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@article{1355521,
author = {Danadová, Jitka and Matijescukova, Natalie and Danylevska, Anna Mac Gillavry and Anger, Martin},
article_location = {Clayton},
article_number = {5},
doi = {http://dx.doi.org/10.1071/RD15306},
keywords = {anaphase-promoting complex/cyclosome; chromosome misalignment; culture temperature; IVM},
language = {eng},
issn = {1031-3613},
journal = {Reproduction, Fertility and Development},
title = {Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature},
url = {http://dx.doi.org/10.1071/RD15306},
volume = {29},
year = {2017}
}
TY - JOUR
ID - 1355521
AU - Danadová, Jitka - Matijescukova, Natalie - Danylevska, Anna Mac Gillavry - Anger, Martin
PY - 2017
TI - Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature
JF - Reproduction, Fertility and Development
VL - 29
IS - 5
SP - 968-974
EP - 968-974
PB - CSIRO Publishing
SN - 10313613
KW - anaphase-promoting complex/cyclosome
KW - chromosome misalignment
KW - culture temperature
KW - IVM
UR - http://dx.doi.org/10.1071/RD15306
L2 - http://dx.doi.org/10.1071/RD15306
N2 - Optimal culture conditions are essential for successful IVM of mammalian oocytes and for their further development into an embryo. In the present study we used live cell imaging microscopy to assess the effects of suboptimal culture temperature on various aspects of IVM, including duration of meiosis I, dynamics of polar body extrusion, chromosome congression, anaphase-promoting complex/cyclosome (APC/C) activation and aneuploidy. The data showed that even a small deviation from the optimal incubation temperature causes marked changes in the duration and synchronicity of meiosis, APC/C activity and the frequency of chromosome congression and segregation errors. In vitro manipulation and maturation of germ cells is widely used in both human and animal artificial reproduction techniques. Mammalian oocytes are naturally prone to chromosomal segregation errors, which are responsible for severe mental and developmental disorders. The data presented herein demonstrate that exposure of mouse oocytes to suboptimal temperature during manipulation and maturation could further increase the frequency of chromosome segregation defects in these cells.
ER -
DANADOVÁ, Jitka, Natalie MATIJESCUKOVA, Anna Mac Gillavry DANYLEVSKA and Martin ANGER. Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature. \textit{Reproduction, Fertility and Development}. Clayton: CSIRO Publishing, 2017, vol.~29, No~5, p.~968-974. ISSN~1031-3613. Available from: https://dx.doi.org/10.1071/RD15306.
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