSKA and Martin ANGER. Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature. Reproduction, Fertility and Development. Clayton: CSIRO Publishing, 2017, vol. 29, No 5, p. 968-974. ISSN 1031-3613. Available from: https://dx.doi.org/10.1071/RD15306.

@article{1355521,
   author = {Danadová, Jitka and Matijescukova, Natalie and Danylevska, Anna Mac Gillavry and Anger, Martin},
   article_location = {Clayton},
   article_number = {5},
   doi = {http://dx.doi.org/10.1071/RD15306},
   keywords = {anaphase-promoting complex/cyclosome; chromosome misalignment; culture temperature; IVM},
   language = {eng},
   issn = {1031-3613},
   journal = {Reproduction, Fertility and Development},
   title = {Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature},
   url = {http://dx.doi.org/10.1071/RD15306},
   volume = {29},
   year = {2017}
}

TY  - JOUR
ID  - 1355521
AU  - Danadová, Jitka - Matijescukova, Natalie - Danylevska, Anna Mac Gillavry - Anger, Martin
PY  - 2017
TI  - Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature
JF  - Reproduction, Fertility and Development
VL  - 29
IS  - 5
SP  - 968-974
EP  - 968-974
PB  - CSIRO Publishing
SN  - 10313613
KW  - anaphase-promoting complex/cyclosome
KW  - chromosome misalignment
KW  - culture temperature
KW  - IVM
UR  - http://dx.doi.org/10.1071/RD15306
L2  - http://dx.doi.org/10.1071/RD15306
N2  - Optimal culture conditions are essential for successful IVM of mammalian oocytes and for their further development into an embryo. In the present study we used live cell imaging microscopy to assess the effects of suboptimal culture temperature on various aspects of IVM, including duration of meiosis I, dynamics of polar body extrusion, chromosome congression, anaphase-promoting complex/cyclosome (APC/C) activation and aneuploidy. The data showed that even a small deviation from the optimal incubation temperature causes marked changes in the duration and synchronicity of meiosis, APC/C activity and the frequency of chromosome congression and segregation errors. In vitro manipulation and maturation of germ cells is widely used in both human and animal artificial reproduction techniques. Mammalian oocytes are naturally prone to chromosomal segregation errors, which are responsible for severe mental and developmental disorders. The data presented herein demonstrate that exposure of mouse oocytes to suboptimal temperature during manipulation and maturation could further increase the frequency of chromosome segregation defects in these cells.
ER  -

DANADOVÁ, Jitka, Natalie MATIJESCUKOVA, Anna Mac Gillavry DANYLEVSKA and Martin ANGER. Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature. \textit{Reproduction, Fertility and Development}. Clayton: CSIRO Publishing, 2017, vol.~29, No~5, p.~968-974. ISSN~1031-3613. Available from: https://dx.doi.org/10.1071/RD15306.

Detailed Information on Publication Record